Recombinant Mouse pIgR Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Citations (3)
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Recombinant Mouse pIgR Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Mouse plgR is coated at 2 μg/mL (100 μL/well), the concentration of mouse IgM that produces 50% of the optimal binding response is 20-100 ng/mL.
Mouse myeloma cell line, NS0-derived mouse pIgR protein
Lys19-Lys645, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
70.1 kDa
93-97 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: pIgR

The mouse polymeric immunoglobulin receptor (plgR; also known as membrane secretory component) is a 115 kDa type I transmembrane glycoprotein that is synthesized as a 771 amino acid (aa) precursor. It includes an 18 aa signal sequence, a 627 aa extracellular domain (ECD) (aa 19‑645), a 23 aa transmembrane segment (aa 646‑668), and a 143 aa cytoplasmic region (aa 669‑771) (1‑3). The ECD consists of five V-type Ig-like domains and a sixth non-Ig domain that connects to the transmembrane region. The ECD of mouse plgR is 65%, 69%, 85%, 62% and 62% aa identical to the equivalent region in human, porcine, rat, bovine and canine, respectively. plgR is expressed on secretory epithelial cells and serves as a carrier that transports IgA and IgM across epithelium (1, 2, 4). On the basolateral surface of epithelial cells, the receptor initially binds non-covalently to IgA via domains #1 and #5 of the plgR. A rearrangement then occurs where a disulfide bond forms between domain #5 of the plgR and an IgA heavy chain (2). This complex is then internalized and transcytosed to the apical surface. A soluble covalent complex called secretory IgA (SIgA) is generated by proteolytic cleavage of the complex in the sixth extracellular domain of plgR and released into the lumen (5). This proteolytically generated plgR fragment is referred to as secretory component (SC). Notably, in human, plgR transcytoses constitutively, with or without ligand, creating both a bound and free, 78 kDa SC following cleavage (3). In mouse, this event would be expected to generate a 95 kDa fragment (1). The receptor component of the complex anchors the SIgA molecule to mucous (6), where it serves to protect mucous membranes that form a barrier between the interior of the body and the external environment (7).

  1. de Groot, N. et al. (1999) Transgenic Res. 8:125.
  2. Piskurich, J. et al. (1995) J. Immunol. 154:1735.
  3. Brandtzaeg, P. and F-E. Johansen (2001) Trends Immunol. 22:545.
  4. Ben-Hur, H. et al. (2004) Int. J. Mol. Med. 14:35.
  5. Asano, M. et al. (2004) Immunology 112:583.
  6. Phalipon, A. and B. Corthesy (2003) Trends Immunol. 24:55.
  7. Uren, T. et al. (2003) J. Immunol. 170:2531.
Long Name
Polymeric Immunoglobulin Receptor
Entrez Gene IDs
5284 (Human); 18703 (Mouse)
Alternate Names
FLJ22667; hepatocellular carcinoma associated protein TB6; Hepatocellular carcinoma-associated protein TB6; MGC125361; MGC125362; pIgR; Poly-Ig receptor; polymeric immunoglobulin receptor

Citations for Recombinant Mouse pIgR Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Enhancing IgG distribution to lung mucosal tissue improves protective effect of anti-Pseudomonas aeruginosa antibodies
    Authors: MJ Borrok, A DiGiandome, N Beyaz, GM Marchetti, AS Barnes, KJ Lekstrom, SS Phipps, MP McCarthy, H Wu, WF Dall'Acqua, P Tsui, R Gupta
    JCI Insight, 2018;3(12):.
    Applications: ELISA (Capture)
  2. Protection of Human Colon Cells from Shiga Toxin by Plant-based Recombinant Secretory IgA
    Authors: K Nakanishi, S Morikane, S Ichikawa, K Kurohane, Y Niwa, Y Akimoto, S Matsubara, H Kawakami, H Kobayashi, Y Imai
    Sci Rep, 2017;7(0):45843.
    Species: Plant
    Sample Types: Crude Leaf Extracts
    Applications: ELISA (Standard)
  3. pIgR and PECAM-1 bind to pneumococcal adhesins RrgA and PspC mediating bacterial brain invasion
    Authors: F Iovino, JY Engelen-Le, M Brouwer, D van de Bee, A van der En, M Valls Sero, P Mellroth, S Muschiol, J Bergstrand, J Widengren, B Henriques-
    J. Exp. Med., 2017;0(0):.
    Species: Mouse
    Sample Types: Complex Sample Type
    Applications: Bioassay


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