Recombinant Rat GFR alpha-1 Fc Chimera Protein

Carrier Free

Catalog # Availability Size / Price Qty
560-GR-100/CF

With Carrier

Catalog # Availability Size / Price Qty
560-GR-100
Product Details
Citations (13)
FAQs
Supplemental Products
Reviews (2)

Recombinant Rat GFR alpha-1 Fc Chimera Protein Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to confer GDNF responsiveness to PC‑12 rat adrenal pheochromocytoma cells using a neurite outgrowth assay. Treanor, J. et al. (1996) Nature 392:80. The ED50 for this effect is approximately 0.3 µg/mL in the presence of 25 ng/mL of Recombinant Rat GDNF (Catalog # 512-GF). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GDNF (Catalog # 512-GF) at 1 μg/mL binds Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera with an apparent KD <5 nM.
Source
Mouse myeloma cell line, NS0-derived rat GFR alpha-1/GDNF R alpha-1 protein
Rat GFR alpha -1
(Asp25-Leu445)
Accession # Q62997
CSIEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Asp25
Predicted Molecular Mass
74 kDa (monomer)
SDS-PAGE
95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

560-GR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

560-GR/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-1/GDNF R alpha-1

Glial cell line-derived growth factor (GDNF), neurturin (NTN) and persephin, distant members of the TGF‑ beta  superfamily, are neurotrophic factors for a variety of neuronal populations in the central and peripheral nervous systems. The bioactivities of GDNF and NTN are mediated through a receptor complex composed of the non ligand-binding signaling subunit (c-Ret receptor tyrosine kinase) and either of two ligand binding subunits (GDNF receptor alpha - (GFR alpha -1) or GFR alpha -2). GFR alpha -1 and -2 are members of a family of at least four cysteine‑rich glycosyl-phosphatidylinositol (GPI)‑linked cell surface proteins that share conserved placements of many of their cysteine residues. Binding of GDNF to membrane-associated GFR alpha -1 or GFR alpha -2 initiates the association with and activation of the Ret tyrosine kinase. Soluble GFR alpha s released enzymatically from the cell surface-associated protein with phosphatidylinositol phospholipase C, as well as recombinantly produced soluble GFR alpha -1, can also bind with high-affinity to GDNF and trigger the activation of Ret tyrosine kinase.

Rat GFR alpha -1 cDNA encodes a 468 amino acid (aa) residue protein with an N-terminal 24 aa residue hydrophobic signal peptide. Like other GPI-linked proteins, rat GFR alpha -1 has a C-terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Human GFR alpha -1 shares 93% amino acid identity with rat GFR alpha -1. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors.

References
  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
Entrez Gene IDs
2674 (Human); 14585 (Mouse); 25454 (Rat)
Alternate Names
DKFZp313E1012; DKFZp686J0156; GDNF family receptor alpha 1; GDNF family receptor alpha-1; GDNF R alpha-1; GDNF receptor alpha-1; GDNFRAGFR-ALPHA-1; GDNFR-alpha-1; GDNFRMGC23045; GFR alpha1; GFR alpha-1; GFRA1; GFRa-1; GFR-alpha-1; Glial cell line-derived neurotrophic factor receptor alpha; GPI-linked anchor protein; PI-linked cell-surface accessory protein; RET ligand 1; RET1L; RETL1FLJ10561; TGF-beta related neurotrophic factor receptor 1; TGF-beta-related neurotrophic factor receptor 1; TRNR1FLJ31546

Citations for Recombinant Rat GFR alpha-1 Fc Chimera Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. TGF-?1 promotes expression of fibrosis-related genes through the induction of histone variant H3.3 and histone chaperone HIRA
    Authors: T Shindo, S Doi, A Nakashima, K Sasaki, K Arihiro, T Masaki
    Sci Rep, 2018;8(1):14060.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Gfra1 under-expression causes Hirschsprung's disease and associated enterocolitis in mice
    Authors: LL Porokuokka, HT Virtanen, J Lindén, Y Sidorova, T Danilova, M Lindahl, M Saarma, JO Andressoo
    Cell Mol Gastroenterol Hepatol, 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. DDX4-EGFP transgenic rat model for the study of germline development and spermatogenesis
    Authors: K Gassei, Y Sheng, A Fayomi, P Mital, M Sukhwani, CC Lin, KA Peters, A Althouse, H Valli, KE Orwig
    Biol. Reprod., 2017;96(3):707-719.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  4. Curcumin inhibits glial scar formation by suppressing astrocyte-induced inflammation and fibrosis in vitro and in vivo
    Authors: Jiangkai Lin
    Brain Res., 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Glial-cell-derived neuroregulators control type 3 innate lymphoid cells and gut defence
    Nature, 2016;535(7612):440-3.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  6. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014;514(7520):98-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
    Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
    Mol Cell Neurosci, 2013;54(0):30-43.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  8. Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases.
    Authors: Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu
    J Biol Chem, 2013;288(32):23421-31.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Glial cell line-derived neurotrophic factor and endothelial cells promote self-renewal of rabbit germ cells with spermatogonial stem cell properties.
    Authors: Kubota H, Wu X, Goodyear SM, Avarbock MR, Brinster RL
    FASEB J., 2011;25(0):2604-14.
    Species: Rabbit
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
    Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
    Biochemistry, 2005;44(7):2545-54.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: ELISA Developmet
  11. The neuronal scaffold protein Shank3 mediates signaling and biological function of the receptor tyrosine kinase Ret in epithelial cells.
    Authors: Schuetz G, Rosario M, Grimm J, Boeckers TM, Gundelfinger ED, Birchmeier W
    J. Cell Biol., 2004;167(5):945-52.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. The soluble ectodomain of RetC634Y inhibits both the wild-type and the constitutively active Ret.
    Authors: Cerchia L, Libri D, Carlomagno MS, de Franciscis V
    Biochem. J., 2003;372(0):897-903.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  13. TGFbeta induces GDNF responsiveness in neurons by recruitment of GFRalpha1 to the plasma membrane.
    Authors: Peterziel H, Unsicker K, Krieglstein K
    J. Cell Biol., 2002;159(1):157-67.
    Species: Chicken
    Sample Types: Whole Cells
    Applications: Bioassay

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Reviews for Recombinant Rat GFR alpha-1 Fc Chimera Protein

Average Rating: 4.5 (Based on 2 Reviews)

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Recombinant Rat GFR alpha-1 Fc Chimera Protein
By Anonymous on 05/19/2017
Application: Stem/Immune cell maintenance or differentiation
Reason for Rating: Works well for our purposes (growing spermatogonial stem cells)

Recombinant Rat GFR alpha-1 Fc Chimera Protein
By Anonymous on 08/01/2016
Application: Stem/Immune cell maintenance or differentiation