Recombinant Mouse VSIG2 Fc Chimera Protein, CF Summary
Accession # Q9Z109-1
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSIG2 Fc Chimera (Catalog # 9598‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 0.75-4.5 μg/mL.
V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as CTM or CT-like protein, is a member of the much larger Ig superfamily. Mature mouse VSIG2 is a single-pass type I transmembrane protein with a 220 aa extracellular domain (ECD) and a 63 aa cytoplasmic region linked by a short transmembrane domain. The ECD of VSIG2 contains one Ig-like C2-type (immunoglobulin-like) and one Ig-like V-type (immunoglobulin-like) domain. Mouse VSIG2 ECD shares 85% and 94% identity with human and rat, respectively. In humans, VSIG2 is highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung (1). V-set domains are Ig-like domains resembling the antibody variable domain and are found in diverse protein families from immunoglobulin light and heavy chains to T cell receptors and adhesion molecules (2). Several VSIG molecules, including VSIG1 and VSIG4, can be classified into the junctional adhesion molecule (JAM) family based on the immunoglobulin folds, V‐ and C2‐types, found in the ECD (3, 4). While the function of VSIG2 is currently unknown, based on the structural similarity to JAM molecules, it is most likely a cell adhesion molecule (2-4). R&D Systems in-house testing indicates that VSIG2 inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.
- Chrétien, I. et al. (1998) Eur J Immunol. 28(12):4094.
- Barclay, A.N. (2003) Semin Immunol. 15(4):215.
- Bazzoni, G. Curr Opin (2003) Cell Biol. 15(5):525.
- Zang, X. and Allison, J.P. (2006) J Clin Invest. 116(10):2590.
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