Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF

Catalog # Availability Size / Price Qty
9596-CD-100
Recombinant Rat Fc gamma RIIIA/CD16a Protein Bioactivity
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Recombinant Rat Fc gamma RIIIA/CD16a Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Rat Fc gamma  RIIIA/CD16a that produces 50% of the optimal binding response is 0.1-0.6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat Fc gamma RIIIA/CD16a protein
Gly21-Pro200, with a C-ternimal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala20 and Gly21
Predicted Molecular Mass
21 kDa
SDS-PAGE
24-36 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9596-CD

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Rat Fc gamma RIIIA/CD16a Protein Bioactivity View Larger

When Rat IgG is immobilized at 2 μg/mL, 100 μL/well, Recombinant Rat Fc gamma RIIIA/CD16a (Catalog # 9596-CD) binds with an ED50 of 0.1-0.6 μg/mL.

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Reconstitution Calculator

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Background: Fc gamma RIIIA/CD16a

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, cytokine release, and B cell proliferation. There are three classes of Fc gamma Rs, based on close relationships in their extracellular domains: Fc gamma RI/CD64, Fc gamma RII/CD32, and Fc gamma RIII/CD16. Each group may be encoded by multiple genes and exists in different isoforms depending on species and cell type (1-3). Fc gamma RIII has two isoforms, Fc gamma  RIIIA and Fc gamma  RIIIB. Fc gamma  RIII, the only Fc gamma  R found on natural killer cells, is also expressed on macrophages, neutrophils, and mast cells (1-3). Unlike Fc gamma  RI which binds IgG with high affinity, Fc gamma  RIIIA is a low affinity receptor that recognizes IgG as aggregates surrounding multivalent antigens during late immune responses (4, 5). The mature protein consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic domain. Within the ECD, rat Fc gamma  RIIIA shares 83% and 63% amino acid sequence identity with mouse and human Fc gamma  RIIIA, respectively.

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today. 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Chenoweth, A.M. et al. (2015) Immunol. Rev. 268:175.
Long Name
Fc gamma Receptor III A
Entrez Gene IDs
2214 (Human); 304966 (Rat)
Alternate Names
CD16a antigen; CD16a; CD16FCRIIIA; Fc fragment of IgG, low affinity IIIa, receptor (CD16a); Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc gamma receptor III-A; Fc gamma RIIIA; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; FCGR3; FCGR3A; FCGRIII; FcgRIIIA; FcR-10; FCRIII; FcRIIIa; IGFR3FcgammaRIIIA; immunoglobulin G Fc receptor III; low affinity III, receptor for (CD16); low affinity immunoglobulin gamma Fc region receptor III-A; neutrophil-specific antigen NA

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