Recombinant Human Plexin B2 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human Plexin B2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human Plexin B2 is coated at 5 μg/mL (100 μL/well), Recombinant Human Semaphorin 4D Fc Chimera (Catalog # 7470-S4) binds with an apparent K<5 nM.
Chinese Hamster Ovary cell line, CHO-derived human Plexin B2 protein
Leu20-Arg1160, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
125.7 kDa
145-180 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Plexin B2

Plexin B2 is a 240 kDa type I transmembrane (TM) glycoprotein of the Plexin B family of semaphorin receptors (1, 2). The human Plexin B2 cDNA encodes 1838 amino acids (aa) that include a 19 aa signal sequence, a 1178 aa extracellular domain (ECD), a 21 aa TM domain, and a 620 aa cytoplasmic region. The ECD contains one semaphorin domain (aa 20-466) and three IPT repeats (aa 803-1092). The ECD may be cleaved into two subunits, a 170 kDa alpha ‑chain (aa 20-1164) and an 80 kDa TM beta ‑chain, that remain noncovalently‑linked (1). Multiple splice variants may exist. Within aa 20-1160 in the ECD, human Plexin B2 shares 82%, 83%, 82% and 81% aa identity with mouse, rat, canine and bovine Plexin B2, respectively. The human B Plexins (B1, B2 and B3) share 39-47% aa identity with each other. Plexin B2 mRNA is expressed in proliferating cerebellar granule cell progenitors, neuroepithelium, developing neurons, growth plate chondrocytes, tooth bud inner enamel epithelium, glomeruli and mesenchyme of the developing kidney, and in germinal center B lymphocytes when T cell help is present (3-7). Plexin B2 is often co‑expressed with Plexin B1, and the two may form heterodimers (1, 4, 6). Genetic deletion of mouse Plexin B2 results in defects in proliferation and migration of cerebellar granule cells, abnormal development of the neural tube and disorganization of the embryonic brain; these defects are not seen when Plexin B1 is deleted (8, 9). Plexin B2 serves as a receptor for type 4 semaphorins, especially Sema4C (8-10). B Plexins, including Plexin B2, can bind the scatter factor receptors, Met and Ron, and activate them upon semaphorin engagement (1, 11).

  1. Artigiani, S. et al. (2003) J. Biol. Chem. 278:10094.
  2. Negishi, M. et al. (2005) Cell. Mol. Life Sci. 62:1363.
  3. Friedel, R.H. et al. (2007) J. Neurosci. 27:3921.
  4. Worzfeld, T. et al. (2004) Eur. J. Neurosci. 19:2622.
  5. Zhang, M. et al. (2008) Bone 43:511.
  6. Perala, N.M. et al. (2005) Gene Expr. Patterns 5:355.
  7. Yu, D. et al. (2008) Immunol. Cell Biol. 86:3.
  8. Deng, S. et al. (2007) J. Neurosci. 27:6333.
  9. Hirschberg, A. et al. (2010) Mol. Cell. Biol. 30:764.
  10. Yukawa, K. et al. (2010) Int. J. Mol. Med. 25:225.
  11. Conrotto, P. et al. (2004) Oncogene 23:5131.
Entrez Gene IDs
23654 (Human); 140570 (Mouse); 315217 (Rat)
Alternate Names
MM1; MM1KIAA0315dJ402G11.3; Nbla00445; PLEXB2; Plexin B2; plexin-B2; PLXNB2


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