Recombinant Mouse VSIG8 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.5-3 μg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse VSIG8
    (Val22-Gly262)
    Accession # Q6P3A4
    IEGRMDP Mouse IgG2a
    (Glu98-Lys330)
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Val22
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    54 kDa
  • SDS-PAGE
    56-66 kDa, reducing conditions
9204-VS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse VSIG-8 Fc Chimera (Catalog # 9204-VS)inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. TheED50 for this effect is 0.5-3 μg/mL.
Background: VSIG8
VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature mouse VSIG8 consists of a 241 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 134 aa cytoplasmic domain. Within the ECD, mouse VSIG8 shares 88% and 95% aa identity with human and rat VSIG8, respectively. Alternative splicing generates an isoform of mouse VSIG8 that lacks most of the first Ig-like domain. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.
  • References:
    1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
    2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
    3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.
  • Long Name:
    V-Set and Immunoglobulin Domain Containing 8
  • Entrez Gene IDs:
    391123 (Human); 240916 (Mouse); 289236 (Rat)
  • Alternate Names:
    V-set and immunoglobulin domain containing 8; VSIG8

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