Recombinant Mouse SorCS2 Protein, CF

SorCS2 is a type I transmembrane glycoprotein receptor of the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). These sorting receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in their specific locations (1 - 3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well-defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4 - 6). Mouse SorCS2 is synthesized as a 1159 amino acid (aa) preproform with a 49 aa signal sequence and a potential furin-type proteolytic processing site at aa 117. This would produce a mature SorCS2 protein of 1042 aa with a 961 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, mouse SorCS2 shares 98%, 89%, 85% and 76% aa identity with rat, human, equine and canine SorCS2, respectively. It also shares ~46% aa identity with the ECD of either SorCS1 or SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).