Recombinant Mouse VSIG8 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9204-VS-050
Recombinant Mouse VSIG8 Fc Chimera Protein Bioactivity
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Recombinant Mouse VSIG8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse VSIG8 protein
Mouse VSIG8
(Val22-Gly262)
Accession # Q6P3A4
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val22
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
54 kDa
SDS-PAGE
56-66 kDa, reducing conditions

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9204-VS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9204-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse VSIG8 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse VSIG-8 Fc Chimera (Catalog # 9204-VS) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.

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Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature mouse VSIG8 consists of a 241 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 134 aa cytoplasmic domain. Within the ECD, mouse VSIG8 shares 88% and 95% aa identity with human and rat VSIG8, respectively. Alternative splicing generates an isoform of mouse VSIG8 that lacks most of the first Ig-like domain. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References
  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.
Long Name
V-Set and Immunoglobulin Domain Containing 8
Entrez Gene IDs
391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)
Alternate Names
V-set and immunoglobulin domain containing 8; VSIG8

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