Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF

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Recombinant Mouse Semaphorin 6B Fc Chimera Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones. 5 μg/mL of Recombinant Mouse Semaphorin 6B Fc Chimera causes >50% growth cone collapse in the presence of 10 ng/mL of Recombinant Human beta -NGF (Catalog # 256-GF).
Human embryonic kidney cell, HEK293-derived mouse Semaphorin 6B protein
Mouse Semaphorin 6B
Accession # O54951
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
91 kDa (monomer)
100-115 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Tween® 20.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Semaphorin 6B

Semaphorin 6B is a member of the class 6 subfamily of semaphorins, a large, highly conserved family of signaling molecules that affect multiple processes including axon guidance, cell migration, synaptogenesis, and dendritic spine formation (1). The class 6 semaphorins are type I transmembrane glycoproteins that contain the characteristic extracellular beta propeller-structured N-terminal semaphorin (sema) domain and also an extracellular plexin-semaphorin-integrin (PSI) domain (2, 3). Additionally, the cytoplasmic domain of Semaphorin 6B contains a proline-rich sequence that has been shown to interact with the SH3 domain of the Src signaling protein (4). Mouse Semaphorin 6B has a molecular weight of approximately 120 kDa and is thought to form disulfide-linked homodimers (4). It shares 95% and 99% amino acid (aa) sequence identity with the human and rat orthologs, respectively. In mice, Semaphorin 6B is highly expressed in developing muscle and in subregions of the nervous system, as well as at low levels in most adult tissues (4). It has been shown to mediate repulsion of mouse sympathetic ganglion axons and hippocampal mossy fibers via binding to the transmembrane protein Plexin A4 (5, 6). Semaphorin 6B has also been shown to be highly expressed in human gastric, breast, and glioblastoma cancer cells and is thought to be involved in tumor differentiation and metastasis (7-9).

  1. Pasterkamp, R.J. and R.J. Giger (2009) Curr. Opin. Neurobiol. 19:263.
  2. Neufeld, G. et al. (2005) Front. Biosci. 10:751.
  3. Roney, K. et al. (2013) Protein Cell 4:17.
  4. Eckhardt, F. et al. (1997) Mol. Cell. Neurosci. 9:409.
  5. Suto, F. et al. (2005) J. Neurosci. 25:3628.
  6. Tawarayama, H. et al. (2010) J. Neurosci. 30:7049.
  7. Correa, R.G. et al. (2001) Genomics 73:343.
  8. Murad, H. et al. (2006) Int. J. Oncol. 28:977.
  9. Ge, C. et al. (2013) J. Int. Med. Res. 41:284.
Entrez Gene IDs
10501 (Human); 20359 (Mouse)
Alternate Names
sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B; Sema6B; SEMAN; Semaphorin 6B; semaphorin VIB; semaphorin Z; semaphorin-6B; semaphorin-6Ba; SEMA-VIB; SEMAZ; SEM-SEMA-Y; UNQ1907/PRO4353


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