Recombinant Mouse PIR-B Protein, CF

Catalog # Availability Size / Price Qty
2754-PB-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse PIR-B Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When biotinylated recombinant rat MAG/Siglec-4a Fc Chimera is catured on Streptavidin coated plate (Catalog # CP003), Recombinant Mouse PIR-B binds with an apparent Kd < 100 nM.
Source
Mouse myeloma cell line, NS0-derived mouse PIR-B protein
Gly24-Gly635, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gly24
Predicted Molecular Mass
69 kDa
SDS-PAGE
85-95 kDa, reducing conditions

Product Datasheets

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2754-PB

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2754-PB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with trehalose
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: PIR-B

Paired immunoglobulin‑like receptor B (PIR‑B) is a transmembrane protein that acts as a negative regulator of immune responses. It is the rodent homolog of human LILRB1‑5 family proteins. It is most closely related to LILRB3 which is also known as ILT5, LIR‑3, and CD85a. Like LILRB family members, PIR‑B contains cytoplasmic immunoreceptor tyrosine‑based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP‑1. PIR‑A and other LILRA family members instead contain cytoplasmic ITAMs which augment immune responses (1). Mature mouse PIR‑B is a 120 kDa glycoprotein that consists of a 618 amino acid (aa) extracellular domain (ECD) with six Ig‑like domains, a 21 aa transmembrane segment, and a 178 aa cytoplasmic domain with four ITIMs (2). The 85‑95 kDa human ILT5 contains four Ig‑like domains. Within common regions of their ECDs, mouse PIR‑B shares 73% and 55% aa sequence identity with rat PIR‑B and human ILT5, respectively. PIR‑B is expressed on dendritic cells, monocytes, macrophages, B cells, mast cells, neutrophils, eosinophils, basophils, osteoclasts, and cerebellar granule neurons (3‑10). Triggering of PIR‑B inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5‑7, 9). PIR‑B also inhibits the differentiation of osteoclasts and pro‑inflammatory M1 macrophages (4, 11). PIR‑B interacts in cis and in trans with MHC I molecules to limit autoreactive humoral and cellular immune responses (12‑14). It suppresses anti‑bacterial inflammatory responses by blocking TLR9‑dependent B cell activation (14). In the CNS, PIR‑B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (10). Upon binding to MAG, PIR‑B associates with TrkB and NGF R/p75 in cerebellar granule neurons (15).

References
  1. Anderson, K.J. and R.L. Allen (2009) Immunology 127:8.
  2. Hayami, K. et al. (1997) J. Biol. Chem. 272:7320.
  3. Mitsuhashi, Y. et al. (2012) Blood 120:3256.
  4. Mori, Y. et al. (2008) J. Immunol. 181:4742.
  5. Pereira, S. et al. (2004) J. Immunol. 173:5757.
  6. Uehara, T. et al. (2001) J. Clin. Invest. 108:1041.
  7. Ujike, A. et al. (2002) Nat. Immunol. 3:542.
  8. Tedla, N. et al. (2003) Proc. Natl. Acad. Sci. USA  100:1174.
  9. Sloane, D.E. et al. (2004) Blood 104:2832.
  10. Atwal, J.K. et al. (2008) Science 322:967.
  11. Ma, G. et al. (2011) Immunity 34:385.
  12. Nakamura, A. et al. (2004) Nat. Immunol. 5:623.
  13. Endo, S. et al. (2008) Proc. Natl. Acad. Sci. USA 38:14515.
  14. Kubo, T. et al. (2009) J. Exp. Med. 206:1971.
  15. Fujita, Y. et al. (2011) Cell Death Dis. 2:e198.
Long Name
Paired-Ig-like Receptor B
Entrez Gene IDs
18733 (Mouse); 65146 (Rat)
Alternate Names
CD85a; HL9; ILT5; ILT-5; leukocyte immunoglobulin like receptor B3; LILRA6; LILRB3; LIR3; LIR-3; PIRB; PIR-B

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Recombinant Mouse PIR-B Protein, CF
By Anonymous on 09/01/2021
Application: Binding assay/Protein-protein interaction
Reason for Rating: Could not see cross reactivity of human HLA-G to murine PIR-B. Did see binding to human ILTs

Immobilize HLA-G and flow PIR-B