Recombinant Mouse SLITRK3 His-tag Protein, CF Summary
Thr30-Ser655, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Mouse SLITRK3 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 94-105 kDa.
SLITRK3 (Slit and Trk-like family member 3) is an approximately 120 kDa type I transmembrane member of the SLITRK family of proteins which contain a Slit-like extracellular region and a Trk-like cytoplasmic region (1). The extracellular domain (ECD) of mature mouse SLITRK3 contains 6 leucine rich repeats (LRR), a C‑terminal LRR domain, and 6 more LRR flanked by a pair of N- and C‑terminal LRR domains (2, 3). Within the ECD, mouse SLITRK3 shares 98% and 99.5% amino acid sequence identity with human and rat SLITRK3, respectively. SLITRK3 is expressed in multiple regions of the brain, particularly the cerebral cortex and hippocampus (4). SLITRK3 can suppress neurite outgrowth and promote the formation of inhibitory presynaptic structures (3-6). It localizes post-synaptically and binds to PTP-delta and PTP-sigma on pre-synaptic inhibitory terminals (4, 5). SLITRK3 is up-regulated during the development of gastrointestinal stromal tumors (7).
- Ko, J. (2012) Mol. Cells 34:335.
- Aruga, J. et al. (2003) Gene 315:87.
- Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
- Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
- Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
- Loh, K. H. et al. (2016) Cell 166:1295.
- Wang, C.J. et al. (2015) World J. Gastroenterol. 21:8398.
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