Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
2330-AC-050

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Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125. When 0.25 µg/mL (100 µL/well) of rmOsteoactivin/Fc Chimera is immobilized on goat anti-mouse Fc Chimera antibody coated wells, it will support >30% adhesion of added SVEC4-10 cells (1 x 105 cells/well).
Source
Mouse myeloma cell line, NS0-derived mouse Osteoactivin/GPNMB protein
Mouse Osteoactivin
(Lys23 - Asn502)
Accession # Q8BVA0
IEGRMDP Mouse IgG2A
(Glu98 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Lys23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
80.3 kDa (monomer)
SDS-PAGE
130 kDa, reducing conditions

Product Datasheets

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2330-AC

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2330-AC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Osteoactivin/GPNMB

Osteoactivin (also named GPNMB and DC-HIL) is a 125 kDa, intracellular glycoprotein that is associated with cell endosomal/lysosomal compartments (1, 2). Mouse osteoactivin is synthesized as a type I, transmembrane, 574 amino acid (aa) precursor that contains a 22 aa signal sequence, a 478 aa luminal/extracellular domain, a 23 aa transmembrane segment and a 51 aa cytoplasmic tail. The luminal region contains an N-terminal heparin-binding motif, multiple glycosylation sites, an RGD motif and a 130 aa PKD domain. The intracellular tail also has an RGD motif, plus an ITAM (Y-x-x-I) and lysosomal targeting (L-L) motif. The extracellular/luminal region is 89% and 74% aa identical to the equivalent regions in rat and human, respectively. Cells known to express osteoactivin include osteoblasts, dendritic cells, and melanocytes, plus fetal chondrocytes and stratum basale keratinocytes (2, 3). Osteoactivin is reported to bind to heparan sulfate-proteoglycan, possibly on the surface of fibroblasts and endothelial cells (2). It may also interact with integrins.

References
  1. Bachner, D. et al. (2002) Gene Exp. Patterns 1:159.
  2. Shikano, S. et al. (2001) J. Biol. Chem. 276:8125.
  3. Owen, T.A. et al. (2003) Crit. Rev. Eukaryot. Gene Expr. 13:205.
Long Name
Glycoprotein Non-Metastatic Melanoma Protein B
Entrez Gene IDs
10457 (Human); 93695 (Mouse); 113955 (Rat); 102133517 (Cynomolgus Monkey)
Alternate Names
DC-HIL; glycoprotein (transmembrane) nmb; glycoprotein NMB; GPNMB; HGFIN; HGFINglycoprotein nmb-like protein; NMBosteoactivin; Osteoactivin; Transmembrane glycoprotein HGFIN; transmembrane glycoprotein NMB

Citations for Recombinant Mouse Osteoactivin/GPNMB Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Glycoprotein nonmetastatic melanoma protein B (GPNMB) promotes the progression of brain glioblastoma via Na(+)/K(+)-ATPase
    Authors: Hideaki Hara
    Biochem. Biophys. Res. Commun., 2016-11-09;481(2):7-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Allogeneic IgG combined with dendritic cell stimuli induce antitumour T-cell immunity.
    Authors: Carmi Y, Spitzer M, Linde I, Burt B, Prestwood T, Perlman N, Davidson M, Kenkel J, Segal E, Pusapati G, Bhattacharya N, Engleman E
    Nature, 2015-04-29;521(7550):99-104.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Bioassay

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