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Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

Catalog #: 3238-S3
5 Citations Datasheet / COA / SDS

Discontinued Product

3238-S3 has been discontinued.
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Citations (5)
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Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 2.5‑5.0 µg/mL of Recombinant Mouse Semaphorin 3E Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta ‑NGF (Catalog # 256-GF).
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Semaphorin 3E protein
Mouse Semaphorin 3E
Asn27-Phe766 (Arg557Ala and Arg560Ala)
Accession # P70275		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
P70275
N-terminal Sequence
Analysis
Asn27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
112 kDa (monomer)
SDS-PAGE
110-120 kDa, reducing conditions

Product Datasheets

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3238-S3

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3238-S3

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Semaphorin 3E

Semaphorin 3E (Sema3E), previously known as SemaH, is one of six Class 3 (secreted) semaphorins which function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E contains a seven-blade beta -propeller sema domain, a cysteine-knot PSI domain, an Ig-like domain, and a basic region. Dimerization and cleavage within the basic region are required for the repulsing activity of class 3 semaphorins (2). Sema3E can also be cleaved at a furin consensus sequence C-terminal to the sema domain, resulting in a 61 kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (3, 4). Mature mouse Sema3E shares 90% and 98% aa sequence identity with human and rat Sema3E, respectively. Sema3E signaling is transduced by Plexin D1 which may also be associated with Neuropilin 1 and/or VEGF R2 (2, 5, 6). Its interaction with Plexin D1 inhibits axon migration in the neocortex and forebrain (6, 7), although it can attract axons that express both Plexin D1 and Neuropilin 1 (6). Sema3E promotes axonal growth (5), the development of glutamatergic synaptic specificity (8, 9), and the development of GnRH producing neurons (10). Genetic disruption of either Sema3E or Plexin D1 in mouse causes excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (11, 12). In addition, Sema3E is up-regulated by inflammatory macrophages and damaged hepatocytes (13-15). It inhibits smooth muscle cell proliferation and migration in the asthmatic airway (16), promotes hepatic stellate cell activation and wound healing (15), and regulates the migration of developing thymocytes (17).

References
  1. Oh, W.J. and C. Gu (2013) Semin. Cell Dev. Biol. 24:156.
  2. Adams, R. H. et al. (1997) EMBO J. 16:6077.
  3. Christensen, C. et al. (2005) Cancer Res. 65:6167.
  4. Casazza, A. et al. (2010) J. Clin. Invest. 120:2684.
  5. Bellon, A. et al. (2010) Neuron 66:205.
  6. Chauvet, S. et al. (2007) Neuron 56:807.
  7. Bribian, A. et al. (2014) Nat. Commun. 5:4265.
  8. Ding, J.B. et al. (2011) Nat. Neurosci. 15:215.
  9. Pecho-Vrieseling, E. et al. (2009) Nature 459:842.
  10. Cariboni, A. et al. (2015) J. Clin. Invest. 125:2413.
  11. Gu, C. et al. (2005) Science 307:265.
  12. Gitler, A. D. et al. (2004) Developmental Cell 7:107.
  13. Wanschel, A. et al. (2013) Arterioscler. Thromb. Vasc. Biol. 33:886.
  14. Shimizu, I. et al. (2013) Cell Metab. 18:491.
  15. Yagai, T. et al. (2014) Am. J. Pathol. 184:2250.
  16. Movassagh, H. et al. (2014) J. Allergy Clin. Immunol. 133:560.
  17. Choi, Y.I. et al. (2014) Proc. Natl. Acad. Sci. USA 111:379.
Entrez Gene IDs
9723 (Human); 20349 (Mouse)
Alternate Names
(semaphorin) 3E; coll-5; KIAA0331M-SEMAH; sema domain, immunoglobulin domain (Ig), short basic domain, secreted; Sema3E; SEMAH; SEMAHM-SemaK; Semaphorin 3E; semaphorin-3E

Citations for Recombinant Mouse Semaphorin 3E Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. The guidance receptor plexin D1 is a mechanosensor in endothelial cells
    Authors: V Mehta, KL Pang, D Rozbesky, K Nather, A Keen, D Lachowski, Y Kong, D Karia, M Ameismeier, J Huang, Y Fang, A Del Rio He, JS Reader, EY Jones, E Tzima
    Nature, 2020-02-05;578(7794):290-295.
    Species: Bovine
    Sample Types: Cells
    Applications: Cell Culture
  2. Post-endocytic sorting of Plexin-D1 controls signal transduction and development of axonal and vascular circuits
    Authors: K Burk, E Mire, A Bellon, M Hocine, J Guillot, F Moraes, Y Yoshida, M Simons, S Chauvet, F Mann
    Nat Commun, 2017-02-22;8(0):14508.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Semaphorin 3E secreted by damaged hepatocytes regulates the sinusoidal regeneration and liver fibrosis during liver regeneration.
    Authors: Yagai T, Miyajima A, Tanaka M
    Am J Pathol, 2014-06-12;184(8):2250-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Neuroimmune guidance cue Semaphorin 3E is expressed in atherosclerotic plaques and regulates macrophage retention.
    Authors: Wanschel, Amarylis, Seibert, Tara, Hewing, Bernd, Ramkhelawon, Bhama, Ray, Tathagat, van Gils, Janine M, Rayner, Katey J, Feig, Jonathan, O'Brien, Edward R, Fisher, Edward A, Moore, Kathryn
    Arterioscler Thromb Vasc Biol, 2013-02-21;33(5):886-93.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Different requirement for Rnd GTPases of R-Ras GAP activity of Plexin-C1 and Plexin-D1.
    Authors: Uesugi K, Oinuma I, Katoh H, Negishi M
    J. Biol. Chem., 2009-01-09;284(11):6743-51.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay

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