Mouse CD3 Antibody

(6 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
  • Source
    Monoclonal Rat IgG2B Clone # 17A2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    T cell hybridoma D1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • Immunoprecipitation
    Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
  • Complement-dependent Cytotoxicity
    Miescher, G.C. et al. (1989) Immunol. Lett. 23:113 and Wu, L. et al. (1991) J. Exp. Med. 174:1617.
  • Immunocytochemistry
    8-25 µg/mL
    See below
  • T Cell Stimulation
    Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Immunohistochemistry
CD3 in Mouse Brain. CD3 was detected in perfusion fixed HSV-1 infected frozen sections of mouse brain (perivascular cuffing) using 10 µg/mL Rat Anti-Mouse CD3 Monoclonal Antibody (Catalog # MAB4841) overnight at 4 °C. Tissue was stained using the NorthernLights™ 493-conjugated Anti-Rat IgG Secondary Antibody (green; Catalog # NL015) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 antigen, delta subunit; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 6 of 6
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Species
Applications
Sample Type
  1. Metronomic Doses of Temozolomide Enhance the Efficacy of Carbon Nanotube CpG Immunotherapy in an Invasive Glioma Model.
    Authors: Ouyang M, White E, Ren H, Guo Q, Zhang I, Gao H, Yanyan S, Chen X, Weng Y, da Fonseca A, Shah S, Manuel E, Zhang L, Vonderfecht S, Alizadeh D, Berlin J, Badie B
    PLoS ONE, 2016;11(2):e0148139.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC OCT-embedded
  2. LRIG1 inhibits STAT3-dependent inflammation to maintain corneal homeostasis.
    Authors: Nakamura, Takahiro, Hamuro, Junji, Takaishi, Mikiro, Simmons, Szandor, Maruyama, Kazuichi, Zaffalon, Andrea, Bentley, Adam J, Kawasaki, Satoshi, Nagata-Takaoka, Maho, Fullwood, Nigel J, Itami, Satoshi, Sano, Shigetos, Ishii, Masaru, Barrandon, Yann, Kinoshita, Shigeru
    J Clin Invest, 2014;124(1):385-97.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC
  3. TGF-beta promotes immune responses in the presence of mesenchymal stem cells.
    Authors: Xu C, Yu P, Han X, DU L, Gan J, Wang Y, Shi Y
    J Immunol, 2014;192(1):103-9.
    Species: Mouse
    Sample Type: whole cells
    Application: Functional Assay
  4. Regulatory T-cells and cAMP suppress effector T-cells independently of PKA-CREM/ICER: a potential role for Epac.
    Authors: Vang, Amanda G, Housley, William, Dong, Hongli, Basole, Chaitali, Ben-Sasson, Shlomo Z, Kream, Barbara, Epstein, Paul M, Clark, Robert B, Brocke, Stefan
    Biochem J, 2013;456(3):463-73.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
    Authors: De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
    Virology, 2008;382(1):20-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  6. Identification of a murine CD28 dileucine motif that suppresses single-chain chimeric T-cell receptor expression and function.
    Authors: Nguyen P, Moisini I, Geiger TL
    Blood, 2003;102(13):4320-5.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Functional Assay
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