Recombinant Mouse PLTP Protein

Formulations:
Catalog # Availability Size / Price Qty
4918-PL-025
Product Details
Citations (1)
FAQs
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Reviews

Recombinant Mouse PLTP Protein Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit LPS-induced TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. 3.3 μg/mL of rmPLTP will inhibit 65-95% of the TNF-alpha secretion induced by 0.5 ng/mL of LPS.
Source
Mouse myeloma cell line, NS0-derived mouse PLTP protein
Glu18-Ala493, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Glu18
Predicted Molecular Mass
53.5 kDa
SDS-PAGE
70-85 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4918-PL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

4918-PL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLTP

Phospholipid transfer protein (PLTP; also known as lipid transfer protein II) is an 80 kDa, secreted glycoprotein, that belongs to the BPI/LBP/Plunc superfamily of molecules (1). It is synthesized as a precursor with a 17 amino acid (aa) signal sequence and a 476 aa mature chain. The mature chain contains two BPI/LBP/CETP domains (aa 21 - 239 and 263 - 463) and seven potential sites for N-linked glycosylation. Mature mouse PLTP shares 83% aa sequence identity with mature human PLTP (3). PLTP is expressed by a wide variety of tissues and is secreted by macrophages. Northern blot analysis shows the order of PLTP mRNA expression as follows: lung > adipose tissue, placenta, testis > brain > muscle, heart, liver (2). PLTP is a multifunctional, extracellular lipid transfer protein that plays a major role in lipoprotein metabolism and atherosclerosis (4 - 6). In addition to phospholipids, PLTP transfers several amphipatic compounds, including unesterified cholesterol, lipopolysaccharides, diacylglycerides, and alpha -tocopherol, the main isomer of vitamin E (4). According to several studies, PLTP not only affects plasma lipid and lipoprotein profiles, but also modulates cell physiology (4, 7 - 11). In mice, systemic PLTP deficiency proves to be atheroprotective, whereas PLTP overexpression is proatherogenic (4, 6). However, macrophage PLTP is shown to be atheroprotective (4, 6).

References
  1. Jauhiainen, M. and C. Ehnholm (2005) Methods 36:97.
  2. Jiang, X.-C. and C. Bruce (1995) J. Biol. Chem. 270:17133.
  3. Albers, J.J. et al. (1995) Biochim. Biophys. Acta. 1258:27.
  4. Ogier, N. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2407.
  5. Huuskonen, J. and C. Ehnholm (2000) Curr. Opin. Lipidol. 11:285.
  6. Valenta, D.T. et al. (2008) J. Lipid Res. 49:24.
  7. Jiang, X.-C. et al. (1998) J. Biol. Chem. 273:15714.
  8. Jiang, X.-C. et al. (2005) J. Biol. Chem. 280:18336.
  9. Desrumaux, C. et al. (2005) FASEB J. 19:296.
  10. Drouineaud, V. et al. (2006) FASEB J. 20:794.
  11. Klein, A. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26:2160.
Long Name
Phospholipid Transfer Protein
Entrez Gene IDs
5360 (Human); 18830 (Mouse); 296371 (Rat)
Alternate Names
HDLCQ9; Lipid transfer protein II; phospholipid transfer protein; PLTP

Citation for Recombinant Mouse PLTP Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Cathepsin G degradation of phospholipid transfer protein (PLTP) augments pulmonary inflammation.
    Authors: Brehm A, Geraghty P, Campos M, Garcia-Arcos I, Dabo A, Gaffney A, Eden E, Jiang X, D'Armiento J, Foronjy R
    FASEB J, 2014;28(5):2318-31.
    Species: Human
    Sample Types: BALF
    Applications: Bioassay

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