Recombinant Mouse TIM-2 Protein, CF

Catalog # Availability Size / Price Qty
1885-TM-050
Product Details
Citations (1)
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Recombinant Mouse TIM-2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA.

Immobilized Recombinant Mouse TIM-2 (0.5 μg/mL, 100 μL/well) can bind H-ferritin with an apparent KD <3 nM.

Optimal dilutions should be determined by each laboratory for each application.

Source
Mouse myeloma cell line, NS0-derived mouse TIM-2 protein
Met1-Asn229, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
His22
Predicted Molecular Mass
23.5 kDa
SDS-PAGE
40-60 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1885-TM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: TIM-2

T cell immunoglobulin and mucin domain‑2 (TIM‑2) is an approximately 50 kDa member of the TIM family of immune regulatory proteins. TIMs are type I transmembrane proteins with one Ig‑like V domain and a Ser/Thr‑rich mucin domain in their extracellular regions (1). Mature mouse TIM‑2 consists of a 210 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 53 aa cytoplasmic domain (2). Rat TIM‑2, by contrast, possesses the same structural architecture as mouse but contains an additional 46 aa within the mucin domain. Over comparable regions of the ECD, mouse and rat TIM‑2 share 74% aa sequence identity. The isolated ECD forms dimers and tetramers in solution, and these structures suggest the possibility of TIM‑2/TIM‑2 cis interactions (3). TIM‑2 is expressed on Th2 cells, B cells, oligodendrocytes, hepatocytes, hepatic stellate cells, and epithelial cells lining the bile duct and renal tubules (4 ‑ 9). It is upregulated on Th2 cells during cellular activation and functions as a negative regulator of Th2 cell expansion and immune responses (8 ‑ 11). TIM‑2 binds to ligands expressed on activated B cells, macrophages, dendritic cells, and T cells (8, 12). Its ligation by Semaphorin 4A induces tyrosine phosphorylation in the cytoplasmic domain of TIM‑2 (12). TIM‑2 binding to ligand(s) on hepatocytes inhibits hepatocyte differentiation and proliferation (7). TIM‑2 additionally plays a role in iron homeostasis by serving as an endocytic receptor for the heavy chain of Ferritin (H‑Ferritin) (4, 6).

References
  1. Rodriguez-Manzanet, R. et al. (2009) Immunol. Rev. 229:259.
  2. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  3. Santiago, C. et al. (2007) Immunity 26:299.
  4. Todorich, B. et al. (2008) J. Neurochem. 107:1495.
  5. Ruddell, R.G. et al. (2009) Hepatology 49:887.
  6. Chen, T.T. et al. (2005) J. Exp. Med. 202:955.
  7. Watanabe, N. et al. (2007) Hepatology 45:1240.
  8. Chakravarti, S. et al. (2005) J. Exp. Med. 202:437.
  9. Rennert, P.D. et al. (2006) J. Immunol. 177:4311.
  10. Fukushima, A. et al. (2007) Immunol. Lett. 110:133.
  11. Knickelbein, J.E. et al. (2006) J. Immunol. 177:4966.
  12. Kumanogoh, A. et al. (2002) Nature 419:629.
Long Name
T Cell Immunoglobulin Mucin-2
Entrez Gene IDs
171284 (Mouse)
Alternate Names
T Cell immunoglobulin and mucin domain protein 2; TIM2; TIM-2

Citation for Recombinant Mouse TIM-2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A novel affinity-based method for the isolation of highly purified extracellular vesicles
    Sci Rep, 2016;6(0):33935.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Bioassay

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