Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
5235-S4B-050
Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein Binding Activity
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Citations (2)
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Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 μg/mL, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera binds with an ED50 of 4-24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 4D/CD100 protein
Mouse Semaphorin 4D/CD100
(Phe24-Met711)
Accession # NP_038688.2
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Phe24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass

103 kDa

SDS-PAGE
105-135 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5235-S4B

Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -70 °C under sterile conditions after opening.

Data Image

Binding Activity Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein Binding Activity View Larger

When Recombinant Human Plexin B2 (Catalog # 5329-PB) is coated at 5 µg/mL, 100 µL/well, Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera (Catalog # 5235-S4B) binds with an ED50of 4-24 µg/mL.

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Background: Semaphorin 4D/CD100

Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a 150 kDa type I transmembrane glycoprotein of the Class 4 family of transmembrane immune and nervous system semaphorins (1-3). The mouse Sema4D cDNA encodes 861 amino acids (aa) including a 23 aa signal sequence, a 710 aa extracellular domain (ECD) with sema, PSI and immunoglobulin-like domains, a 21 aa transmembrane domain and a 107 aa cytoplasmic domain. Within the ECD portion expressed (aa 24-711), mouse Sema4D shares 93%, 87%, 82% and 78% aa identity with rat, human, porcine and canine Sema4D, respectively. Sema4D is active as a homodimer that occurs via a membrane-proximal intermolecular disulfide (C707) and hydrophobic interactions involving F244 and F246 (4, 5). Proteolysis by metalloproteinases such as TACE/ADAM17 and MMP-14 (MT1-MMP) produces a soluble, active 120 kDa form that is also active as a dimer (5-7). Sema4D, produced by T cells and activated B and dendritic cells, acts through its low affinity receptor CD72 in the immune system (1, 2). CD72 inhibits the antigen presenting cells that express it; this inhibition is relieved by Sema4D binding (8). Sema4D thus causes enhanced B cell survival, differentiation of macrophages and DC, and enhanced T cell antigen priming (2, 8, 9). Sema4D and its high affinity receptor, Plexin B1, are expressed in complimentary patterns in several regions of the brain, and assist in guidance of developing neurons (10). Lower-affinity Sema4D/Plexin B2 binding also enhances neuronal radial and tangential migration (11). Sema4D produced by tumor-associated macrophages binds endothelial cell Plexin B1 to promote tumor angiogenesis, while Sema4D-induced neovascularization and platelet activation is involved in atherosclerosis (3, 6, 7, 12-14). Receptors associated in cis with plexins, such as Met and ErbB2, can alter Sema4D-mediated activities (3).

References
  1. Furuyama, T. et al. (1996) J. Biol. Chem. 271:33376.
  2. Kumanogoh, A. and H. Kikutani (2004) Cell. Mol. Life Sci. 61:292.
  3. Ch’ng, E.S. and A. Kumanogoh (2010) Mol. Cancer 9:251.
  4. Janssen, B.J.C. et al. (2010) Nature 467:1118.
  5. Elhabazi, A. et al. (2001) J. Immunol. 166:4341.
  6. Zhu, L. et al. (2007) Proc. Natl. Acad. Sci. USA 104:1621.
  7. Basile, J.R. et al. (2007) J. Biol. Chem. 282:6899.
  8. Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
  9. Ishida, I. et al. (2003) Int. Immunol. 15:1027.
  10. Worzfield, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  11. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  12. Sierra, J.R. et al. (2008) J. Exp. Med. 205:1673.
  13. Yukawa, K. et al. (2010) Int. J. Mol. Med. 26:39.
  14. Zhu, L. et al. (2009) Atheroscler. Thromb. Vasc. Biol. 29:1039.
    Entrez Gene IDs
    10507 (Human); 20354 (Mouse); 306790 (Rat)
    Alternate Names
    A8; BB18; CD100 antigen; CD100; CD100M-sema-G; COLL-4; FLJ33485; FLJ34282; FLJ39737; FLJ46484; immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D; MGC169138; MGC169141; M-sema-G; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D; SEMA4D; SEMAJ; Semaphorin 4D; semaphorin-4D

    Citations for Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    2 Citations: Showing 1 - 2
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    1. Adenosine Areceptor (A2AR) stimulation modulates expression of semaphorins 4D and 3A, regulators of bone homeostasis
      Authors: A Mediero, T Wilder, L Shah, BN Cronstein
      FASEB J., 2018;0(0):fj201700217R.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Differentiation
    2. The IkappaB kinase complex is required for plexin-B-mediated activation of RhoA.
      Authors: Zielonka M, Krishnan R, Swiercz J, Offermanns S
      PLoS ONE, 2014;9(8):e105661.
      Species: Mouse
      Sample Types: Whole Cells
      Applications: Bioassay

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