Recombinant Mouse SLITRK6 Protein, CF

Catalog # Availability Size / Price Qty
1812-SK-050
Recombinant Mouse SLITRK6 Protein, CF SDS-PAGE
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Recombinant Mouse SLITRK6 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived mouse SLITRK6 protein
Met1-Ser607, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser20
Predicted Molecular Mass
66 kDa
SDS-PAGE
80-90 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1812-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Image

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse SLITRK6 was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 80-90 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLITRK6

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region. Mature mouse SLITRK6 consists of a 587 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, mouse SLITRK6 shares 89% and 95% aa sequence identity with human and rat SLITRK6, respectively. SLITRK6 is strongly expressed in the auditory and vestibular sensory epithelia of the ear (3). Its expression in the inner ear promotes innervation and survival of sensory neurons (3, 4). In the brain, SLITRK6 expression is highly restricted in the thalamus, lateral geniculate nucleus, and absent in the cortex (1, 3). SLITRK6 can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (3, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

References
  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
  3. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
  4. Morlet, T. et al. (2014) Laryngoscope. 124:95.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
  7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.
Long Name
SLIT and NTRK-like Family, Member 6
Entrez Gene IDs
84189 (Human); 239250 (Mouse); 290467 (Rat)
Alternate Names
FLJ22774; MGC119595; MGC119596; MGC119597,4832410J21Rik; SLIT and NTRK-like family, member 6; SLIT and NTRK-like protein 6; slit and trk like gene 6; SLITRK6

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