Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10333-TK-050
Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF Binding Activity
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Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324). The ED50 for this effect is 8-48 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse PTK7/CCK4 protein
Mouse PTK7/CCK4
(Ala23-Thr696)
Accession # Q8BKG3
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ala23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
102 kDa
SDS-PAGE
110-130 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10333-TK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324). The ED50 for this effect is 8-48 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-160 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a member of the receptor tyrosine kinase superfamily (1, 2). PTK7 is a type I transmembrane receptor with a large extracellular domain (ECD) containing 7 Ig-like C2 type loops, a transmembrane domain, and a cytoplasmic tyrosine kinase homology domain. However, due to the lack of the DFG triplet motif required for catalytic activity in the kinase domain, PTK7 is considered a pseudokinase (1, 3). The mature ECD of mouse PTK7 shares 92% amino acid sequence identity with human PTK7. PTK7 is expressed in a wide array of tissue types ranging from lung and liver to kidney and placenta and has been linked to a broad range of functions (5). While originally identified as being over-expressed in colon carcinomas, PTK7 has been shown to play a role in embryogenesis, epithelial tissue organization, angiogenesis, cell motility, and survival (1, 6, 7). PTK7 has been shown to be an important regulator of the Wnt signaling pathways, both canonical and non-canonical, and is linked to the regulation of the planar cell polarity pathway (3, 6). Proteolysis of full-length PTK7 by MMPs (matrix metalloproteinases), ADAMs (a disintegrin domain and metalloproteinases), and gamma-secretases results in a soluble N-terminal fragment and several C-terminal, membrane-associated or intracellular proteolytic fragments (3, 6). The soluble form is a co-receptor for the Semaphorin/Plexin and VEGF signaling pathways (8). Deregulation of PTK7 signaling has now been observed in numerous cancers including colon, gastric, lung, and acute myeloid leukemia (1, 3). Recent studies have shown that PTK7 expression promoted increased migration and resistance to apoptosis in leukemic cells and acute myeloid leukemia (AML) blasts, while knock-down of PTK7 induced apoptosis in colorectal carcinoma cells (2, 7). Further, other studies have suggested that the ratio of full-length vs. cleaved protein, not just expression, contributes to PTK7's metastatic effects in cancer (6).

References
  1. Shin, W. et al. (2008) Biochem. Bioph. Res. Co. 371:793.
  2. Meng, L. et al. (2010) PLoS ONE 5:e14018.
  3. Golubkov, V. et al. (2010) J. Biol. Chem. 285:35740.
  4. Berger, H. et al. (2017) Front. Cell Dev. Biol. 5:31.
  5. Park, S. et al. (1996) J. Biochem. 119:235.
  6. Golubkov, V. et al. (2014) J. Biol. Chem. 289:24238.
  7. Prebet, T. et al. (2010) Blood. 116:2315.
  8. Peradziryi, H. et al. (2012) Arch. Biochem. Biophys. 524:71.
Long Name
Protein Tyrosine Kinase 7
Entrez Gene IDs
5754 (Human); 71461 (Mouse); 301242 (Rat)
Alternate Names
CCK4; CCK-4; CCK4Colon carcinoma kinase 4; colon carcinoma kinase-4; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; PTK7; Tyrosine-protein kinase-like 7

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