Recombinant Rat GDNF Protein

Carrier Free

Catalog # Availability Size / Price Qty
512-GF-010/CF
512-GF-050/CF

With Carrier

Catalog # Availability Size / Price Qty
512-GF-010
512-GF-050
512-GF-01M
Recombinant Rat GDNF Protein Bioactivity
2 Images
Product Details
Citations (29)
FAQs
Supplemental Products
Reviews (3)

Recombinant Rat GDNF Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha ‑1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived rat GDNF protein
Ser78-Ile211
Accession #
N-terminal Sequence
Analysis
Ser78
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
15 kDa (monomer)

Product Datasheets

512-GF (with carrier)

512-GF/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

512-GF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

512-GF/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Rat GDNF Protein Bioactivity View Larger

Recombinant Rat GDNF (Catalog # 512‑GF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.

SDS-PAGE Recombinant Rat GDNF Protein SDS-PAGE View Larger

1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GDNF

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Long Name
Glial Cell line-derived Growth Factor
Entrez Gene IDs
2668 (Human); 25453 (Rat)
Alternate Names
Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; HGDNF; HSCR3

Citations for Recombinant Rat GDNF Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Motoneuron expression profiling identifies an association between an axonal splice variant of HDGF-related protein 3 and peripheral myelination
    Authors: BE Kerman, S Genoud, B Kurt Vatan, AM Denli, SG Ghosh, X Xu, GW Yeo, JB Aimone, FH Gage
    J. Biol. Chem., 2020;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Protocol Optimization for the Production of the Non-Cytotoxic J&deltaNI5 HSV Vector Deficient in Expression of Immediately Early Genes
    Authors: S Kuroda, Y Miyagawa, Y Sato, M Yamamoto, K Adachi, H Kinoh, WF Goins, JB Cohen, JC Glorioso, N Taniai, H Yoshida, T Okada
    Mol Ther Methods Clin Dev, 2020;17(0):612-621.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Biological and RNA regulatory function of MOV10 in mammalian germ cells
    Authors: K Fu, S Tian, H Tan, C Wang, H Wang, M Wang, Y Wang, Z Chen, Y Wang, Q Yue, Q Xu, S Zhang, H Li, J Xie, M Lin, M Luo, F Chen, L Ye, K Zheng
    BMC Biol., 2019;17(1):39.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis
    Authors: C Wu, ME Watts, LL Rubin
    Cell Rep, 2019;26(5):1143-1156.e5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Neuronal subtype determines HSV-1 Latency-Associated-Transcript (LAT) promoter activity during latency
    Authors: JR Cabrera, AJ Charron, DA Leib
    J. Virol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS
    Authors: KL Gibbs, B Kalmar, ER Rhymes, AD Fellows, M Ahmed, P Whiting, CH Davies, L Greensmith, G Schiavo
    Cell Death Dis, 2018;9(6):596.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Previously claimed male germline stem cells from porcine testis are actually progenitor Leydig cells
    Authors: Y Bai, C Zhu, M Feng, H Wei, L Li, X Tian, Z Zhao, S Liu, N Ma, X Zhang, R Shi, C Fu, Z Wu, S Zhang
    Stem Cell Res Ther, 2018;9(1):200.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  8. DNA methylation of the Fthl17 5'-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells
    Authors: N Aoki, K Mochizuki, Y Matsui
    PLoS ONE, 2017;12(2):e0172219.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells
    Authors: A Suzuki, M Hirasaki, T Hishida, J Wu, D Okamura, A Ueda, M Nishimoto, Y Nakachi, Y Mizuno, Y Okazaki, Y Matsui, JC Izpisua Be, A Okuda
    Nat Commun, 2016;7(0):11056.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  10. A system to study mechanisms of neuromuscular junction development and maintenance
    Development, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  11. In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells
    Cell Rep, 2016;17(10):2789-2804.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development.
    Authors: Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G
    PLoS ONE, 2015;10(6):e0129944.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014;514(7520):98-101.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors.
    Authors: Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G
    EMBO J, 2014;33(14):1582-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation.
    Authors: Lim, Jung Jin, Seol, Dong Won, Choi, Kyung He, Shin, Dong Hyu, Kim, Hyung Jo, Song, Seung-Hu, Lee, Dong Ryu
    Sci Rep, 2014;4(0):5923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Bioassay
  16. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
    Authors: Lim J, Shim M, Lee J, Lee D
    PLoS ONE, 2014;9(4):e90454.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  17. Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Authors: Hong, Weimin C, Amara, Susan G
    FASEB J, 2013;27(8):2995-3007.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.
    Authors: Dovere, Lisa, Fera, Stefania, Grasso, Margheri, Lamberti, Dante, Gargioli, Cesare, Muciaccia, Barbara, Lustri, Anna Mar, Stefanini, Mario, Vicini, Elena
    PLoS ONE, 2013;8(4):e59431.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases.
    Authors: Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu
    J Biol Chem, 2013;288(32):23421-31.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
    Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
    Mol Cell Neurosci, 2013;54(0):30-43.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Bioassay
  21. Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose.
    Gene Ther., 2012;19(9):886-98.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  22. Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos.
    Authors: Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A
    Development, 2010;137(5):693-704.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation.
    Authors: He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M
    Stem Cells, 2007;26(1):266-78.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Long-term culture of male germline stem cells from hamster testes.
    Authors: Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T
    Biol. Reprod., 2007;78(4):611-7.
    Species: Hamster
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor.
    Authors: Garbayo E, Ansorena E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ
    Int J Pharm, 2007;344(1):9-15.
    Species: N/A
    Sample Types: N/A
    Applications: Western Blot
  27. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006;281(32):22503-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  28. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
    Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
    Biochemistry, 2005;44(7):2545-54.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  29. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
    Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
    J. Clin. Invest., 2005;115(9):2393-401.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Rat GDNF Protein

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By Anonymous on 03/11/2020
Application: Tested with ELISA before using in a rat model.

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