Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Complement-dependent Cytotoxicity, T Cell Stimulation, CyTOF-ready

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Neutralization, Flow Cytometry, Immunofluorescence, Immunocytochemistry, Activation, Bioassay, ELISA Capture, Functional Assay

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 17A2
View all CD3 Primary Antibodies »

Product Specifications

Immunogen

T cell hybridoma D1

Specificity

Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse CD3 Antibody

Detection of CD3 in mouse splenocytes by Flow Cytometry

Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) or (B) isotype control antibody (Catalog # MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

CD3 antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

CD3 in Mouse Splenocytes.

CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to plasma membrane. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

CD3 antibody in Mouse Brain.

CD3 in Mouse Brain.

CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Detection of CD3 in Mouse Thymus.

CD3 was detected in perfusion fixed paraffin-embedded sections of Mouse Thymus using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surface of lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

Detection of Mouse CD3 by Immunocytochemistry/Immunofluorescence

(A) T cells (CD3+ cells) were increased at 2 and 7 days postinjury (DPI) compared to 14 DPI (*, P < 0.05) in injured muscles of both wild‐type (WT) and CXCL10 knockout (KO) mice. (B) A representative micrograph of a T‐cell stain (Red = CD3, Blue = DNA) in a WT mouse at 7 DPI. Arrows point out examples of CD3‐positive cells. (C) CXCR3‐positive cells were increased at 2 and 7 DPI compared to 14 DPI (*P < 0.05) in both genotypes. (D) A representative micrograph of CXCR3 (green) stain in a WT mouse at 7 DPI. Arrows mark examples of CXCR3‐positive cells. (E) Approximately 50% of the T cells (CD3 cells) were CXCR3 positive (the CXCL10 receptor). No differences were observed between genotypes or between 2 and 7 DPI. (F) A merged image of panel B and D. The arrow points out an example a cell positive for both CD3 and CXCR3. The circle shows a CD3‐positive, CXCR3‐negative cell. Data are mean ± SD. Scale bar is 100 μm. n = 4–6 per experimental condition. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/29696819), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Mouse CD3 by Immunohistochemistry

Detection of Mouse CD3 by Immunohistochemistry

Peripheral mCRP‐induced neuroinflammation, extravasation of T lymphocytes&AD pathology traits in the ApoE4 brain. (a) We then investigated if mCRP‐induced cerebrovascular damage can migrate peripheral immune cells into the brain. Double immunostaining of CD8 (red), CD3 (green),&double‐positive cells (yellow) was performed to study the transcytosis of T lymphocytes in the cortex after i.p. treatment with PBS (left columns) versus mCRP (right columns) in WT&different ApoE knock‐in mice. Quantification of CD8+ T lymphocytes&CD8+/CD3+ T lymphocytes in the cortex&the comparisons between PBS versus mCRP treatment in each genotype was conducted. mCRP significantly increased the number of T lymphocytes only in the WT (p = 0.05)&ApoE4 (p = 0.001) mice. n = 7–8 in each condition. Image collected & cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34687487), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse CD3 Antibody

Application
Recommended Usage

Complement-dependent Cytotoxicity

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: B220+ Mouse splenocytes

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed mouse splenocytes

Immunohistochemistry

8-25 µg/mL
Sample: Perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) and perfusion fixed paraffin-embedded sections of Mouse Thymus

Immunoprecipitation

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

T Cell Stimulation

Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Reviewed Applications

Read 5 reviews rated 5 using MAB4841 in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CD3

Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.

References

  1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.

Alternate Names

CD_antigen: CD3e, CD3 antigen, delta subunit, CD3d antigen, CD3d antigen, delta polypeptide (TiT3 complex), CD3d molecule, delta (CD3-TCR complex), CD3-DELTA, CD3e, CD3e antigen, CD3e antigen, epsilon polypeptide (TiT3 complex), CD3e molecule, epsilon (CD3-TCR complex), CD3-epsilon, CD3G, CD3g antigen, CD3g antigen, gamma polypeptide (TiT3 complex), CD3g molecule, epsilon (CD3-TCR complex), CD3g molecule, gamma (CD3-TCR complex), CD3-GAMMA, FLJ17620, FLJ17664, FLJ18683, FLJ79544, FLJ94613, IMD18, MGC138597, T3DOKT3, delta chain, T3E, T-cell antigen receptor complex, epsilon subunit of T3, T-cell receptor T3 delta chain, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 delta chain, T-cell surface glycoprotein CD3 epsilon chain, TCRE

Entrez Gene IDs

916 (Human); 12501 (Mouse)

Gene Symbol

CD3E

Additional CD3 Products

Product Documents for Mouse CD3 Antibody

Certificate of Analysis

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Product Specific Notices for Mouse CD3 Antibody

For research use only

Citations for Mouse CD3 Antibody

Customer Reviews for Mouse CD3 Antibody (5)

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Showing  1 - 5 of 5 reviews Showing All
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  • Mouse CD3 Antibody
    Name: HOANG LE
    Application: Immunohistochemistry
    Sample Tested: Spinal cord tissue
    Species: Mouse
    Verified Customer | Posted 10/03/2024
    Mouse CD3 Antibody MAB4841
  • Mouse CD3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 02/17/2022
    Mouse CD3 Antibody MAB4841
  • Mouse CD3 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 08/13/2021
    Mouse CD3 Antibody MAB4841
  • Mouse CD3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Paraffined tumor tissue from HCC xenograft
    Species: Mouse
    Verified Customer | Posted 06/07/2018
    Green fluorescent is CD3 in mice tumors.
    Mouse CD3 Antibody MAB4841
  • Mouse CD3 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Adult heart
    Species: Mouse
    Verified Customer | Posted 05/16/2017
    Permeabilisation - 30mins at RT Block -1% BSA - 30mins at RT Primary Ab - diluted in block - 1 in 25 - for O/N at 4'C Secondary ab - diluted in block - 2hrs at RT
    Mouse CD3 Antibody MAB4841

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