Recombinant Mouse SECTM1A Fc Chimera Protein, CF

    
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  • Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Mouse SECTM1A Fc Chimera that produces 50% of the optimal binding response is 30-150 ng/mL.
  • Source
    Mouse myeloma cell line, NS0-derived mouse SECTM1A protein
    Mouse SECTM1A
    (Gln28-Thr165)
    Accession # NP_663348
    IEGRMDP Mouse IgG2A
    (Glu98-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Gln28 predicted: No results obtained, sequencing might be blocked
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    42.6 kDa (monomer)
  • SDS-PAGE
    55-66 kDa, reducing conditions
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7837-ST
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 100 μg/mL in PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Image
When Recombinant Recombinant Mouse CD7 (Catalog # 8145-CD) is immobilized at 1 µg/mL, Recombinant Mouse SECTM1A Fc Chimera (Catalog # 7837-ST) binds with an ED50 of 30‑150 ng/mL.
Background: SECTM1A
SECTM1A (secreted and transmembrane 1A), is 192 amino acid (aa) protein that shares approximately 45‑51%, 81%, and 43% aa sequence identity with human SECTM1 (also called K12), rat SECTM1, and mouse SECTM1B/K12, respectively, and appears to share structural and functional characteristics with other SECTM1 proteins. Human SECTM1 can be found either found as an approximately 27 kDa intracellular type I transmembrane protein that shows a perinuclear, Golgi‑like staining pattern, or as a 20 kDa soluble, secreted form, and is produced by some myeloid cells and by thymic epithelia and fibroblasts (1‑3). Stimulation with IFN‑ gamma is often necessary to detect human SECTM1 expression, and it is thought to be an interferon early‑response gene (1‑5). Mouse SECTM1A cDNA encodes a signal sequence, an extracellular domain with four potential N‑linked glycosylation sites, a transmembrane sequence, and a very short (approximately 6 aa) cytoplasmic sequence (6). SECTM1 proteins from human and mouse show species‑specific binding of CD7 and co‑stimulation of T cells, including enhancement of CD3‑induced proliferation (2‑4).
  • References:
    1. Slentz-Kesler, K.A. et al. (1998) Genomics 47:327.
    2. Lam, G.K. et al. (2005) J. Clin. Immunol. 25:41.
    3. Wang, T. et al. (2012) J. Leukoc. Biol. 91:449.
    4. Lyman, S.D. et al. (2000) J. Biol. Chem. 275:3431.
    5. Huyton, T. et al. (2011) Biochim. Biophys. Acta 1810:1294.
    6. Swiss-Prot Accession # Q921W8.
  • Long Name:
    Secreted and Transmembrane 1A
  • Entrez Gene IDs:
    209588 (Mouse); 287885 (Rat)
  • Alternate Names:
    SECTM1A
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Reconstitution Buffers
Description Application Cat# Citations Images  

Reconstitution Buffer 1 (PBS)

RB01
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