Mouse CD3 Antibody

Catalog # Availability Size / Price Qty
MAB4841-SP
MAB4841-100
MAB4841-500

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CD3 in Mouse Splenocytes.
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Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (4)

Mouse CD3 Antibody Summary

Species Reactivity
Mouse
Specificity
Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
Source
Monoclonal Rat IgG2B Clone # 17A2
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
T cell hybridoma D1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
Mouse splenocytes
Immunohistochemistry
8-25 µg/mL
Perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) and perfusion fixed paraffin-embedded sections of Mouse Thymus
Immunoprecipitation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
 
Complement-dependent Cytotoxicity
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
3-25 µg/mL
See below
T Cell Stimulation
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry CD3 antibody in Mouse Splenocytes by Immunocytochemistry (ICC). View Larger

CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Immunohistochemistry CD3 antibody in Mouse Brain. View Larger

CD3 in Mouse Brain. CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry View Larger

Detection of CD3 in Mouse Thymus. CD3 was detected in perfusion fixed paraffin-embedded sections of Mouse Thymus using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence (A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunocytochemistry/ Immunofluorescence Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence View Larger

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence (A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD3

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

References
  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Entrez Gene IDs
916 (Human); 12501 (Mouse)
Alternate Names
CD_antigen: CD3e; CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3e; CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; IMD18; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE

