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Bacterial Thermolysin Protein, CF

R&D Systems | Catalog # 3097-ZN

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Key Product Details

  • R&D Systems G. stearothermophilus-derived Bacterial Thermolysin Protein (3097-ZN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

G. stearothermophilus

Applications

Enzyme Activity
View all Thermolysin Proteins and Enzymes »

Product Specifications

Source

Geobacillus stearothermophilus-derived Thermolysin protein

Purity

>70%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

SDS-PAGE

36 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK(Dnp)-OH (Catalog # ES005).
The specific activity is >12,000 pmol/min/µg, as measured with 10 µM ES005 and 2 ng enzyme in 100 µL of 50 mM Tris, 10 mM CaCl2, 0.15 M NaCl, 0.05% Brij 35, pH 7.5.

Reviewed Applications

Read 4 reviews rated 4.8 using 3097-ZN in the following applications:

Formulation, Preparation, and Storage

3097-ZN
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, CaCl2 and Glycerol.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Thermolysin

Thermolysin is a zinc protease that can be used to activate a number of proteases produced at R&D Systems (see product inserts for details). Its activity can be inhibited by phosphoramidon, 1,10-phenanthroline or EDTA.

Protease Catalog # [Thermolysin] Inhibitor
rhADAM8 1031-AD 0.75 μg/mL Phosphoramidon
rhEnterokinase 1585-SE 1.58 μg/mL 1, 10-Phenanthroline
rhFactor VII 2338-SE 6.25 μg/mL 1, 10-Phenanthroline
rhFactor XI 2460-SE 10 μg/mL EDTA
rhHGF Activator 1514-SE 4.3 μg/mL 1, 10-Phenanthroline
rhKallikrein 1 2337-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 3/PSA 1344-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 4 1719-SE 1.0 μg/mL 1, 10-Phenanthroline
rhKallikrein 7 2624-SE 10 μg/mL EDTA
rhKallikrein 11 1595-SE 10 μg/mL EDTA
rhKallikrein 14 2626-SE 10 μg/mL EDTA
rmMast Cell Protease-11 2857-SE 0.2 μg/mL 1, 10-Phenanthroline
rhPlasma Kallikrein 2497-SE 10 μg/mL EDTA
rmPlasma Kallikrein 2498-SE 10 μg/mL EDTA
hThrombin 2196-SE 42 μg/mL 1, 10-Phenanthroline
rmTryptase-5 2634-SE 0.2 μg/mL 1, 10-Phenanthroline
rmTryptase epsilon 2059-SE 0.3 μg/mL 1, 10-Phenanthroline
rhTryptase beta 2 3796-SE 0.1 μg/mL 1, 10-Phenanthroline
rmFactor VII 3178-PA 1.0 μg/mL 1, 10-Phenanthroline
rhProprotein Convertase 7 2984-SE 2.0 μg/mL 1, 10-Phenanthroline
rm Prss28 4808-SE 0.1 μg/mL 1, 10-Phenanthroline
rhFactor XIV/Protein C 3349-SE 2.3 μg/mL 1, 10-Phenanthroline
rmFactor XIV/Protein C 4885-SE 5.0 μg/mL 1, 10-Phenanthroline

Additional Thermolysin Products

Product Documents for Bacterial Thermolysin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Bacterial Thermolysin Protein, CF

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Bacterial Thermolysin Protein, CF

Customer Reviews for Bacterial Thermolysin Protein, CF (4)

4.8 out of 5
4 Customer Ratings
5 Stars
75%
4 Stars
25%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Bacterial Thermolysin Protein, CF
    Name: Amar Singh
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 01/20/2023
  • Bacterial Thermolysin Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/13/2020
    used to activate pro-KLK7 in an in vitro assay
    Bacterial Thermolysin Protein, CF 3097-ZN
  • Bacterial Thermolysin Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 03/22/2018
  • Bacterial Thermolysin Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 08/10/2017

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