Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10202-AR-050
Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF Binding Activity
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Recombinant Mouse TEM8/ANTXR1 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Anthrax Protective Antigen is immobilized at 1.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) that produces 50% of the optimal binding response is 5-30 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TEM8/ANTXR1 protein
Mouse TEM8/ANTXR1
(Glu31-Ser319)
Accession # Q9CZ52
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Glu31
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
60 kDa
SDS-PAGE
62-81 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10202-AR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Anthrax Protective Antigen is immobilized at 1.5 μg/mL, 100 μL/well, Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) binds with an ED50 of 5-30 ng/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TEM8/ANTXR1 Fc Chimera (Catalog # 10202-AR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 62-81 kDa and 125-160 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TEM8/ANTXR1

Anthrax toxin receptor 1 (ANTXR1), also known as Tumor endothelial marker 8 (TEM8), is a glycoprotein of the Anthrax Toxin Receptor family that is expressed by endothelial cells. TEM8/ANTXR1 contains a 289 amino acid (aa) extracellular domain, a 21 aa transmembrane domain, and a 222 aa cytoplasmic domain. Isoforms diverging at the C-termiuns include 564 aa (80‑85 kDa), 368 aa (60 kDa), and potentially secreted isoforms of 330 aa and 297 aa (45 kDa) (1). The extracellular domain shares structural similarity with von Willebrand factor type (vWFA) domains, which are characterized by their interactions with ECM components (2, 3). The extracellular domain is involved in reorganization of cell actin cytoskeleton (2, 3). TEM8/ANTXR1 binds Anthrax Protective Antigen with lesser affinity that Anthrax Receptor 2 and induces toxin internalization (4). TEM8/ANTXR1 has been implicated in tumor angiogenesis, as its expression has been shown to up-regulate in tumor blood vessels and is characterized as a tumor endothelial marker (5). TEM8/ANTXR1 was reported to be an amplifier of Wnt signaling in tumor microenvironment (6). Additionally, TEM8/ANTXR1 serves as the receptor for Seneca Valley virus, an oncolytic picornavirus affecting neuroendocrine cancers (7). Mouse TEM8/ANTXR1 shares 99% and 100% aa identity with human and rat TEM8/ANTXR1, respectively, within the extracellular domain.

References
  1. Bradley, KA. et al. (2001) Nature 414:225.
  2. Hotchkiss, K. et al. (2004). Experimental Cell Research. 305:133.
  3. Whittaker, CA. and Hynes, R. (2002). Mol Biol Cell. 13:3369.
  4. Fu, S. et al. (2010) PLOS One. 5:e11203.
  5. Carson-Walter, EB. et al. (2001). Cancer Res. 18:6649.
  6. Verma, K. et al. (2011) PLOS One. 6:e22334.
  7. Miles, L. et al. (2017). J Clin Invest. 8:2957.
Long Name
Tumor Endothelial Marker 8/Anthrax Toxin Receptor 1
Entrez Gene IDs
84168 (Human); 69538 (Mouse); 362393 (Rat)
Alternate Names
anthrax toxin receptor 1; ANTXR1; ATR2310008J16Rik; FLJ10601; FLJ11298; FLJ21776; TEM8; TEM82810405N18Rik; Tumor endothelial marker 8

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