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Citations for Mouse CD3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Eggerthella lenta augments preclinical autoantibody production and metabolic shift mimicking senescence in arthritis
    Authors: Balakrishnan, B;Luckey, D;Wright, K;Davis, JM;Chen, J;Taneja, V;
    Science advances
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. ITK signaling regulates a switch between T helper 17 and T regulatory cell lineages via a calcium-mediated pathway
    Authors: O Anannya, W Huang, A August
    bioRxiv : the preprint server for biology, 2023-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  3. Dll4 Inhibition Promotes Graft Retention in Fat Grafting Enriched with Adipose-Derived Stem Cells
    Authors: Choong-kun Lee, Bo-Yoon Park, Taehee Jo, Cheol-Heum Park, Ju-Hee Kim, Kyu-Jin Chung et al.
    Stem Cells Translational Medicine
  4. Lack of ApoA-I in ApoEKO Mice Causes Skin Xanthomas, Worsening of Inflammation, and Increased Coronary Atherosclerosis in the Absence of Hyperlipidemia
    Authors: Marco Busnelli, Stefano Manzini, Alice Colombo, Elsa Franchi, Fabrizia Bonacina, Matteo Chiara et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  5. Ephrin receptor A10 monoclonal antibodies and the derived chimeric antigen receptor T cells exert an antitumor response in mouse models of triple-negative breast cancer
    Authors: JH Cha, LC Chan, YN Wang, YY Chu, CH Wang, HH Lee, W Xia, WC Shyu, SP Liu, J Yao, CW Chang, FR Cheng, J Liu, SO Lim, JL Hsu, WH Yang, GN Hortobagyi, C Lin, L Yang, D Yu, LB Jeng, MC Hung
    The Journal of Biological Chemistry, 2022-03-10;0(0):101817.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Generation of functional human thymic cells from induced pluripotent stem cells
    Authors: Stephan A. Ramos, John J. Morton, Prabha Yadav, Brendan Reed, Sheila I. Alizadeh, Ali H. Shilleh et al.
    Journal of Allergy and Clinical Immunology
  7. PD-L1 (Programmed Death Ligand 1) Regulates T-Cell Differentiation to Control Adaptive Venous Remodeling
    Authors: Yutaka Matsubara (松原 裕), Luis Gonzalez, Gathe Kiwan, Jia Liu (刘 佳), John Langford, Mingjie Gao (高 明杰) et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  8. Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?
    Authors: Z Zhang, H Na, Q Gan, Q Tao, Y Alekseyev, J Hu, Z Yan, JB Yang, H Tian, S Zhu, Q Li, IM Rajab, JK Blusztajn, B Wolozin, A Emili, X Zhang, T Stein, LA Potempa, WQ Qiu
    Aging Cell, 2021-10-23;0(0):e13501.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IF
  9. The YAP/HIF-1&alpha/miR-182/EGR2 axis is implicated in asthma severity through the control of Th17 cell differentiation
    Authors: J Zhou, N Zhang, W Zhang, C Lu, F Xu
    Cell & bioscience, 2021-05-12;11(1):84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Itgb3-integrin-deficient mice may not be a sufficient�model for patients with Glanzmann thrombasthenia
    Authors: D Li, J Peng, T Li, Y Liu, M Chen, X Shi
    Molecular Medicine Reports, 2021-04-21;23(6):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Inhibition of T-Cells by Cyclosporine A Reduces Macrophage Accumulation to Regulate Venous Adaptive Remodeling and Increase Arteriovenous Fistula Maturation
    Authors: Yutaka Matsubara (松原裕), Gathe Kiwan, Jia Liu (刘佳), Luis Gonzalez, John Langford, Mingjie Gao (高明杰) et al.
    Arteriosclerosis, Thrombosis, and Vascular Biology
  12. Hepatocyte cannabinoid 1 receptor nullification alleviates toxin-induced liver damage via NF-&kappaB signaling
    Authors: Y Kim, S Gautam, KR Aseer, J Kim, P Chandrasek, CH Mazucanti, P Ghosh, JF O'Connell, ME Doyle, A Appleton, E Lehrmann, QR Liu, JM Egan
    Cell Death & Disease, 2020-12-09;11(12):1044.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Cnp Promoter-Driven Sustained ERK1/2 Activation Increases B-Cell Activation and Suppresses Experimental Autoimmune Encephalomyelitis
    Authors: Marisa A. Jeffries, Alison E. Obr, Kelly Urbanek, Sharyl L. Fyffe-Maricich, Teresa L. Wood
    ASN Neuro
  14. Regulatory T cell phenotype and anti-osteoclastogenic function in experimental periodontitis
    Authors: C Alvarez, S Suliman, R Almarhoumi, ME Vega, C Rojas, G Monasterio, M Galindo, R Vernal, A Kantarci
    Sci Rep, 2020-11-04;10(1):19018.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  15. Modeling human pediatric and adult gliomas in immunocompetent mice through costimulatory blockade
    Authors: Xiaoyan Lan, Dorota A. Kedziorek, Chengyan Chu, Anna Jablonska, Shen Li, Mihoko Kai et al.
    OncoImmunology
  16. Prion protein signaling induces M2 macrophage polarization and protects from lethal influenza infection in mice
    Authors: J Chida, H Hara, K Uchiyama, E Takahashi, H Miyata, H Kosako, Y Tomioka, T Ito, H Horiuchi, H Matsuda, H Kido, S Sakaguchi
    PLoS Pathog., 2020-08-26;16(8):e1008823.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC, Neutralization
  17. Tumor suppressor TET2 promotes cancer immunity and immunotherapy efficacy
    Authors: YP Xu, L Lv, Y Liu, MD Smith, WC Li, XM Tan, M Cheng, Z Li, M Bovino, J Aubé, Y Xiong
    J. Clin. Invest., 2019-07-16;130(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  18. Contributions of T cell dysfunction to the resistance against anti-PD-1 therapy in oral carcinogenesis
    Authors: L Wen, H Lu, Q Li, Q Li, S Wen, D Wang, X Wang, J Fang, J Cui, B Cheng, Z Wang
    J. Exp. Clin. Cancer Res., 2019-07-10;38(1):299.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation
    Authors: Ya-Yuan Fu, Anastasiya Egorova, Catherine Sobieski, Jason Kuttiyara, Marco Calafiore, Shuichiro Takashima et al.
    Immunity
  20. Anti-IL-22 Antibody Attenuates Acute Graft-versus-Host Disease via Increasing Foxp3+ T Cell through Modulation of CD11b+ Cell Function
    Authors: J Wu, J Gu, S Zhou, H Lu, Y Lu, L Lu, X Wang
    J Immunol Res, 2018-08-07;2018(0):1605341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Activation
  21. CXCL10 increases in human skeletal muscle following damage but is not necessary for muscle regeneration
