Mouse Prss28, also known as Implantation Serine Protease 1 (ISP1), belongs to the S1 serine proteinase family with a conserved Histidine-Aspartic Acid-Serine catalytic triad (1). It closely resembles the mouse mast cell tryptases, specifically MCP-6 and MCP-7 (1). It is co-expressed with ISP2/Prss29 in pre-implantation embryos and uterus (2). It is involved with embryo hatching and its activity is important for successful implantation (2).
Recombinant Mouse Prss28 Protein, CF
R&D Systems | Catalog # 4808-SE
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Key Product Details
- R&D Systems NS0-derived Recombinant Mouse Prss28 Protein (4808-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
NS0
Accession Number
Structure / Form
Pro form
Applications
Enzyme Activity
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Product Specifications
Source
Mouse myeloma cell line, NS0-derived mouse PRSS28 protein
Ala19-Gln274, with a C-terminal 10-His tag
Accession # Q924N9
Ala19-Gln274, with a C-terminal 10-His tag
Accession # Q924N9
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala19
Predicted Molecular Mass
30 kDa
SDS-PAGE
34 kDa, reducing conditions
Activity
Measured by its ability to cleave a peptide substrate, Suc-VPF-SBzl after activation by Thermolysin.
The specific activity is >15,000 pmol/min/µg, as measured under the described conditions.
The specific activity is >15,000 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
4808-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PRSS28
References
- Tang, L. and D. E. Rancourt (2005) Gene 364:30.
- Sharma, N. et al. (2006) BMC Developmental Biology 6:61.
Long Name
Protease, Serine, 28
Alternate Names
ISP1
Entrez Gene IDs
114661 (Mouse)
Gene Symbol
PRSS28
UniProt
Additional PRSS28 Products
Product Documents for Recombinant Mouse Prss28 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Prss28 Protein, CF
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse Prss28 Protein, CF (4808-SE):
Materials
- Activation Buffer: 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% Brij-35, pH 7.5 (TCNB)
- Assay Buffer: 50 mM Tris, 150 mM NaCl, pH 8.0
- Recombinant Mouse PRSS28 (rmPRSS28) (Catalog # 4808-SE)
- Bacterial Thermolysin (Catalog # 3097-ZN)
- 1,10-Phenanthroline (Sigma, Catalog # 320056), 600 mM stock in DMSO
- Substrate: SUC-Val-Pro-Phe-SBZL (Bachem, Catalog # M-1885), 10 mM stock in DMSO
- 5,5’-dithio-bis(2-nitrobenzoic acid) (DTNB) (Sigma, Catalog # D-8130), 10 mM stock in DMSO
- 96 well clear plate (Costar, Catalog # 92592)
- Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
- Dilute rmPRSS28 to 100 µg/mL with 0.1 µg/mL Thermolysin in Activation Buffer.
- Incubate at 37 °C for 60 minutes.
- Stop Thermolysin activity by adding 1,10-Phenanthroline to 10 mM.
- Incubate at room temperature for 15 minutes.
- Dilute active rmPRSS8 to 0.05 µg/mL in Assay Buffer.
- Dilute Substrate to 200 µM with 200 µM DTNB in Assay Buffer.
- Load 50 µL of the 0.05 µg/mL rmPRSS28 into a clear microplate. Include a Substrate Blank containing 50 µL of Assay Buffer.
- Start the reaction by adding 50 µL of the Substrate Mixture into wells.
- Read in kinetic mode for 5 minutes at an absorbance of 405 nm.
- Calculate specific activity using the following formula:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (OD/min) x well volume (L) x 1012 pmol/mol |
| ext. coeff** (M-1cm-1) x path corr.*** (cm) x amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Using the extinction coefficient 13260 M-1cm-1
***Using the path correction 0.320 cm
Note: the output of many spectrophotometers is in mOD. Per Well:
- rmPRSS8: 2.5 ng
- Substrate: 100 µM
- DTNB: 100 µM