Recombinant Mouse SLITRK5 Protein, CF

Catalog # Availability Size / Price Qty
9520-SK-050
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Recombinant Mouse SLITRK5 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly enhance neurite outgrowth when immobilized at 3-6 µg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse SLITRK5 protein
Ile41-Ser664, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ile41
Predicted Molecular Mass
70 kDa
SDS-PAGE
96-113 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9520-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: SLITRK5

SLITRK5 (Slit and Trk-like family member 5; also known as LRR-containing protein 11) is a member of the SLITRK family of proteins (1, 2). This family currently includes six members, all of which contain a slit-like extracellular region and a Trk-like cytoplasmic region (3). Mouse SLITRK5 is a type I transmembrane (TM) protein that is 957 amino acids (aa) in length. It contains a putative 40 aa signal sequence, a 624 aa extracellular domain (ECD), a 21 aa TM segment, and a 272 aa cytoplasmic region. The ECD is characterized by the presence of two leucine-rich domains (LRD) that resemble those found in SLITs. Each LRD  contains seven and eight leucine-rich repeats (LRRs), respectively. Mouse SLITRK5 shares 97% and 99% aa identity with Human and Rat SLITRK5, respectively. The exact function of the LRRs is not known. They are assumed to mediate protein-protein-interactions (4). The cytoplasmic region contains two conserved, potential phosphorylation sites at Tyr833 and Tyr917, plus a TrkA-like tyrosine phosphorylation site (YLxxL) at Tyr945. SLITRK5 is expressed on embryonic neurons of the hippocampus (pyramidal layer) and thalamus (3). SLITRK5 was also shown to be expressed in hematopoietic stem cells, leukemias, and subsets of endothelial cells (5). Loss of SLITRK5 leads to manifestation of OCD-like behaviors in mice. SLITRK-5 was identified as an essential molecule at corticostriatal synapses (6). More recent studies have shown that SLITRK5 mediates BDNF-dependent biological functions through direct interaction with TrkB receptors. SLITRK5 primarily interacts with a trans-synaptic binding partner, protein tyrosine phosphatase δ (PTPδ), but upon BDNF stimulation it shifts to cis-interactions with TrkB (7).

References
  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Nagase, T. et al. (2001) DNA Res. 8:179.
  3. Aruga, J. & K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  4. Enkhbayar, P. et al. (2003) Proteins 54:394.
  5. Milde T. (2007) Leukemia. 21:824.
  6. Shmelkov S.V. (2010) Nat. Med. 16:598.
  7. Song M. (2015) Dev. Cell. 33:690.
Long Name
SLIT and NTRK-like Family, Member 5
Entrez Gene IDs
26050 (Human); 75409 (Mouse); 306152 (Rat)
Alternate Names
bA364G4.2; FLJ58374; KIAA0918; leucine-rich repeat-containing protein 11; LRRC11; SLIT and NTRK-like family, member 5; SLIT and NTRK-like protein 5; SLITRK5

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