    Authors: MR Deyhle, PS Hafen, J Parmley, CN Preece, M Robison, JR Sorensen, B Jackson, DL Eggett, CR Hancock, RD Hyldahl
    Physiol Rep, 2018-04-01;6(8):e13689.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  22. Nitrosative damage during retrovirus infection-induced neuropathic pain
    Authors: P Chauhan, WS Sheng, S Hu, S Prasad, JR Lokensgard
    J Neuroinflammation, 2018-03-05;15(1):66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Crucial role of OX40/OX40L signaling in a murine model of asthma
    Authors: W Lei, D Zeng, G Liu, Y Zhu, J Wang, H Wu, J Jiang, J Huang
    Mol Med Rep, 2018-01-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  24. A Novel C-Terminal Mutation in Gsdma3 (C+/H-) Leads to Alopecia and Corneal Inflammatory Response in Mice
    Authors: S Swirski, C Röger, A Pienkowska, C Ihlenburg, G Fischer, O May, M Vorm, M Owczarek-L, J Neidhardt
    Invest. Ophthalmol. Vis. Sci., 2018-01-01;59(1):561-571.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  25. Aloperine Protects Mice against DSS-Induced Colitis by PP2A-Mediated PI3K/Akt/mTOR Signaling Suppression
    Authors: X Fu, F Sun, F Wang, J Zhang, B Zheng, J Zhong, T Yue, X Zheng, JF Xu, CY Wang
    Mediators Inflamm., 2017-09-19;2017(0):5706152.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  26. Novel strategies to mimic transmembrane tumor necrosis factor-dependent activation of tumor necrosis factor receptor 2
    Authors: R Fischer, J Marsal, C Guttà, SA Eisler, N Peters, JR Bethea, K Pfizenmaie, RE Kontermann
    Sci Rep, 2017-07-26;7(1):6607.
    Species: Human
    Sample Types: Whole Cells
    Applications: Functional Assay
  27. The excretory-secretory products of Echinococcus granulosus protoscoleces directly regulate the differentiation of B10, B17 and Th17 cells
    Authors: W Pan, WT Hao, YJ Shen, XY Li, YJ Wang, FF Sun, JH Yin, J Zhang, RX Tang, JP Cao, KY Zheng
    Parasit Vectors, 2017-07-21;10(1):348.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  28. Liver-specific deletion of the Plpp3 gene alters plasma lipid composition and worsens atherosclerosis in apoE−/− mice
    Authors: Marco Busnelli, Stefano Manzini, Mika Hilvo, Cinzia Parolini, Giulia S. Ganzetti, Federica Dellera et al.
    Scientific Reports
  29. Characterisation of Immune and Neuroinflammatory Changes Associated with Chemotherapy-Induced Peripheral Neuropathy
    Authors: PG Makker, SS Duffy, JG Lees, CJ Perera, RS Tonkin, O Butovsky, SB Park, D Goldstein, G Moalem-Tay
    PLoS ONE, 2017-01-26;12(1):e0170814.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Selective suppression of the ? isoform of p38 MAPK rescues late-stage tau pathology
    Alzheimers Res Ther, 2016-12-15;8(1):54.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  31. Temporal Expression of Bim Limits the Development of Agonist-Selected Thymocytes and Skews Their TCR? Repertoire
    Authors: David A Hildeman
    J. Immunol., 2016-11-16;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  32. Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
    Authors: Ouyang M, White E, Ren H, Guo Q, Zhang I, Gao H, Yanyan S, Chen X, Weng Y, da Fonseca A, Shah S, Manuel E, Zhang L, Vonderfecht S, Alizadeh D, Berlin J, Badie B
    PLoS ONE, 2016-02-01;11(2):e0148139.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  33. Optimizations of siRNA design for the activation of gene transcription by targeting the TATA-box motif.
    Authors: Fan, Miaomiao, Zhang, Yijun, Huang, Zhuoqion, Liu, Jun, Guo, Xuemin, Zhang, Hui, Luo, Haihua
    PLoS ONE, 2014-09-24;9(9):e108253.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  34. Ninjurin1 deficiency attenuates susceptibility of experimental autoimmune encephalomyelitis in mice.
    Authors: Ahn, Bum Ju, Le, Hoang, Shin, Min Wook, Bae, Sung-Jin, Lee, Eun Ji, Wee, Hee-Jun, Cha, Jong-Ho, Lee, Hyo-Jong, Lee, Hye Shin, Kim, Jeong Hu, Kim, Chang-Ye, Seo, Ji Hae, Lo, Eng H, Jeon, Sejin, Lee, Mi-Ni, Oh, Goo Taeg, Yin, Guo Nan, Ryu, Ji-Kan, Suh, Jun-Kyu, Kim, Kyu-Won
    J Biol Chem, 2013-12-17;289(6):3328-38.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  35. Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
    Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
    Biochem J, 2013-12-15;456(3):463-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  36. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis.
    Authors: Nakamura, Takahiro, Hamuro, Junji, Takaishi, Mikiro, Simmons, Szandor, Maruyama, Kazuichi, Zaffalon, Andrea, Bentley, Adam J, Kawasaki, Satoshi, Nagata-Takaoka, Maho, Fullwood, Nigel J, Itami, Satoshi, Sano, Shigetos, Ishii, Masaru, Barrandon, Yann, Kinoshita, Shigeru
    J Clin Invest, 2013-12-09;124(1):385-97.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. TGF-beta promotes immune responses in the presence of mesenchymal stem cells.
    Authors: Xu C, Yu P, Han X, DU L, Gan J, Wang Y, Shi Y
    J Immunol, 2013-11-29;192(1):103-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  38. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008-10-16;382(1):20-7.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  39. Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
    Authors: Nguyen P, Moisini I, Geiger TL
    Blood, 2003-08-28;102(13):4320-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay

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Mouse CD3 Antibody
By Anonymous on 02/17/2022
Application: IHC Sample Tested: Spleen tissue Species: Mouse

Mouse CD3 Antibody
By Anonymous on 08/13/2021
Application: IHC Sample Tested: Spleen tissue Species: Mouse

Mouse CD3 Antibody
By Anonymous on 06/07/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Paraffined tumor tissue from HCC xenograft Species: Mouse

Green fluorescent is CD3 in mice tumors.


Mouse CD3 Antibody
By Anonymous on 05/16/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Adult heart Species: Mouse

Permeabilisation - 30mins at RT
Block -1% BSA - 30mins at RT
Primary Ab - diluted in block - 1 in 25 - for O/N at 4'C
Secondary ab - diluted in block - 2hrs at RT