Recombinant Mouse SLITRK4 His-tag Protein, CF

Catalog # Availability Size / Price Qty
1811-SK-050
Recombinant Mouse SLITRK4 His-tag Protein, CF SDS-PAGE
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Recombinant Mouse SLITRK4 His-tag Protein, CF Summary

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate
Source
Mouse myeloma cell line, NS0-derived mouse SLITRK4 protein
Asp19-Ser618, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Asp19
Predicted Molecular Mass
68 kDa
SDS-PAGE
90-100 kDa, reducing conditions

Product Datasheets

Data Image

SDS-PAGE Recombinant Mouse SLITRK4 His-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse SLITRK4 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 90-100 kDa.

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Background: SLITRK4

SLITRK4 is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region (1). Within the extracellular domain (ECD), the mouse SLITRK4 shares 98% amino acid sequence identity with human SLITRK4. The ECD of SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, and 6 more LRRs flanked by a pair of N- and C-terminal LRR domains (2, 3). SLITRK4 is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3, 5). SLITRK genes are also differentially expressed in brain tumors, including astrocytoma, oligodendroglioma, glioblastoma, medulloblastoma, and supratentorial primitive neuroectodermal tumor (PNET) (2).

References
  1. Ko, J. (2012) Mol. Cells 34:335.
  2. Aruga, J. et al. (2003) Gene 315:87.
  3. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  4. Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
Long Name
SLIT and NTRK-like Family, Member 4
Entrez Gene IDs
139065 (Human); 245446 (Mouse)
Alternate Names
DKFZp547M2010; SLIT and NTRK-like family, member 4; SLIT and NTRK-like protein 4; slit and trk like gene 4; SLITRK4

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Primary Antibodies

His Tag Antibody

WB,Simple Western,Flow,AP,CyTOF-ready

Protein Purification Kits

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