Recombinant Mouse Protein S/PROS1, CF

R&D Systems | Catalog # 9740-PS

R&D Systems
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Key Product Details

  • R&D Systems CHO-derived Recombinant Mouse Protein S/PROS1 (9740-PS)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q08761

Applications

Bioactivity
View all Protein S/PROS1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived mouse Protein S/PROS1 protein
Ala42-Arg669, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala42

Predicted Molecular Mass

71 kDa

SDS-PAGE

71-88 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Tyro3/Dtk Fc Chimera (Catalog # 759-DT) is immobilized at 1 μg/mL, 100 μL/well, Recombinant Mouse Protein S/PROS1 binds with an ED50 of 0.04-0.24 μg/mL.

Reviewed Applications

Read 1 review rated 4 using 9740-PS in the following applications:

Scientific Data Images for Recombinant Mouse Protein S/PROS1, CF

Recombinant Mouse Protein S/PROS1 Binding Activity

Recombinant Mouse Protein S/PROS1 Binding Activity

When Recombinant Mouse Dtk Fc Chimera (Catalog # 759-DT) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Mouse Protein S/PROS1 (Catalog # 9740-PS) binds with an ED50 of 0.04-0.24 µg/mL.

Formulation, Preparation, and Storage

9740-PS
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, and CaCl2.
Reconstitution

Reconstitute at 500 μg/mL in water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein S/PROS1

Anticoagulant Protein S (PROS1) is a 71 kDa plasma vitamin K-dependent glycoprotein characterized by the presence of the post-translational modification of specific glutamic acid residues to gamma-carboxyglutamic acid (Gla) in the N-terminal region. In addition to the N-terminal Gla domain, mature PROS1 contains a thrombin‑sensitive thumb loop, four tandem EGF-like domains and a C-terminal sex hormone-binding globulin (SHBG) domain composed of two Laminin G (LG) domains (1). Mouse PROS1 shares 80% aa sequence identity with human PROS1. Hundreds of mutations in humans have been reported throughout all the domains of the protein, typically resulting in PROS1 deficiency and an increase in the risk of thrombophilia (2, 3). PROS1 is expressed in many cell types supporting its reported involvement in multiple biological processes that include coagulation, apoptosis, cancer development and progression, and the innate immune response (4-9). PROS1 exists in plasma both in complex with C4b-binding protein (C4BP) (60%) and in a free form (40%). Both forms of PROS1 have anticoagulant activity either directly through inhibition of Factor X systems and prothrombinase while in complex with C4BP (5, 10) or in its well-established role as a cofactor of activated protein C (APC) inactivation of procoagulants FVa and FVIIIa (4). The free form of PROS-1 is also a ligand for a subfamily of receptor tyrosine kinases known as TAMs, which is composed of TYRO3, AXL, and MERTK (11). PROS-1 binds these tyrosine kinase receptors through its LG domains to activate downstream signaling pathways involved in tumor development and progression (7, 8, 11, 12).

References

  1. Dahlback, B. et al. (1986) Proc. Natl. Acad. Sci. 83:4199.
  2. Garcia de Frutos, P. et al. (2007) J. Thromb. Haemost. 98:543.
  3. Heeb, M. J. (2008) Expert Rev. Hematol. 1:9.
  4. Walker, F.J. (1984 Sem. Thromb. Hemosta. 10:131.
  5. Heeb, M. J. et al. (2004) J. Thromb. Haemost. 2:1766.
  6. Che Mat, M. F. (2016) Int. J. Oncol. 49:2359.
  7. Abboud-Jarrous, G. et al. (2017) Oncotarget. 8:13986.
  8. Qin, J. et al. (2016) Sci. Rep. 6:26662.
  9. Davra, V. et al. (2016) Cancers (Basel). 8:E107.
  10. Hackeng, T. M. et al. (1994) J. Biol. Chem. 269:21051.
  11. Tsou, W.I. et al. (2014) J. Biol. Chem. 289:25750.
  12. Linger, R.M.A. et al. (2008) Adv. Cancer Res. 100:35.

Alternate Names

PROS1

Entrez Gene IDs

5627 (Human); 19128 (Mouse); 81750 (Rat)

Gene Symbol

PROS1

UniProt

Q08761

Additional Protein S/PROS1 Products

Product Documents for Recombinant Mouse Protein S/PROS1, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse Protein S/PROS1, CF

For research use only

Citations for Recombinant Mouse Protein S/PROS1, CF

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