Recombinant Mouse S100A8 Protein, CF

Catalog # Availability Size / Price Qty
9877-S8-050
Recombinant Mouse S100A8 Protein, CF Data
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Recombinant Mouse S100A8 Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce CXCL1/KC secretion by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-9 μg/mL.
Source
E. coli-derived mouse S100A8 protein
Met1-Glu89
Accession #
N-terminal Sequence
Analysis
Met1 & Pro2
Predicted Molecular Mass
10 kDa
SDS-PAGE
7 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9877-S8

Formulation Lyophilized from a 0.2 μm filtered solution in Tris and TCEP with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Mouse S100A8 (Catalog # 9877-S8) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50for this effect is 1.5-9 μg/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Mouse S100A8 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 7 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8

Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins (1, 2). Mouse S100A8 is an 89 amino acid protein that contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 forms homodimer, and also heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers (3, 4). The dimeric S100 A8/A9 complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. Like S100A9, S100A8 is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (5-7). It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. In addition, S100A8 was shown to activate lung alveolar epithelial cells, osteoclasts, and chondrocytes in TLR4-dependent manner, inducing proinflammatory cytokines and chemokines including, IL-6, IL-8, KC, and MCP-1 (8-10). In osteoarthritic chondrocytes, S100A8 and A9 also promoted expression of matrix metalloproteinases (MMP-1, -3, -9, and -13) potentially affecting to cartilage breakdown (10). The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively (11, 12).

References
  1. Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
  2. Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822.
  3. Ryckman, C. et al. (2003) J. Immunol. 170:3233.
  4. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298.
  5. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
  6. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
  7. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
  8. Chakraborty D. et al. (2017) Front Immunol. 8:1493
  9. Grevers L.C. Arthritis Rheum. 63:1365.
  10. Schelbergen R.F. Arthritis Rheum. 64:1477.
  11. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
  12. Lackmann, M. et al. (1993) J. Immunol. 150:2981.
Long Name
S100 Calcium Binding Protein A8
Entrez Gene IDs
6279 (Human); 20201 (Mouse)
Alternate Names
60B8AG; CAGACP-10; Calgranulin A; calgranulin-A; Calprotectin L1L subunit; CFAG; CFAGL1Ag; CGLA; Cystic fibrosis antigen; Leukocyte L1 complex light chain; MA387; MIF; Migration inhibitory factor-related protein 8; MRP8; MRP-8; MRP8S100 calcium binding protein A8 (calgranulin A); NIF; P8; protein S100-A8; S100 calcium binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A8; S100A8; Urinary stone protein band A

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