Recombinant Rat CD27/TNFRSF7 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
7888-CD-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Rat CD27/TNFRSF7 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit the proliferation of mouse T cells induced by CD27 Ligand and anti-CD3. The ED50 for this effect is 0.4-1.6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat CD27/TNFRSF7 protein
Rat CD27/TNFRSF7
(Thr21-Arg183)
Accession # NP_001019506
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Thr21
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
45.1 kDa (monomer)
SDS-PAGE
60-66 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

7888-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: CD27/TNFRSF7

CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival (1). Mature rat CD27 consists of a 163 amino acid (aa) extracellular domain (ECD) with three TNFR cysteine‑rich repeats, a 21 aa transmembrane segment, and a 47 aa cytoplasmic domain. Within the ECD, rat CD27 shares 66% and 85% aa sequence identity with human and mouse CD27, respectively. CD27 is expressed as a disulfide‑linked homodimer that carries N‑linked and O‑linked glycosylation (2, 3). Proteolytic cleavage of CD27 results in the shedding of a 28‑32 kDa fragment of the ECD (3). CD27 is weakly expressed on naïve T cells and NK cells and is up‑regulated upon cell activation (3, 4). It is also up‑regulated on activated germinal center B cells, plasma cells, and a subset of memory B cells (5, 6). CD27 binds to the transmembrane glycoprotein CD27 Ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells (1, 7, 8). This interaction contributes to the activation and survival of CD4+ helper T cells (7‑10), CD8+ effector T cells (11, 12), memory T cells (9), and NK cells (4, 13). Ligation of CD27 on B cells promotes germinal center formation and the expansion and affinity maturation of memory B cell responses (5, 14).

References
  1. Nolte, M.A. et al. (2009) Immunol. Rev. 229:216.
  2. van Lier, R.A. et al. (1987) J. Immunol. 139:1589.
  3. Loenen, W.A. et al. (1992) Eur. J. Immunol. 22:447.
  4. Takeda, K. et al. (2000) J. Immunol. 164:1741.
  5. Xiao, Y. et al. (2004) J. Immunol. 172:7432.
  6. Jung, J. et al. (2000) Eur. J. Immunol. 30:2437.
  7. Goodwin, R.G. et al. (1993) Cell 73:447.
  8. Bowman, M.R. et al. (1994) J. Immunol. 152:1756.
  9. Hendriks, J. et al. (2000) Nat. Immunol. 1:433.
  10. van Oosterwijk, M.F. et al. (2007) Int. Immunol. 19:713.
  11. Rowley, T.F. and A. Al Shamkhani (2004) J. Immunol. 172:6039.
  12. Hendriks, J. et al. (2003) J. Exp. Med. 198:1369.
  13. Kelly, J.M. et al. (2002) Nat. Immunol. 3:83.
  14. Raman, V.S. et al. (2003) J. Immunol. 171:5876.
Entrez Gene IDs
939 (Human); 21940 (Mouse); 500318 (Rat); 102139476 (Cynomolgus Monkey)
Alternate Names
CD27 antigen; CD27 molecule; CD27; MGC20393; S152CD27L receptor; T cell activation antigen CD27; T cell activation antigen S152; T14; TNFRSF7; TNFRSF7T-cell activation antigen CD27; Tp55; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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