Recombinant Mouse Slit3 (aa 34-1116) Protein, CF

Catalog # Availability Size / Price Qty
9296-SL-050
Recombinant Mouse Slit3 (aa 34-1116)  Protein Bioactivity
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Recombinant Mouse Slit3 (aa 34-1116) Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Recombinant Mouse Slit3, immobilized at 2.5-5 μg/mL on a 96-well plate, is able to significantly enhance neurite outgrowth.
Source
Mouse myeloma cell line, NS0-derived mouse Slit3 protein
Cys34-Val1116, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Cys34
Predicted Molecular Mass
122 kDa
SDS-PAGE
121-153 kDa, reducing conditions

Product Datasheets

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9296-SL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9296-SL

Formulation Supplied as a 0.2 μm filtered solution in Sodium Acetate, NaCl and Ethylene Glycol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Bioactivity Recombinant Mouse Slit3 (aa 34-1116)  Protein Bioactivity View Larger

Recombinant Mouse Slit3 (Catalog # 9296-SL) Induces Cortical Neurite Outgrowth. A) Untreated E16-E18 embryonic rat cortical neurons. B) Neurite outgrowth in E16-E18 embryonic rat cortical neurons treated with 5 μg/ml of Recombinant Mouse Slit3.

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Background: Slit3

Slit3 is an approximately 200 kDa member of the Slit family of large secreted axon guidance molecules that are ligands for ROBO receptors (1, 2). It contains a mitochondrial targeting sequence and may also be retained intracellularly (3). Mature mouse Slit3 consists of 4 cassettes of leucine-rich repeats (LRR) flanked by LRR N-terminal and C-terminal domains, followed by multiple EGF-like domains, a Laminin G-like domain, and a C-terminal cysteine-rich domain (4). During development, Slit3 is expressed in the ventral neural tube, developing sensory organs, limb buds, and developing areas of the limbs in patterns that overlap with but are discrete from Slit1 and Slit2 (1, 2, 5). Axons will not be allowed to recross the floor plate unless all three Slit genes are disrupted, suggesting some overlap in Slit function (6). Slit3 is also expressed in the lung, kidney, skeletal muscle, and heart, both during development and postnatally (7-9). Mice with genetically disrupted Slit3 show abnormalities in diaphragm and kidney development (8, 9). Slit3 is additionally expressed by vascular endothelial cells and smooth muscle cells (10). It binds to both ROBO1 and ROBO4, but it is the ROBO4 interaction that mediates Slit3-induced angiogenesis (10). It also can enhance the chemokine-induced migration of monocytes (11) and inhibit tumor cell migration, invasion, and epithelial-mesenchymal transition (12, 13). The related Slit2 protein is cleaved in vivo (at a site conserved in Slit3), and the resulting C-terminal fragment binds Plexin A1 and retains the ability to repel axon migration (5, 14). The corresponding C-terminal fragment of Slit3 is able to bind heparin and neutralize its anti-coagulant activity (15). Within the N-terminal fragment (aa 34-1116), mouse Slit3 shares 95% and 98% amino acid identity with human and rat Slit3, respectively.

References
  1. Blockus, H. and A. Chedotal (2014) Curr. Opin Neurobiol. 27:82.
  2. Gara, R.K. et al. (2015) Drug Discov. Today 20:156.
  3. Little, M. H. et al. (2001) Am. J. Physiol. Cell Physiol. 281:C486.
  4. Yuan, W. et al. (1999) Dev. Biol. 212:290.
  5. Brose, K. et al. (1999) Cell 96:795.
  6. Long, H. et al. (2004) Neuron 42:213.
  7. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350.
  8. Liu, J. et al. (2003) Mech. Dev. 120:1059.
  9. Yuan, W. et al. (2003) Proc. Natl. Acad. Sci. USA 100:5217.
  10. Zhang, B. et al. (2009) Blood 114:4300.
  11. Geutskens, S.B. et al. (2010) J. Immunol. 185:7691.
  12. Denk, A.E. et al. (2011) Int. J. Mol. Med. 28:721.
  13. Zhang, C. et al. (2015) Oncol. Rep. 34:952.
  14. Delloye-Bourgeois, C. et al. (2015) Nat. Neurosci. 18:36.
  15. Condac, E. et al. (2012) Glycobiology 22:1183.
Long Name
SLIT Homolog 3
Entrez Gene IDs
6586 (Human); 20564 (Mouse); 83467 (Rat)
Alternate Names
KIAA0814; MEGF5; MEGF5Multiple epidermal growth factor-like domains protein 5; Multiple EGF-like domains protein 5; SLIL2; SLIL2FLJ10764; slit homolog 3 (Drosophila); slit homolog 3 protein; SLIT1; slit2; Slit3; Slit-3slit (Drosophila) homolog 3

Citation for Recombinant Mouse Slit3 (aa 34-1116) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The histone deacetylase complex MiDAC regulates a neurodevelopmental gene expression program to control neurite outgrowth
    Authors: B Mondal, H Jin, S Kallappago, Y Sedkov, T Martinez, MF Sentmanat, GJ Poet, C Li, Y Fan, SM Pruett-Mil, HM Herz
    Elife, 2020;9(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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