>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When
Mouse CD7 (Catalog # 8145-CD)
is immobilized at 1 μg/mL, 100 μL/well, the concentration of
Recombinant Mouse SECTM1A Fc Chimera that produces 50% of
is 30-150 ng/mL.
Mouse myeloma cell line, NS0-derived mouse SECTM1A protein
When Recombinant Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 µg/mL, Recombinant Mouse SECTM1A Fc Chimera (Catalog # 7837-ST) binds with an ED50 of 30‑150 ng/mL.
SECTM1A (secreted and transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).
Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
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