Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF Summary
|MHHHHHH||Mouse Semaphorin 3C
(Gln24-Ser741, Arg548Ala, Arg552Ala)
Accession # Q62181
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid, NaCl and Tween® 20.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated) (Catalog # 1728‑S3) supports the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 0.12-1.2 μg/mL.
Background: Semaphorin 3C
Semaphorin 3C (Sema3C; previously semaE) is one of six Class 3 secreted semaphorins which share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon and/or vascular guidance during development, and may be upregulated in tumor progression (1, 2). The 751 amino acid (aa) mouse Sema3C is highly modular. It contains a 20 aa signal sequence, an ~500 aa N-terminal Sema domain that forms a beta -propeller structure similar to that found in integrin molecules, a cysteine knot, a furin-type cleavage site, an Ig-like domain, and a C-terminal basic domain (1-3). Covalent dimerization plus cleavage at the C-terminus are required for activity of class 3 semaphorins (4). Mouse Sema3C shares at least 95% aa identity with human, rat, cow and dog Sema3C, and 89% and 75% aa identity with chick and zebrafish Sema3C, respectively. Type 3 semaphorins transduce signals through transmembrane plexins, either directly or by binding associated neuropilin receptors (1, 2). Sema3C signaling is transduced by Plexin-D1 indirectly via neuropilin-1 or neuropilin-2 receptors (5). Sema3C is expressed in all somitic motor neurons, in lung buds and in cardiac neural crest cells during development (1, 5-8). Sema3C activates integrins in certain cells so, in addition to its repulsive activities, it sometimes acts as a chemoattractant (6, 9). In the developing nervous system, this chemoattraction appears to complement Sema3A repulsion in adjacent cell layers (1, 6, 7). Sema3C also provides an attractive force opposing Sema6A and Sema6B to guide migration of neural crest endothelial cells to the cardiac outflow tract (10). Consequently, defects in aortic arch formation occur when Sema3C or Plexin-D1 genes or Sema3C-neuropilin interactions are disrupted (5, 11, 12).
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- Herman, J. G. and G. G. Meadows (2007) Int. J. Oncol. 30:1231.
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- Feiner, L. et al. (2001) Development 128:3061.
- Gu, C. et al. (2003) Dev. Cell 5:45.
Citations for Recombinant Mouse Semaphorin 3C Fc Chimera (Truncated), CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits
Authors: K Burk, E Mire, A Bellon, M Hocine, J Guillot, F Moraes, Y Yoshida, M Simons, S Chauvet, F Mann
Nat Commun, 2017;8(0):14508.
Sample Types: Whole Cells
Neural crest-derived SEMA3C activates endothelial NRP1 for cardiac outflow tract septation.
Authors: Plein A, Calmont A, Fantin A, Denti L, Anderson N, Scambler P, Ruhrberg C
J Clin Invest, 2015;125(7):2661-76.
Sample Types: Whole Tissue
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