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Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF

Catalog #: 10335-SB Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
10335-SB-050
Recombinant Mouse SIRP beta 1B Fc Chimera Protein Binding Activity
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Summary Product Datasheets Carrier Free Data Images Reconstitution Calculator Background Related Research Areas

Recombinant Mouse SIRP beta 1B Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP beta 1B Fc Chimera (Catalog # 10335-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 40-320 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse SIRP beta 1B protein
Mouse SIRP beta 1B
(Met28-Gly360)
Accession # NP_001166931.1		IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
NP_001166931.1
N-terminal Sequence
Analysis

Met28 and Arg29

Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
64 kDa
SDS-PAGE
70-95 kDa and 175-200 kDa, under reducing conditions

Product Datasheets

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10335-SB

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10335-SB

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse SIRP beta 1B Fc Chimera Protein Binding Activity View Larger

When Recombinant Mouse SIRP beta 1B Fc Chimera (Catalog # 10335-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 40-320 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 1B

Signal-regulatory protein beta 1b (SIRP beta 1b), or CD172b, is a disulfide-linked type I membrane glycoprotein belonging to the SIRP/SHPS/CD172 family of the immunoglobulin (Ig) superfamily. The three SIRP family members are paired receptors that have a conserved extracellular domain (ECD) but differing C-terminal domains and functions (1). The ECD of SIRP beta 1b contains one V-set Ig and two C1-set Ig domains. The mature ECD of mouse SIRP beta 1b shares 57% amino acid sequence identity with human SIRP beta 1. Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages and positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3, 4). SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induces tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or - independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

References
  1. vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. van den Berg, T. et al. (2008) Trends in Immunology 29:203.
  3. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132.
  4. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
  5. Dietrich, J. et al. (2000) J. Immunol. 164:9.
  6. Brook, G. et al. (2004) J. Immunol. 173:2562.
  7. Hayashi, A. et al. (2004) J. Biol. Chem. 279:29450.
  8. Fournier B. et al. (2012) J. Biol. Chem. 287:19386.
  9. Seiffert M. et al. (2001) Blood 97:2741.
Long Name
Signal-regulatory Protein beta 1B
Entrez Gene IDs
668101 (Mouse)
Alternate Names
SIRP beta 1B

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