Recombinant Mouse TRANCE/RANK L/TNFSF11 (E. coli), CF

(20 citations)   
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce osteoclast differentiation of RAW 264.7 mouse monocyte/macrophage cells. The ED50 for this effect is 0.5‑2 ng/mL.
  • Source
    E. coli-derived Lys158-Asp316, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    19 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
462-TEC/CF
 
462-TEC
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA.
Formulation Lyophilized from a 0.2 μm filtered solution in NaH2PO4, NaCl and EDTA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse TRANCE/TNFSF11/RANK L (Catalog # 462-TEC/CF) induces osteoclast differentiation of the RAW 264.7 mouse monocyte/macrophage cell line. The ED50 for this effect is 0.5-2 ng/mL.
1 μg/lane of Recombinant Mouse TRANCE/TNFSF11/RANK L was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 19 kDa.
Background: TRANCE/TNFSF11/RANK L

TRANCE (receptor activator of NF-kappa B ligand [RANK L], also called TNF-related activation-induced cytokine [TRANCE], osteoprotegrin ligand [OPGL], and osteoclast differentiation factor [ODF]), is a member of the tumor necrosis factor (TNF) family. TRANCE was originally identified as an immediate early gene up-regulated by T cell receptor stimulation. The mouse TRANCE cDNA encodes a type II transmembrane protein of 316 amino acids with a predicted cytoplasmic domain of 48 amino acids and an extracellular domain of 247 amino acids. The extracellular domain contains two potential N-linked glycosylation sites. Mouse and human TRANCE share 85% amino acid identity. TRANCE is primarily expressed in T cells and T cell rich organs, such as thymus and lymph nodes. The multi-functions of TRANCE include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function, induction of osteoclastogenesis, and lymph node organogenesis. RANK is the cell surface signaling receptor of TRANCE. RANK has been shown to undergo receptor clustering during signal transduction. Osteoprotegrin, a soluble member of the TNF receptor family which binds TRANCE, is a naturally occurring decoy receptor that counterbalances the effects of TRANCE.

