Recombinant Mouse Siglec-15 His-tag Protein, CF Summary
Arg24-Tyr250, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse Siglec-15 His-tag (Catalog # 10288-SL) inhibits IL-2 secretion by mouse T cells in the presence of Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841). The ED50 for this effect is 0.075-0.75 µg/mL.
2 μg/lane of Recombinant Mouse Siglec-15 His-tag (Catalog # 10288-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-45 kDa.
Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature mouse Siglec-15 consists of a 239 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 59 aa cytoplasmic domain. Within the ECD, mouse Siglec-15 shares 85% and 92% aa sequence identity with human and rat Siglec-15, respectively. In human Siglec-15, alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6). Siglec-15 is broadly upregulated on human cancer cells and tumor infiltrating myeloid cells (7). Siglec-15 suppresses antigen-specific T cell responses in vitro and in vivo therefore it is considered to be an immune suppressor and potential target for normalization cancer immunotherapy (7).
- Humphrey, M.B. and M.C. Nakamura (2016) Clin. Rev. Allergy Immunol. 51:48.
- Angata, T. et al. (2007) Glycobiology 17:838.
- Takamiya, R. et al. (2013) Glycobiology 23:178.
- Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
- Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
- Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.
- Wang, J. et al. (2019) Nat. med. 25:656.
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