Recombinant Mouse Prolactin R Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1309-PR-050
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Recombinant Mouse Prolactin R Fc Chimera Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.1 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Prolactin-induced proliferation of Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433. The ED50 for this effect is 1-5 ng/mL in the presence of 0.5 ng/mL of rhProlactin.
Source
Mouse myeloma cell line, NS0-derived mouse Prolactin R protein
Mouse Prolactin R
(Ser21 - Asp229)
Accession # Q08501
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
51 kDa (monomer)
SDS-PAGE
60-68 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1309-PR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Prolactin R

The neuroendocrine pituitary hormone Prolactin (PRL), also known as lactotrophin, mamotrophin, luteotropic hormone (LTH), or luteotropin, is a secreted hormone that affects reproduction and homeostasis in vertebrates. The functions of PRL can be placed in six broad categories: 1) reproduction and lactation; 2) growth and development; 3) endocrinology and metabolism; 4) brain and behavior; 5) immunomodulation; and 6) electrolyte balance (1, 2). PRL is secreted by the anterior pituitary gland, mammary gland, placenta, brain, uterus, decidua, dermal fibroblasts, B cells, T cells, NK cells, and some breast cancer cell lines. Although the major form of PRL is a 23 kDa monomeric protein, splice variants of 14, 16, and 22 kDa have been identified. PRL has also been found to be glycosylated, phosphorylated, dimerized, and polymerized. Glycosylation, phosphorylation, dimerization, or polymerization of PRL result in lower activity (2).

A single chain membrane-bound protein belonging to the class 1 cytokine superfamily mediates cell activation by PRL. The PRL receptor (PRL R) contains extracellular, transmembrane, and intracellular domains. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced. Although the cytoplasmic domains of the different isoforms vary in length and composition, their extracellular domains are identical. In mice, one long and three short forms of PRL receptor have been described (2). PRL receptors are found in mammary tissue, pituitary gland, brain, heart, lung thymus, spleen, liver, pancreas, kidney, adrenal gland, uterus, skeletal muscle and skin (3). A soluble form of PRL R containing the 206 NH2-terminal amino acids of the extracellular domain is secreted by mammary epithelial cells and is found in milk. Studies of PRL receptor deficient mice have demonstrated that PRL receptor signaling is required for female fertility and mammary development. Both male and female PRL receptor deficient mice have reduced bone formation and reduced abdominal fat deposits (1).

References
  1. Kelly, P.A. et al. (2001) Biochem. Society Transaction 29:48.
  2. Freeman, M.E. et al. (2000) Physiol. Rev. 80:1532.
  3. Nagano, M. and P.A. Kelly (1994) J. Biol. Chem. 269:1337.
Long Name
Prolactin Receptor
Entrez Gene IDs
5618 (Human); 19116 (Mouse); 24684 (Rat)
Alternate Names
delta 4-delta 7/11 truncated prolactin receptor; delta 4-SF1b truncated prolactin receptor; hPRL receptor; hPRLrI; PRLR; PRL-R; Prolactin R; prolactin receptor delta 7/11; prolactin receptor; ProlactinR; secreted prolactin binding protein

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