Recombinant Mouse Podocalyxin Protein, CF

    
Datasheet / CoA / SDS
Product Details
FAQs
Supplemental Products
Reviews
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to inhibit the adhesion of CHO Chinese hamster ovary cells transfected with P-Selectin. The ED50 for this effect is 0.07-0.35 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse Podocalyxin protein
    His22-Arg402, with a C-terminal Asp and 10-His tag
  • Accession #
  • N-terminal Sequence
    Analysis
    His22
  • Predicted Molecular Mass
    41 kDa (monomer)
  • SDS-PAGE
    110-170 kDa, reducing conditions
Product Datasheets

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Certificate of Analysis Lookup

Certificate of Analysis Request Form

To download a Certificate of Analysis, please enter the catalog and lot numbers in the search box below.

Note: Certificate of Analysis not available for kit components.

1556-PD
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
Recombinant Mouse Podocalyxin (Catalog # 1556-PD) inhibits the adhesion of P-Selectin transfected CHO Chinese hamster ovary cells. The ED50 for this effect is
0.07-0.35 μg/mL.
Background: Podocalyxin
Podocalyxin (PODXL), also known as Podocalyxin-like protein 1 (PCLP-1), is a heavily glycosylated transmembrane sialoprotein in the CD34 and Endoglycan family (1). Mature mouse Podocalyxin consists of a 383 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic domain (2). Within the ECD, mouse Podocalyxin shares 31% and 55% aa sequence identity with human and rat Podocalyxin, respectively. Alternative splicing of mouse Podocalyxin generates an additional isoform with a truncated intracellular region (2). Podocalyxin is expressed on glomerular and vascular endothelial cells (3, 4), neurons (5), hematopoietic, mesenchymal, and cardiac stem cells (6-9), and metastatic carcinoma and glioblastoma tumor cells (10-12). A soluble form of Podocalyxin can be released into the urine of women with pre-eclampsia (13). Podocalyxin promotes platelet activation (14), neurite outgrowth, branching, and synaptogenesis (5), homing of hematopoietic progenitor cells to the bone marrow and spleen (7), and engraftment of mesenchymal and cardiac stem cells into ischemic heart and kidney (8, 9). Its interactions with L-Selectin and E-Selectin mediate the tethering of lymphocytes and metastatic tumor cells to the vascular endothelium (4, 10, 11). Podocalyxin can be expressed as a 160-165 kDa molecule or as even larger glycoforms (3, 4, 11, 15). In humans, an approximately 200 kDa glycoform contains the carbohydrate antigen recognized by TRA-1-60 and TRA-1-81 antibodies used for the identification of undifferentiated pluripotent embryonic stem cells (15).
  • References:
    1. Nielsen, J.S. and K.M. McNagny (2009) J. Am. Soc. Nephrol. 20:1669.
    2. Li, J. et al. (2001) DNA Seq. 12:407.
    3. Kershaw, D.B. et al. (1997) J. Biol. Chem. 272:15708.
    4. Sassetti, C. et al. (1998) J. Exp. Med. 187:1965.
    5. Vitureira, N. et al. (2010) PLoS ONE 8:e12003.
    6. Zhang, H. et al. (2014) Stem Cells 32:191.
    7. Doyonnas, R. et al. (2005) Blood 105:4170.
    8. Lee, R-H. et al. (2009) Blood 113:816.
    9. Moscoso, I. et al. (2013) J. Tissue Eng. Regen. Med. Epub. PMID 23897803.
    10. Dallas, M.R. et al. (2012) Am. J. Physiol. Cell Physiol. 303:C616.
    11. Thomas, S.N. et al. (2009) Am. J. Cell Physiol. 296:C505.
    12. Binder, Z.A. et al. (2013) PLoS ONE 8:e75945.
    13. Wang, Y. et al. (2012) Am. J. Physiol. Renal Physiol. 302:F1084.
    14. Alonso-Martin, S. et al. (2010) Thromb. Res. 125:e300.
    15. Schopperle, W.M. and W.C. DeWolf (2007) Stem Cells 25:723.
  • Entrez Gene IDs:
    5420 (Human); 27205 (Mouse)
  • Alternate Names:
    GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL
Related Research Areas

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs
Primary Antibodies
Description Application Cat# Citations Images  

His Tag Antibody

WB , Simple Western , Flow , AP , CyTOF-ready MAB050 28  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag HRP-conjugated Antibody

WB , Simple Western MAB050H 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag APC-conjugated Antibody

ICFlow IC050A  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PE-conjugated Antibody

ICFlow IC050P 3  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Biotinylated Antibody

WB BAM050 4  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Antibody

WB , Flow , AP MAB050R 2  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 488-conjugated Antibody

ICFlow IC050G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag PerCP-conjugated Antibody

ICFlow IC050C  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

His Tag Alexa Fluor® 700-conjugated Antibody

ICFlow IC050N  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Protein Purification Kits
Description Application Cat# Citations Images  

Histidine-tagged Protein Purification Resin

IP999 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Recombinant Mouse Podocalyxin Protein, CF?

Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Us to Order
reload

Feedback

Customer Information
Calculators

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷
R&D Systems Guarantee