Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF Summary
Ile17-Phe650, with a C-terminal 10-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in sterile PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Background: SPARC-like 1/SPARCL1
SPARCL1 (Secreted Protein, Acidic and Rich in Cysteines-like 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins (1, 2). SPARCL1 is an anti-adhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver (3, 4). Mouse SPARCL1 contains a 16 amino acid (aa) signal sequence and a 634 aa mature region that contains four domains: a 403 aa N-terminal acidic region, a 23 aa
follistatin-like domain, a 55 aa kazal-like segment and a 148 aa calcium binding domain that contains two EF hand motifs (3, 4). Mouse mature SPARCL1 shares 89%, 67%, 63%, 61%, 60% and 58% aa identity with rat, human, equine, canine, porcine and bovine SPARCL1, respectively. The follistatin-like, kazal-like and
calcium-binding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfide-linked homodimerization or by glycosylation and high acidity (3 - 5). Some truncated forms have been reported. In mouse, a 55 kDa C-terminal fragment is the only form in kidney and represent a portion of SPARCL1 in other tissues (6). In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the full-length form is down-regulated in many epithelial cell-derived tumors (7, 8). SPARCL1 inhibits adhesion and spreading on a variety of substrates (5, 9). It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma (10). In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation (3). SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin (6, 11).
- Framson, P. E. and E. H. Sage (2004) J. Cell. Biochem. 92:679.
- Sullivan, M. M. and E. H. Sage (2004) Int. J. Biochem. Cell Biol. 36:991.
- Girard, J. P. and T. A. Springer (1995) Immunity 2:113.
- Bendik, I. et al. (1998) Cancer Res. 58:626.
- Brekken, R. A. et al. (2004) J. Histochem. Cytochem. 52:735.
- Hambrock, H. O. et al. (2003) J. Biol. Chem. 278:11351.
- Lau, C. P. et al. (2006) J. Pathol. 210:469.
- Isler, S. G. et al. (2001) Int. J. Oncol. 18:521.
- Girard, J. P. and T. A. Springer (1996) J. Biol. Chem. 271:4511.
- Gongidi, V. et al. (2004) Neuron 41:57.
- Sullivan, M. M. et al. (2006) J. Biol. Chem. 281:27621.
Citation for Recombinant Mouse SPARC-like 1/SPARCL1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Hevin/Sparcl1 drives pathological pain through spinal cord astrocyte and NMDA receptor signaling
Authors: G Chen, J Xu, H Luo, X Luo, SK Singh, JJ Ramirez, ML James, JP Mathew, M Berger, C Eroglu, RR Ji
JCI Insight, 2022;7(23):.
Sample Types: In Vivo
Applications: In Vivo
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