Recombinant Mouse VSIG2 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9598-VS-050
Recombinant Mouse VSIG2 Fc Chimera Protein Bioactivity
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Recombinant Mouse VSIG2 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.75-4.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse VSIG2 protein
Mouse VSIG2
(Val25-Ala244)
Accession # Q9Z109-1
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
58-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9598-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse VSIG2 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse VSIG2 Fc Chimera (Catalog # 9598‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 0.75-4.5 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG2

V-set and immunoglobulin domain-containing protein 2 (VSIG2), also known as CTM or CT-like protein, is a member of the much larger Ig superfamily. Mature mouse VSIG2 is a single-pass type I transmembrane protein with a 220 aa extracellular domain (ECD) and a 63 aa cytoplasmic region linked by a short transmembrane domain. The ECD of VSIG2 contains one Ig-like C2-type (immunoglobulin-like) and one Ig-like V-type (immunoglobulin-like) domain. Mouse VSIG2 ECD shares 85% and 94% identity with human and rat, respectively. In humans, VSIG2 is highly expressed in stomach, colon, prostate, trachea and thyroid glands and weakly in bladder and lung (1). V-set domains are Ig-like domains resembling the antibody variable domain and are found in diverse protein families from immunoglobulin light and heavy chains to T cell receptors and adhesion molecules (2). Several VSIG molecules, including VSIG1 and VSIG4, can be classified into the junctional adhesion molecule (JAM) family based on the immunoglobulin folds, V‐ and C2‐types, found in the ECD (3, 4). While the function of VSIG2 is currently unknown, based on the structural similarity to JAM molecules, it is most likely a cell adhesion molecule (2-4). R&D Systems in-house testing indicates that VSIG2 inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.

Long Name
V-Set and Immunoglobulin Domain Containing 2
Entrez Gene IDs
23584 (Human); 57276 (Mouse); 300520 (Rat)
Alternate Names
2210413P10Rik; Cortical thymocyte-like protein; CTH; CTHCTXLcortical thymocyte receptor (X. laevis CTX) like; CT-like protein; CTXL; V-set and immunoglobulin domain containing 2; V-set and immunoglobulin domain-containing protein 2; VSIG2

Manufacturing Specifications

  1. Chrétien, I. et al. (1998) Eur J Immunol. 28(12):4094.
  2. Barclay, A.N. (2003) Semin Immunol. 15(4):215.
  3. Bazzoni, G. Curr Opin (2003) Cell Biol. 15(5):525.
  4. Zang, X. and Allison, J.P. (2006) J Clin Invest. 116(10):2590.

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