Recombinant Rat EphA5 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
541-A5-200
Product Details
Citations (1)
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Recombinant Rat EphA5 Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat EphA5 Fc Chimera at 2 µg/mL (100 µL/well) can bind Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2) with a linear range of 0.16-10 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived rat EphA5 protein
Rat EphA5
Ser58-Gln573
(Asp170Glu)
Accession #P54757
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser58
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
84 kDa (monomer)
SDS-PAGE
110 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

541-A5

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

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Background: EphA5

EphA5, also known as Ehk1, Bsk, Cek7, Hek7, and Rek7 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA5 has been shown to bind ephrin-A5, ephrin-A1,
ephrin-A2, ephrin-A3, and ephrin-A4 (2, 3). The extracellular domains of rat EphA5 share 98.5% amino acid identity with the mouse homolog and 96.5% identity with the human homolog. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Entrez Gene IDs
2044 (Human); 13839 (Mouse); 79208 (Rat)
Alternate Names
Bsk; Cek7; EC 2.7.10; EC 2.7.10.1; Ehk1; EHK-1; EHK1Hek7; EK7; EPH homology kinase 1; Eph homology kinase-1; EPH receptor A5; EphA5; EPH-like kinase 7; ephrin type-A receptor 5; Hek7; receptor protein-tyrosine kinase HEK7; Rek7; TYRO4HEK7CEK7; tyrosine-protein kinase receptor EHK-1

Citation for Recombinant Rat EphA5 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Targeting the EphA4 receptor in the nervous system with biologically active peptides.
    Authors: Murai KK, Nguyen LN, Koolpe M, McLennan R, Krull CE, Pasquale EB
    Mol. Cell. Neurosci., 2003;24(4):1000-11.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay

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