  • References:
    1. Wong, B.R. et al. (1997) J. Biol. Chem. 272:25190.
    2. Anderson, D.M. et al. (1997) Nature 390:175.
    3. Nakagawa, N. et al. (1998) Biochem. Biophys. Res. Commun. 245:382.
    4. Kong, Y-Y. et al. (1999) Nature 397:315.
  • Long Name:
    TNF-related Activation-induced Cytokine
  • Entrez Gene IDs:
    8600 (Human); 21943 (Mouse); 117516 (Rat)
  • Alternate Names:
    CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. SNX10 gene mutation leading to osteopetrosis with dysfunctional osteoclasts
    Authors: EL Stattin, P Henning, J Klar, E McDermott, C Stecksen-B, PE Sandström, TG Kellgren, P Rydén, G Hallmans, T Lönnerholm, A Ameur, MH Helfrich, FP Coxon, N Dahl, J Wikström, UH Lerner
    Sci Rep, 2017;7(1):3012.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. Inhibition of lipopolysaccharide-induced osteoclast formation and bone resorption in vitro and in vivo by cysteine proteinase inhibitors
    Authors: F Strålberg, A Kassem, F Kasprzykow, M Abrahamson, A Grubb, C Lindholm, UH Lerner
    J. Leukoc. Biol, 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  3. Tumor Cell-Derived Exosomes from the Prostate Cancer Cell Line TRAMP-C1 Impair Osteoclast Formation and Differentiation
    PLoS ONE, 2016;11(11):e0166284.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Prader-Willi Critical Region, a Non-Translated, Imprinted Central Regulator of Bone Mass: Possible Role in Skeletal Abnormalities in Prader-Willi Syndrome.
    Authors: Khor E, Fanshawe B, Qi Y, Zolotukhin S, Kulkarni R, Enriquez R, Purtell L, Lee N, Wee N, Croucher P, Campbell L, Herzog H, Baldock P
    PLoS ONE, 2016;11(1):e0148155.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Netrin-1 is a critical autocrine/paracrine factor for osteoclast differentiation.
    Authors: Mediero A, Ramkhelawon B, Perez-Aso M, Moore K, Cronstein B
    J Bone Miner Res, 2015;30(5):837-54.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY.
    Authors: Beier E, Sheu T, Dang D, Holz J, Ubayawardena R, Babij P, Puzas J
    J Biol Chem, 2015;290(29):18216-26.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  7. Activin A inhibits RANKL-mediated osteoclast formation, movement and function in murine bone marrow macrophage cultures.
    Authors: Fowler T, Kamalakar A, Akel N, Kurten R, Suva L, Gaddy D
    J Cell Sci, 2015;128(4):683-94.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation.
    Authors: Sun Q, Sammut B, Wang F, Kurihara N, Windle J, Roodman G, Galson D
    J Bone Miner Res, 2014;29(1):90-102.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  9. NOTCH inhibits osteoblast formation in inflammatory arthritis via noncanonical NF-kappaB.
    Authors: Zhang H, Hilton M, Anolik J, Welle S, Zhao C, Yao Z, Li X, Wang Z, Boyce B, Xing L
    J Clin Invest, 2014;124(7):3200-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. Chloroquine reduces osteoclastogenesis in murine osteoporosis by preventing TRAF3 degradation.
    Authors: Xiu Y, Xu H, Zhao C, Li J, Morita Y, Yao Z, Xing L, Boyce B
    J Clin Invest, 2014;124(1):297-310.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Notch pathway inhibition controls myeloma bone disease in the murine MOPC315.BM model.
    Authors: Schwarzer R, Nickel N, Godau J, Willie B, Duda G, Schwarzer R, Cirovic B, Leutz A, Manz R, Bogen B, Dorken B, Jundt F
    Blood Cancer J, 2014;4(0):e217.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. A nonapoptotic role for CASP2/caspase 2: modulation of autophagy.
    Authors: Tiwari, Meenaksh, Sharma, Lokendra, Vanegas, Difernan, Callaway, Danielle, Bai, Yidong, Lechleiter, James D, Herman, Brian
    Autophagy, 2014;10(6):1054-70.
  13. Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.
    Authors: Muruganandan S, Dranse H, Rourke J, McMullen N, Sinal C
    Stem Cells, 2013;31(10):2172-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  14. Osteoblast CFTR inactivation reduces differentiation and osteoprotegerin expression in a mouse model of cystic fibrosis-related bone disease.
    Authors: Stalvey M, Clines K, Havasi V, McKibbin C, Dunn L, Chung W, Clines G
    PLoS ONE, 2013;8(11):e80098.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Macrophage infiltration predicts a poor prognosis for human ewing sarcoma.
    Authors: Fujiwara T, Fukushi J, Yamamoto S, Matsumoto Y, Setsu N, Oda Y, Yamada H, Okada S, Watari K, Ono M, Kuwano M, Kamura S, Iida K, Okada Y, Koga M, Iwamoto Y
    Am. J. Pathol., 2011;179(3):1157-70.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Inhibition of osteoclastogenesis by mechanically loaded osteocytes: involvement of MEPE.
    Authors: Kulkarni RN, Bakker AD, Everts V, Klein-Nulend J
    Calcif. Tissue Int., 2010;87(5):461-8.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  17. Estrogen-dependent and C-C chemokine receptor-2-dependent pathways determine osteoclast behavior in osteoporosis.
    Authors: Binder NB, Niederreiter B, Hoffmann O, Stange R, Pap T, Stulnig TM, Mack M, Erben RG, Smolen JS, Redlich K
    Nat. Med., 2009;15(4):417-24.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Hematopoietic stem cell-targeted neonatal gene therapy reverses lethally progressive osteopetrosis in oc/oc mice.
    Authors: Johansson MK, de Vries TJ, Schoenmaker T, Ehinger M, Brun AC, Fasth A, Karlsson S, Everts V, Richter J
    Blood, 2007;109(12):5178-85.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Gingival fibroblasts are better at inhibiting osteoclast formation than periodontal ligament fibroblasts.
    Authors: de Vries TJ, Schoenmaker T, Wattanaroonwong N, van den Hoonaard M, Nieuwenhuijse A, Beertsen W, Everts V
    J. Cell. Biochem., 2006;98(2):370-82.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  20. Recruitment of osteoclast precursors by stromal cell derived factor-1 (SDF-1) in giant cell tumor of bone.
    Authors: Liao TS, Yurgelun MB, Chang SS, Zhang HZ, Murakami K, Blaine TA, Parisien MV, Kim W, Winchester RJ, Lee FY
    J. Orthop. Res., 2005;23(1):203-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 20 Citations

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