Recombinant Mouse SEZ6/BSRP-C Protein, CF

R&D Systems | Catalog # 4989-BC

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse SEZ6/BSRP-C Protein (4989-BC)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q7TSK2

Applications

Bioactivity
View all SEZ6/BSRP-C Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse SEZ6/BSRP-C protein
Gly19-Asn902, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE with silver staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gly19

Predicted Molecular Mass

97 kDa

SDS-PAGE

140-190 kDa, reducing conditions

Activity

Measured by its ability to induce cell clumping and inhibit neurite outgrowth of Neuro‑2A mouse neuroblastoma cells.
2 μg/mL of Recombinant Mouse SEZ6/BSRP-C is able to signifcantly induce cell clumping and inhibit neurite outgrowth.

Formulation, Preparation, and Storage

4989-BC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SEZ6/BSRP-C

Seizure Related Gene 6 (SEZ6), also known as Brain-specific Receptor-like Protein C (BSRP-C), is a member of the SEZ6 family of proteins with a predicted molecular weight of 150 kDa (1, 2). It is a type I transmembrane glycoprotein composed of an extracellular domain that contains three CUB domains and five short consensus repeats (SCRs) (1-3). Alternative splicing generates two additional isoforms for SEZ6. One isoform is a transmembrane protein that shows a deletion of amino acids (aa) 895-907 plus a 10 aa substitution for aa 982-991. The second splice variant is a soluble protein that shows an 18 aa substitution for aa 588-991 (1, 2). Mouse SEZ6 shares 91% and 97% aa sequence identity with the human and rat orthologs, respectively. SEZ6 is expressed in neurons in the developing and adult mouse brain and retina (1-6). It has been suggested that BSRP-C is important for proper development of dendritic arbors and excitatory synapses. SEZ6 has been shown to inhibit neurite branching of cortical neurons with little effect on neurite extension (7). Additionally, SEZ6 has been shown to interact with Motopsin/PRSS12, a serine protease believed to be essential for the reorganization of neuronal structures in the developing and adult brain (8). Mutations in Sez6 have been associated with febrile seizures (9, 10).

References

  1. Shimizu-Nishikawa, K. et al. (1995) Biochem. Biophys. Res. Commun. 216:382.
  2. Miyazaki, T. et al. (2006) FEBS Lett. 580:4057.
  3. Shimizu-Nishikawa, K. et al. (1995) Brain Res. Mol. Brain Res. 28:201.
  4. Kim, M.H. et al. (2002) Mech. Dev. 118:171.
  5. Gunnersen, J.M. et al. (2009) PLoS One 4:e6546.
  6. Osaki, G. et al. (2011) Brain Res. 1386:58.
  7. Gunnersen, J.M. et al. (2007) Neuron 56:621.
  8. Mitsui, S. et al. (2013) Biochem. Biophys. Res. Commun. 436:638.
  9. Yu, Z.L. et al. (2007) J. Neurosci. Res. 85:166.
  10. Mulley, J.C. et al. (2011) Neurol. Res. Int. 2011:917565.

Long Name

Seizure Related 6/Brain-specific Receptor-like Protein-C

Alternate Names

BSRP-C, BSRPC

Entrez Gene IDs

124925 (Human); 20370 (Mouse); 192247 (Rat)

Gene Symbol

SEZ6

UniProt

Q7TSK2

Additional SEZ6/BSRP-C Products

Product Documents for Recombinant Mouse SEZ6/BSRP-C Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse SEZ6/BSRP-C Protein, CF

For research use only

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