Recombinant Human NT-3 Protein

(11 citations)   
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is typically 1-10 ng/mL.
    The specific activity of Recombinant Human NT-3 is approximately 2082 U/μg, which is calibrated against recombinant human NT-3 Standard (NIBSC code: 98/718).
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived Tyr139-Thr257 (K196R)
  • Accession #
  • N-terminal Sequence
    Analysis
    Tyr139
  • Predicted Molecular Mass
    13.7 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
267-N3
 
267-N3/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute 5 µg vials at 50 µg/mL in sterile PBS. Reconstitute 25 µg or larger vials at 100 µg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Human NT-3 (Catalog #
267‑N3) stimulates cell proliferation in the BaF mouse pro-B cell line transfected with TrkB. The ED50 for this effect is typically
1-10 ng/mL.
1 μg/lane of Recombinant Human NT-3 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.
Background: NT-3

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

  • References:
    1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
    2. Snider, W.D. (1994) Cell 77:627.
    3. Barbacid, M. (1994) J. Neurobiol. 25:1386.
  • Long Name:
    Neurotrophin 3
  • Entrez Gene IDs:
    4908 (Human)
  • Alternate Names:
    3-nucleotidase; NTF3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Showing Results 1 - 10 of 11
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Species
Applications
Sample Type
  1. Modeling the Early Phenotype at the Neuromuscular Junction of Spinal Muscular Atrophy Using Patient-Derived iPSCs.
    Authors: Yoshida M, Kitaoka S, Egawa N, Yamane M, Ikeda R, Tsukita K, Amano N, Watanabe A, Morimoto M, Takahashi J, Hosoi H, Nakahata T, Inoue H, Saito M
    Stem Cell Reports, 2015;4(4):561-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  2. GDF10 is a signal for axonal sprouting and functional recovery after stroke.
    Authors: Li S, Nie E, Yin Y, Benowitz L, Tung S, Vinters H, Bahjat F, Stenzel-Poore M, Kawaguchi R, Coppola G, Carmichael S
    Nat Neurosci, 2015;18(12):1737-45.
    Species: Human
    Sample Type: cells
    Application: Neural Differentiation
  3. The phosphorylation status of Ascl1 is a key determinant of neuronal differentiation and maturation in vivo and in vitro.
    Authors: Ali F, Cheng K, Kirwan P, Metcalfe S, Livesey F, Barker R, Philpott A
    Development, 2014;141(11):2216-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  4. AAV1.NT-3 gene therapy for charcot-marie-tooth neuropathy.
    Authors: Sahenk Z, Galloway G, Clark K, Malik V, Rodino-Klapac L, Kaspar B, Chen L, Braganza C, Montgomery C, Mendell J
    Mol Ther, 2014;22(3):511-21.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  5. Involvement of NT3 and P75(NTR) in photoreceptor degeneration following selective Muller cell ablation.
    Authors: Shen W, Zhu L, Lee S, Chung S, Gillies M
    J Neuroinflammation, 2013;10(0):137.
    Species: Mouse
    Sample Type: In Vivo
  6. Derivation of cerebellar neurons from human pluripotent stem cells.
    Authors: Erceg S, Lukovic D, Moreno-Manzano V, Stojkovic M, Bhattacharya S
    Curr Protoc Stem Cell Biol, 2012;20(0):1H.51.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  7. Reduced spiral ganglion neuronal loss by adjunctive neurotrophin-3 in experimental pneumococcal meningitis.
    Authors: Demel C, Hoegen T, Giese A
    J Neuroinflammation, 2011;8(1):7.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  8. EGF and bFGF pre-treatment enhances neural specification and the response to neuronal commitment of MIAMI cells.
    Authors: Delcroix GJ, Curtis KM, Schiller PC, Montero-Menei CN
    Differentiation, 2010;80(4):213-27.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. Neurotrophins modulate monocyte chemotaxis without affecting macrophage function.
    Authors: Samah B, Porcheray F, Gras G
    Clin. Exp. Immunol., 2008;151(3):476-86.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  10. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation.
    Authors: Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP
    Development, 2007;134(18):3327-38.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
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