Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF

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Recombinant Mouse Slit1 (aa 1126-1531) Protein SDS-PAGE
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Recombinant Mouse Slit1 (aa 1126-1531) Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit1, immobilized at 1.25-2.5 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
Mouse myeloma cell line, NS0-derived mouse Slit1 protein
Ser1126-Ala1531, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
45 kDa
60-68 kDa, reducing conditions

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Scientific Data

SDS-PAGE Recombinant Mouse Slit1 (aa 1126-1531) Protein SDS-PAGE View Larger

2μg/lane of Recombinant Mouse Slit1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 60 - 68 kDa.

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Background: Slit1

Slit1 is a member of the Slit family of large secreted axon guidance molecules that are ligands for Robo receptors (1, 2). Like other mammalian family members, the 1531 amino acid (aa), ~200 kDa Slit1 contains a 33 aa signal sequence followed by 23 leucine-rich repeats (LRR, aa 34-900) and 9 EGF-like sequences (aa 930-1451) (2). Mammalian Slits also contain a laminin-G domain between EGF6 and EGF7 (aa 1163-1336), and a C-terminal cysteine-rich domain (cysteine knot; aa 1456-1531) (2). Heparin sulfates are required for interaction of Robo with Slit LRR domains (2, 3). The C-terminal fragment (aa 1126-1531) of mouse Slit 1 shares 96% and 99% aa identity with human and rat respectively. Slit1 and Slit2 (or in some cases Slit3) are expressed in complementary locations during development of the optic and olfactory tracts and the forebrain, and appear to work together to mediate Robo guidance of retinal, olfactory, hippocampal and motor axons (1, 4-9). Deletion of either Slit1 or Slit2 has less effect than deletion of both, which allows axons to wander from tracts and inappropriately cross or recross the midline (4, 5, 7-9). In the injured spinal cord, presence of Slit1 along with Slit3 and Netrin-1 may be responsible for failure of axons to regenerate in the adult CNS (10). Slit1 also promotes dendrite growth and branching of cortical neurons indicating it may exert important influence on the final morphology of cortical neurons (11). Although Slit1 has mainly been found in the fetal and adult brain, it is also detected in the heart and kidney. The C-terminal cysteine knot, which may mediate interaction with other proteins, is absent in the rat brain splicing variant, Slit1 alpha (12).

  1. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  2. Hohenester, E. (2008) Biochem. Soc. Trans. 36:251.
  3. Hussain, S-A. et al. (2006) J. Biol. Chem. 281:39693.
  4. Thompson, H. et al. (2006) Dev. Biol. 296:476.
  5. Plump, A. S. et al. (2002) Neuron 33:219.
  6. Cho, J. H. et al. (2007) J. Neurosci. 27:9094.
  7. Nguyen-Ba-Charvet, K. T. et al. (2002) J. Neurosci. 22:5473.
  8. Bagri, A. et al. (2002) Neuron 33:233.
  9. DiMeglio, T. et al. (2008) J. Neurosci. 28:6285.
  10. Wehrle, R. et al. (2005) Eur. J. Neurosci. 22:2134.
  11. Whitford, K. L. et al. (2002) Neuron 33:47.
  12. Tanno, T. et al. (2004) J. Biochem. 136:575.
Long Name
SLIT Homolog 1
Entrez Gene IDs
6585 (Human); 20562 (Mouse)
Alternate Names
KIAA0813; MEGF4; MEGF4slit (Drosophila) homolog 1; MGC164811; Multiple EGF-like domains protein 4; Multiple epidermal growth factor-like domains protein 4; SLIL1; slit homolog 1 (Drosophila); slit homolog 1 protein; Slit1; Slit-1slit1; SLIT3


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