Recombinant Mouse Siglec-G Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10103-SL-050
Recombinant Mouse Siglec-G Fc Chimera Protein, CF Binding Activity
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Recombinant Mouse Siglec-G Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-G Fc Chimera (Catalog # 10103-SL) is used at 5 μg/mL, biotinylated 6'-Sialylactose-PAA binds with an ED50 of 0.2-1.2 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Siglec-G protein
Mouse Siglec-G
(Met19-Lys543)
Accession # Q8OZE3
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Met19
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
86 kDa
SDS-PAGE
102-114 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10103-SL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Mouse Siglec-GFc Chimera (Catalog # 10103-SL) is used at 5 μg/mL, Biotinylated 6'-Sialylactose-PAAbinds with an ED50 of 0.2‑1.2 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Siglec-G Fc Chimera(Catalog # 10103-SL) was resolved with SDS-PAGE under reducing (R) andnon-reducing (NR) conditions and visualized by Coomassie®Blue staining, showing bands at 102-114 kDa and 205- 230 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Siglec-G

Siglecs are type I transmembrane proteins that belong to the immunoglobulin (Ig) superfamily and function as mammalian lectins (1). They are characterized by an extracellular domain consisting of various numbers of Ig domains with a conserved N-terminal V-set Ig ligand-binding domain. This binds species-specific sialic acid motifs on protein and lipid scaffolds to regulate intracellular signaling pathways (2). The cytoplasmic tail has signaling motifs, in most cases immunoreceptor tyrosine-based inhibitory motif (ITIM) (3). Siglec-G is a member of the CD33-related Siglec family in the mouse. It is the apparent ortholog of human Siglec-10 (4). It is expressed by mature B cells, but significant levels of transcript were also detected in DC, myeloid cells, and to a lesser extent, T cells (5). Mature Siglec-G consists of a 526 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 124 aa cytoplasmic domain. Within the ECD, mouse Siglec-G shares 63% and 86% aa sequence identity with the human and rat ortholog Siglec-10, respectively. Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (6, 7). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (6). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (8, 9).

References
  1. Crocker, P.R. et al. (2007) Nat. Rev. Immunol. 7:255.
  2. Poe, J.C. and T.F. Tedder (2012) Trends Immunol. 33:413.
  3. Meyer, S.J. et al. (2018) Front. Immunol. 9:2820.
  4. Angata, T. et al. (2001) J. Biol. Chem. 276:45128.
  5. Chen, G-Y, et al. (2014) Glycobiology 24:800.
  6. Li, N. et al. (2001) J. Biol. Chem. 276:28106
  7. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.
  8. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083.
  9. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355.
Long Name
Sialic Acid Binding Ig-like Lectin G
Entrez Gene IDs
243958 (Mouse)
Alternate Names
Siglec-10; SiglecG; Siglec-G

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