Recombinant Mouse Ryk Fc Chimera Protein, CF

  
  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to bind biotinylated recombinant mouse Wnt-3a in a functional ELISA with an estimated KD <50 nM.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse Ryk
    (Arg35-Arg211)
    Accession # Q01887
    IEGRMDP Mouse IgG2A
    (Glu98-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Arg35
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    47 kDa (monomer)
  • SDS-PAGE
    60-66 kDa, reducing conditions
4649-RK
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 300 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ryk

Ryk (Related to tyrosine kinase), also called VIK in mouse, is an 70‑90 kDa type I transmembrane glycoprotein that is an atypical member of the receptor tyrosine kinase superfamily (1, 2). Mouse Ryk cDNA encodes 594 amino acids (aa) that include a 34 aa signal sequence, a 177 aa extracellular domain (ECD) that contains a WIF (Wnt Inhibitory Factor) domain (aa 50‑178), a transmembrane sequence, and a cytoplasmic region with a non‑functional Ser/Thr protein kinase domain (aa 317‑590) (1‑3). The cytoplasmic region participates in signaling by undergoing gamma ‑secretase cleavage and translocating to the nucleus in response to Wnts (4). Alternate start sites at aa 28 or aa 117 have been reported. Within the ECD, mouse Ryk shares 94% and 100% aa sequence identity with mouse and rat Ryk, respectively. Ryk protein expression is reported in developing neurons of the corticospinal tract, ventral zone, and retina, as well as adult tissue epithelia, stroma and blood vessels (4‑9). Ryk binds Wnts and EphB2/B3 receptors via its WIF domain (8‑10). It may form a Wnt receptor complex with Frizzled receptors, serving as a link between Wnt and Dishevelled and converting Wnt/Frizzled attraction signals to repulsion signals (6‑8, 10). In particular, Ryk is required for Wnt5a‑induced axon guidance after cortical axons cross the corpus callosum, and for Wnt3‑mediated guidance of developing retinal ganglion cells (7, 8). Mice deleted for the Ryk gene show defects in axon guidance that produce craniofacial defects and shortened limbs (10, 11). Ryk-/- mice share a cleft palate phenotype with EphB2/B3‑deleted mice (10). Ryk is often over‑expressed in human ovarian cancer (5).

  • References:
    1. Hovens, C.M. et al. (1992) Proc. Natl. Acad. Sci. USA 89:11818.
    2. Clark, C.E.J. et al. (2012) Neurosignals 20:202.
    3. Katso, R.M. et al. (1999) Mol. Cell. Biol. 19:6427.
    4. Lyu, J. et al. (2008) Dev. Cell 15:773.
    5. Katso, R.M. et al. (1999) Cancer Res. 59:2265.
    6. Liu, Y. et al. (2005) Nat. Neurosci. 8:1151.
    7. Schmitt, A.M. et al. (2006) Nature 439:31.
    8. Keeble, T.R. et al. (2006) J. Neurosci. 26:5840.
    9. Kamitori, K. et al. (2005) Biochem. Biophys. Res. Commun. 330:446.
    10. Lu, W. et al. (2004) Cell 119:97.
    11. Halford, M.M. et al. (2000) Nat. Genet. 25:414.
  • Long Name:
    Receptor Type Tyrosine Protein Kinase RYK
  • Entrez Gene IDs:
    6259 (Human); 20187 (Mouse); 140585 (Rat)
  • Alternate Names:
    D3S3195; EC 2.7.10; EC 2.7.10.1; hydroxyaryl-protein kinase; JTK5; JTK5A protein tyrosine kinase; JTK5A; RYK receptor-like tyrosine kinase; Ryk; RYK1; tyrosine-protein kinase RYK
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