Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF Summary
|Mouse Semaphorin 4D/CD100|
Accession # NP_038688.2
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: Semaphorin 4D/CD100
Semaphorin 4D (Sema4D/CD100, previously sem J, G or C-like 2) is a 150 kDa type I transmembrane glycoprotein of the Class 4 family of transmembrane immune and nervous system semaphorins (1-3). The mouse Sema4D cDNA encodes 861 amino acids (aa) including a 23 aa signal sequence, a 710 aa extracellular domain (ECD) with sema, PSI and immunoglobulin-like domains, a 21 aa transmembrane domain and a 107 aa cytoplasmic domain. Within the ECD portion expressed (aa 24-711), mouse Sema4D shares 93%, 87%, 82% and 78% aa identity with rat, human, porcine and canine Sema4D, respectively. Sema4D is active as a homodimer that occurs via a membrane-proximal intermolecular disulfide (C707) and hydrophobic interactions involving F244 and F246 (4, 5). Proteolysis by metalloproteinases such as TACE/ADAM17 and MMP-14 (MT1-MMP) produces a soluble, active 120 kDa form that is also active as a dimer (5-7). Sema4D, produced by T cells and activated B and dendritic cells, acts through its low affinity receptor CD72 in the immune system (1, 2). CD72 inhibits the antigen presenting cells that express it; this inhibition is relieved by Sema4D binding (8). Sema4D thus causes enhanced B cell survival, differentiation of macrophages and DC, and enhanced T cell antigen priming (2, 8, 9). Sema4D and its high affinity receptor, Plexin B1, are expressed in complimentary patterns in several regions of the brain, and assist in guidance of developing neurons (10). Lower-affinity Sema4D/Plexin B2 binding also enhances neuronal radial and tangential migration (11). Sema4D produced by tumor-associated macrophages binds endothelial cell Plexin B1 to promote tumor angiogenesis, while Sema4D-induced neovascularization and platelet activation is involved in atherosclerosis (3, 6, 7, 12-14). Receptors associated in cis with plexins, such as Met and ErbB2, can alter Sema4D-mediated activities (3).
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- Basile, J.R. et al. (2007) J. Biol. Chem. 282:6899.
- Kumanogoh, A. et al. (2005) Int. Immunol. 17:1277.
- Ishida, I. et al. (2003) Int. Immunol. 15:1027.
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- Zhu, L. et al. (2009) Atheroscler. Thromb. Vasc. Biol. 29:1039.
Citations for Recombinant Mouse Semaphorin 4D/CD100 Fc Chimera Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Adenosine Areceptor (A2AR) stimulation modulates expression of semaphorins 4D and 3A, regulators of bone homeostasis
Authors: A Mediero, T Wilder, L Shah, BN Cronstein
FASEB J., 2018;0(0):fj201700217R.
Sample Types: Whole Cells
The IkappaB kinase complex is required for plexin-B-mediated activation of RhoA.
Authors: Zielonka M, Krishnan R, Swiercz J, Offermanns S
PLoS ONE, 2014;9(8):e105661.
Sample Types: Whole Cells
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