TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.
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Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
R&D Systems | Catalog # 462-TEC
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Key Product Details
- R&D Systems E. coli-derived Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) (462-TEC)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Bioactivity
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Product Specifications
Source
E. coli-derived mouse TRANCE/TNFSF11/RANK L protein
Lys158-Asp316, with an N-terminal Met
Lys158-Asp316, with an N-terminal Met
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
19 kDa
Activity
Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells.
The ED50 for this effect is 0.5‑2 ng/mL.
The ED50 for this effect is 0.5‑2 ng/mL.
Reviewed Applications
Read 14 reviews rated 4.8 using 462-TEC in the following applications:
- Cell Adhesion (1 Review)
- cell differentiation (1 Review)
- Cell Proliferation (1 Review)
- Immunohistochemistry (1 Review)
- In vitro bioactivity in cell culture (6 Reviews)
- MassSpec characterization. (1 Review)
- Media additive for protein or antibody production (1 Review)
- Stem/Immune cell maintenance or differentiation (2 Reviews)
Scientific Data Images for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) Bioactivity
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 0.5-2 ng/mL.
Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) SDS-PAGE
1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 462-TEC
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 462-TEC/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Calculators
Background: TRANCE/TNFSF11/RANK L
References
- Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
- Anderson, D.M. et al. (1997) Nature 390:175.
- Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
- Kong, Y-Y. et al. (1999) Nature 397:315.
Long Name
TNF-related Activation-induced Cytokine
Alternate Names
CD254, ODF, OPGL, RANK L, RANKL, TNFSF11
Gene Symbol
TNFSF11
UniProt
Additional TRANCE/TNFSF11/RANK L Products
Product Documents for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
For research use only
Related Research Areas
Citations for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed)
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Customer Reviews for Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli-expressed) (14)
4.8 out of 5
14 Customer Ratings
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 04/21/2022
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 12/31/2021
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Application: ImmunohistochemistryVerified Customer | Posted 11/23/2021
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 11/04/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 03/20/2021
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Application: Cell ProliferationVerified Customer | Posted 03/11/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 02/18/2021
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 10/25/2020
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Application: In vitro bioactivity in cell cultureVerified Customer | Posted 06/25/2020
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Application: cell differentiationVerified Customer | Posted 02/21/2020
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Application: MassSpec characterization.Verified Customer | Posted 01/08/2019
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Application: Cell AdhesionVerified Customer | Posted 01/03/2019Bone marrow macrophages grown in media on osteoassay plates for 7 days then treated with 35ng/mL Rank L. After 14 days TRAP stained.
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Application: Media additive for protein or antibody productionVerified Customer | Posted 05/16/2016
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Application: Stem/Immune cell maintenance or differentiationVerified Customer | Posted 01/18/2016
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Associated Pathways
