Recombinant Mouse S100A8 Protein, CF Summary
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris and TCEP with Trehalose.|
|Reconstitution||Reconstitute at 250 μg/mL in water.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse S100A8 (Catalog # 9877-S8) induces CXCL1/KC secretion in C3H10T1/2 mouse embryonic fibroblast cells. The ED50for this effect is 1.5-9 μg/mL.
1 μg/lane of Recombinant Mouse S100A8 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 7 kDa.
Mouse S100A8, also known as CP-10, Calgranulin A and MRP8, is a 10 kDa member of the S100 family of calcium-binding proteins (1, 2). Mouse S100A8 is an 89 amino acid protein that contains short sequential modules, including an N-terminal alpha -helix, a Ca++-binding EF-hand segment, a short central linker region, a second EF-hand segment, and a C-terminal alpha -helix. S100A8 forms homodimer, and also heterodimerizes with S100A9. In the presence of Ca++, the heterodimers form heterotetramers (3, 4). The dimeric S100 A8/A9 complex is found both intracellularly and extracellularly. It binds to heparan sulfate and is chemotactic for PMNs and macrophages. Like S100A9, S100A8 is up-regulated in neutrophils and monocytes at sites of inflammation (e.g. psoriasis, rheumatoid arthritis, cardiac ischemia) and are present at elevated concentrations in rheumatoid arthritis synovial fluid (5-7). It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. In addition, S100A8 was shown to activate lung alveolar epithelial cells, osteoclasts, and chondrocytes in TLR4-dependent manner, inducing proinflammatory cytokines and chemokines including, IL-6, IL-8, KC, and MCP-1 (8-10). In osteoarthritic chondrocytes, S100A8 and A9 also promoted expression of matrix metalloproteinases (MMP-1, -3, -9, and -13) potentially affecting to cartilage breakdown (10). The amino acid sequence of mouse S100A8 is 80% and 57% identical to that of rat and human S100A8, respectively (11, 12).
- Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223.
- Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822.
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- Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371.
- Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69.
- Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250.
- Chakraborty D. et al. (2017) Front Immunol. 8:1493
- Grevers L.C. Arthritis Rheum. 63:1365.
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- Lagasse, E. and I.L. Weissman (1992) Blood 79:1907.
- Lackmann, M. et al. (1993) J. Immunol. 150:2981.
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