Recombinant Mouse Semaphorin 4G Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
6504-S4-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Mouse Semaphorin 4G Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit survival of SK‑OV‑3 human ovarian carcinoma cells. The ED50 for this effect is 1-4 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Semaphorin 4G protein
Mouse Semaphorin 4G
(Val18-Met673)
Accession # Q9WUH7
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
100 kDa (monomer)
SDS-PAGE
105-120 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

6504-S4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS and Tween® 20.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: Semaphorin 4G

Semaphorin 4G (Sema4G) is one of the seven known Class 4 transmembrane semaphorin glycoproteins (1). Class 4 semaphorins have multiple roles in cell attraction or repulsion, including development of nerve pathways in the brain, promoting or inhibiting proliferation, and in some cases organizing immune cell interactions. Receptors include transmembrane plexins, but alternate receptors, such as CD72 for Sema4D and TIM-2 for Sema4A, have been identified (2, 3). The 837 amino acid (aa) mouse Sema4G precursor contains a 17 aa signal sequence, a 656 aa extracellular domain (ECD) containing sema and C2-type immunoglobulin domains, a 21 aa transmembrane domain, and a 143 aa cytoplasmic domain with one Ser/Thr phosphorylation site (4). The mouse Sema4G ECD shares 91% and 97% aa sequence identity with human and rat Sema4G, respectively. It also shares approximately 43% aa identity with Sema4C, the most closely related semaphorin, and colocalizes with Sema4C in the cerebellar cortex (5). Both can bind Plexin-B2 and mediate migration of Plexin-B2-expressing cerebellar granule cells (5). Deletion of both Sema4C and Sema4G augments the phenotype produced by deletion of Sema4C alone (5). Sema4G mRNA is also expressed in the peripheral nervous system and in sensory organs such as retina, cochlea, vomeronasal organ and olfactory epithelium (1). In adults, Sema4G mRNA is found in predominantly in the liver, but is also detected in the kidneys and brain (1). Elevated Sema4G mRNA expression is found in mouse kidney during Plasmodium yoelii infection (6). Sema4G is also identified in a screen for genes that are down-regulated in human colorectal cancer (7).

References
  1. Li, H. et al. (1999) Mech. Dev. 87:169.
  2. Halloran, M.C. and M.A. Wolman (2006) Curr. Opin. Cell Biol. 18:533.
  3. Suzuki, K. et al. (2008) Nat. Immunol. 9:17.
  4. SwissProt Accession # Q9WUH7.
  5. Maier, V. et al. (2001) Mol. Cell. Neurosci. 46:419.
  6. Lao, A.O.T. et al. (2001) J. Immunol. 166:1945.
  7. Wang, X. et al. (2008) Hepatogastroenterology 55:2039.
Entrez Gene IDs
57715 (Human); 26456 (Mouse); 361764 (Rat)
Alternate Names
KIAA1619FLJ20590; MGC102867; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4G; SEMA4G; Semaphorin 4G; semaphorin-4G

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