Recombinant Mouse PGLYRP1/PGRP-S His-tag Protein, CF Summary
Cys17-Glu182, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Peptidoglycan is immobilized at 5 μg/mL (100 µL/well), Recombinant Mouse PGLYRP1/PGRP-S His-tag Protein (Catalog # 2696-PGB) binds with an ED50 of 0.400‑4.80 ng/mL.
2 μg/lane of Recombinant Mouse PGLYRP1/PGRP-S His-tag Protein (Catalog # 2696-PGB) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-21 kDa.
The mouse PGRP family comprises four peptidoglycan recognition proteins that may function as innate immunity pattern recognition molecules (1, 2). Termed PGRP‑L, PGRP-I alpha, PGRP-I beta and PGRP-S, they are all products of separate genes, and all are named for the relative length of their translated product (3). PGRP-L (for long) is 530 amino acids (aa) in length, while PGRP-I alpha and I beta are intermediate (I) in length at 347 aa and 374 aa, respectively, and PGRP-S is shortest at 182 aa in length (3, 4). All mouse PGRPs bind peptidoglycan and Gram-positive bacteria, and all have at least three C-terminal PGRP domains at variable sites that are highly conserved from insects to mammals (3). Mouse PGRP-S, the first described member of the family, is an 18 kDa secreted protein associated with neutrophils (4, 5). The mature molecule is 166 aa in length and presumably contains three variably-sized peptide-carbohydrate recognition sequences. Mouse PGRP-S is 86%, 69% and 72% aa identical to rat, bovine and human mature PGRP‑S, respectively. Studies with PGRP-S deficient mice indicate that knock-out mice have increased susceptibility to infections with low (but not high) pathogenicity bacteria. Neutrophils from knock-out mice exhibit normal phagocytosis of bacteria but are defective in intracellular killing and digestion of nonpathogenic bacteria (5). The three longer PGRP members are all membrane-bound molecules that contain two membrane-spanning segments. Both the N- and C‑termini are depicted as being extracellular with a joining cytoplasmic domain. All three transmembrane forms show at least one PGRP domain on the C‑terminal extracellular region; other PGRP domains are variably distributed over their two extracellular and one cytoplasmic region (3).
- Girardin, S.E. and D.J. Philpott (2004) Eur. J. Immunol. 34:1777.
- Steiner, H. (2004) Immunol. Rev. 198:83.
- Kiselev, S.L. et al. (1998) J. Biol. Chem. 273:18633.
- Kang, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:10078.
- Dziarski, R. et al. (2003) Blood 102:689.
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