Recombinant Mouse Slit3 (aa 1117-1523) Protein, CF Summary
Leu1117-Ser1523, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse Slit3 Induces Cortical Neurite Outgrowth. A) Untreated E-16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 1.25 μg/ml of Recombinant Mouse Slit3 (Catalog # 9295-SL).
Slit3 is an approximately 200 kDa member of the Slit family of large secreted axon guidance molecules that are ligands for ROBO receptors (1, 2). It contains a mitochondrial targeting sequence and may also be retained intracellularly (3). Mature mouse Slit3 consists of 4 cassettes of leucine-rich repeats (LRR) flanked by LRR N-terminal and C-terminal domains, followed by multiple EGF-like domains, a Laminin G-like domain, and a C-terminal cysteine-rich domain (4). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds, and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1, 2, 5). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in Slit function (6). Slit3 is also expressed in the lung, kidney, skeletal muscle, and heart, both during development and postnatally (7-9). Mice with genetically disrupted Slit3 show abnormalities in diaphragm and kidney development (8, 9). Slit3 is additionally expressed by vascular endothelial cells and smooth muscle cells (10). It binds to both ROBO1 and ROBO4, but it is the ROBO4 interaction that mediates Slit3-induced angiogenesis (10). It also can enhance the chemokine-induced migration of monocytes (11) and inhibit tumor cell migration, invasion, and epithelial-mesenchymal transition (12, 13). The related Slit2 protein is cleaved in vivo (at a site conserved in Slit3), and the resulting C-terminal fragment binds Plexin A1 and retains the ability to repel axon migration (5, 14). The corresponding C-terminal fragment of Slit3 is able to bind heparin and neutralize its anti-coagulant activity (15). Within this fragment (aa 1117-1523), mouse Slit3 shares 94% and 97% amino acid identity with human and rat Slit3, respectively.
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