Recombinant Mouse TrkC Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
10267-TC-050
Recombinant Mouse TrkC Fc Chimera Protein, CF Bioactivity
2 Images
Product Details
FAQs
Reviews

Recombinant Mouse TrkC Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit NT-3-induced proliferation of BaF3 mouse pro‑B cells transfected with human TrkB. The ED50 for this effect is 0.05-0.3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse TrkC protein
Mouse TrkC
(Cys32-Thr429)
Accession # Q6VNS1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Cys32
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
72 kDa
SDS-PAGE
100-125 kDa, under reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10267-TC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse TrkC Fc Chimera (Catalog # 10267-TC) inhibits NT-3-induced proliferation of BaF3 mouse pro-B cells transfected with human TrkB. The ED50 for this effect is 0.05-0.3 μg/mL in the presence of 40 ng/mL of Recombinant Human NT-3 (Catalog # 267-N3).

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse TrkC Fc Chimera (Catalog # 10267-TC) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-125 kDa and 200-250 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: TrkC

Tropomyocin receptor kinase C (TrkC), also named Neurotrophic tyrosine kinase receptor type 1 (NTRK3) is a member of a nerve growth factor tyrosine kinase receptor family. There are three members of the Trk family, TrkA, TrkB and TrkC, and they bind a group of structurally related, secreted proteins termed neurotrophins, which play an important role in the development and function of the nervous system. The Trk family shares a conserved structural motif consisting of two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, a single transmembrane domain and an intracellular tyrosine kinase domain (3). Natural splice variants of the different Trks, lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described (4). Mature mouse TrkC consists of a 398 amino acid (aa) extracellular domain (ECD) which shares 94 % and 100 % aa identity with human and rat TrkC, respectively. Each Trk family member exhibits different ligand specificities: TrkA binds NGF and NT-3, TrkB binds BDNF, NT-3 and NT-4/5, and TrkC only binds NT-3 (1, 2). The biological activities of the neurotrophins are mediated by binding to and activating two unrelated receptor types: the p75 neurotrophin receptor (p75NTR) and the Trk family of receptors (1, 2). P75NTR is a member of the tumor necrosis factor receptor superfamily (TNFRSF) and has been designated TNFRSF16. It binds all neurotrophins with low-affinity to transduce cellular signaling pathways that synergize or antagonize those activated by the Trk receptors. The primary location of TrkC expression is in the nervous system, specifically, in regions of the CNS. Low level TrkC expression has also been observed in a wide variety of tissues outside the nervous system (6). Trk receptor interactions with NGF play major roles in the development of the sympathetic nervous system and are essential for neuronal survival in vivo (8). Increased expression of TrkC has been associated with favorable survival in medulloblastoma patients (8) and inhibition of the Trk receptors may have a range of therapeutic implications (9).

References
  1. Huang, E.J. and L.F. Reichardt. (2003) Annu. Rev. Biochem. 72:609.
  2. Dechant, G. (2001) Cell Tissue Res. 305:229.
  3. Schneider, R. and M. Schweiger (1991) Oncogene 6:1807.
  4. Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019.
  5. Menn, B. et al. (1998) J. Comp. Neurol. 401:47.
  6. Shelton, D. et al. (1995) J. Neurosci. 15:477.
  7. Fagan, A. et al. (1996) J Neurosci. 16(19):6208.
  8. Friedrich, C. et al. (2017) Childs Nerv Syst 33:1463
  9. Yan, W. et al. (2019) J Med. Chem. 62(4):1731.
Long Name
Neurotrophic Tyrosine Kinase Receptor C
Entrez Gene IDs
4916 (Human); 18213 (Mouse); 29613 (Rat)
Alternate Names
EC 2.7.10; EC 2.7.10.1; ETS related protein-neurotrophic receptor tyrosine kinase fusion protein; ETV6-NTRK3 fusion; gp145(trkC); GP145-TrkC; Neurotrophic tyrosine kinase receptor type 3; neurotrophic tyrosine kinase, receptor, type 3; NT 3 receptor; NT-3 growth factor receptor; NTRK3; TrkC tyrosine kinase; TrkC; trk-C; TRKCneurotrophin 3 receptor; tyrosine kinase receptor C

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Mouse TrkC Fc Chimera Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Mouse TrkC Fc Chimera Protein, CF and earn rewards!

Have you used Recombinant Mouse TrkC Fc Chimera Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review