Measured by the ability of Recombinant Mouse Podocalyxin to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin.
Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature mouse Podocalyxin consists of a 383 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic domain (2). Within the ECD, mouse Podocalyxin shares 31% and 55% aa sequence identity with human and rat Podocalyxin, respectively. Alternative splicing of mouse Podocalyxin generates an additional isoform with a truncated intracellular region (2). Podocalyxin is expressed on glomerular and vascular endothelial cells (3, 4), neurons (5), hematopoietic, mesenchymal, and cardiac stem cells (6-9), and metastatic carcinoma and glioblastoma tumor cells (10-12). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (13). Podocalyxin promotes platelet activation (14), neurite outgrowth, branching, and synaptogenesis (5), homing of hematopoietic progenitor cells to the bone marrow and spleen (7), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (8, 9). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (4, 10, 11). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (3, 4, 11, 15). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (15).
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Entrez Gene IDs:
5420 (Human); 27205 (Mouse)
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL
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