Recombinant Mouse Siglec-G Fc Chimera Protein, CF Summary
Accession # Q80ZE3.1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:|
When Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) is used at 5 μg/mL, Biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.
2 μg/lane of Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 102-114 kDa and 205- 230 kDa, respectively.
Siglecs are type I transmembrane proteins that belong to the immunoglobulin (Ig) superfamily and function as mammalian lectins (1). They are characterized by an extracellular domain consisting of various numbers of Ig domains with a conserved N-terminal V-set Ig ligand-binding domain. This binds species-specific sialic acid motifs on protein and lipid scaffolds to regulate intracellular signaling pathways (2). The cytoplasmic tail has signaling motifs, in most cases immunoreceptor tyrosine-based inhibitory motif (ITIM) (3). Siglec-G is a member of the CD33-related Siglec family in the mouse. It is the apparent ortholog of human Siglec-10 (4). It is expressed by mature B cells, but significant levels of transcript were also detected in DC, myeloid cells, and to a lesser extent, T cells (5). Mature Siglec-G consists of a 526 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 124 aa cytoplasmic domain. Within the ECD, mouse Siglec-G shares 63% and 86% aa sequence identity with the human and rat ortholog Siglec-10, respectively. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (6, 7). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (6). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (8, 9).
- Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
- Poe, J.C. and T.F. Tedder (2012) Trends Immunol. 33:413.
- Meyer, S.J. et al. (2018) Front. Immunol. 9:2820.
- Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
- Chen, G-Y, et al. (2014) Glycobiology 24:800.
- Li, N. et al. (2001) J. Biol. Chem. 276:28106
- Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
- Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
- Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
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