Recombinant Mouse PIR-B Protein, CF

R&D Systems | Catalog # 2754-PB

R&D Systems
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse PIR-B Protein (2754-PB)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

AAG01012

Applications

Bioactivity
View all PIR-B Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PIR-B protein
Gly24-Gly635, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly24

Predicted Molecular Mass

69 kDa

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When biotinylated recombinant rat MAG/Siglec-4a Fc Chimera is catured on Streptavidin coated plate (Catalog # CP003), Recombinant Mouse PIR-B binds with an apparent Kd < 100 nM.

Reviewed Applications

Read 1 review rated 3 using 2754-PB in the following applications:

Formulation, Preparation, and Storage

2754-PB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose
Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

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Background: PIR-B

Paired immunoglobulin‑like receptor B (PIR‑B) is a transmembrane protein that acts as a negative regulator of immune responses. It is the rodent homolog of human LILRB1‑5 family proteins. It is most closely related to LILRB3 which is also known as ILT5, LIR‑3, and CD85a. Like LILRB family members, PIR‑B contains cytoplasmic immunoreceptor tyrosine‑based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP‑1. PIR‑A and other LILRA family members instead contain cytoplasmic ITAMs which augment immune responses (1). Mature mouse PIR‑B is a 120 kDa glycoprotein that consists of a 618 amino acid (aa) extracellular domain (ECD) with six Ig‑like domains, a 21 aa transmembrane segment, and a 178 aa cytoplasmic domain with four ITIMs (2). The 85‑95 kDa human ILT5 contains four Ig‑like domains. Within common regions of their ECDs, mouse PIR‑B shares 73% and 55% aa sequence identity with rat PIR‑B and human ILT5, respectively. PIR‑B is expressed on dendritic cells, monocytes, macrophages, B cells, mast cells, neutrophils, eosinophils, basophils, osteoclasts, and cerebellar granule neurons (3‑10). Triggering of PIR‑B inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5‑7, 9). PIR‑B also inhibits the differentiation of osteoclasts and pro‑inflammatory M1 macrophages (4, 11). PIR‑B interacts in cis and in trans with MHC I molecules to limit autoreactive humoral and cellular immune responses (12‑14). It suppresses anti‑bacterial inflammatory responses by blocking TLR9‑dependent B cell activation (14). In the CNS, PIR‑B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (10). Upon binding to MAG, PIR‑B associates with TrkB and NGF R/p75 in cerebellar granule neurons (15).

References

  1. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
  2. Hayami, K. et al. (1997) J. Biol. Chem. 272:7320.
  3. Mitsuhashi, Y. et al. (2012) Blood 120:3256.
  4. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  5. Pereira, S. et al. (2004) J. Immunol. 173:5757.
  6. Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
  7. Ujike, A. et al. (2002) Nat. Immunol. 3:542.
  8. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA  100:1174.
  9. Sloane, D.E. et al. (2004) Blood 104:2832.
  10. Atwal, J.K. et al. (2008) Science 322:967.
  11. Ma, G. et al. (2011) Immunity 34:385.
  12. Nakamura, A. et al. (2004) Nat. Immunol. 5:623.
  13. Endo, S. et al. (2008) Proc. Natl. Acad. Sci. USA 38:14515.
  14. Kubo, T. et al. (2009) J. Exp. Med. 206:1971.
  15. Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.

Long Name

Paired-Ig-like Receptor B

Alternate Names

LILRB3, PIRB

Entrez Gene IDs

18733 (Mouse); 65146 (Rat)

Gene Symbol

PIRB

UniProt

AAG01012

Additional PIR-B Products

Product Documents for Recombinant Mouse PIR-B Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse PIR-B Protein, CF

For research use only

Related Research Areas

B Cell Activation

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