Recombinant Rat GDNF Protein

(22 citations)
(2 Reviews)
  
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using SH‑SY5Y human neuroblastoma cells. The ED50 for this effect is 2-12 ng/mL in the presence of Recombinant Rat GFR alpha ‑1/GDNF R alpha -1 Fc Chimera (Catalog # 560-GR). Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat GFR alpha ‑1/GDNF R alpha ‑1 Fc Chimera (Catalog # 560-GR) at 1 µg/mL binds Recombinant Rat GDNF with an apparent Kd <4 nM.
  • Source
    Spodoptera frugiperda, Sf 21 (baculovirus)-derived Ser78-Ile211
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser78
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    15 kDa (monomer)
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
512-GF
 
512-GF/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Rat GDNF (Catalog # 512‑GF) promotes the survival of chick embryonic dorsal root ganglia neurons. The ED50 for this effect is 1-3 ng/mL.
1 μg/lane of Recombinant Rat GDNF was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing major bands at 19 kDa and 33 kDa, respectively.
Background: GDNF
Glial Cell Line-derived Neurotrophic Factor (GDNF) is a discovered neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Rat GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein composed of two 134 amino acid residue subunits. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

  • Long Name:
    Glial Cell line-derived Growth Factor
  • Entrez Gene IDs:
    2668 (Human); 25453 (Rat)
  • Alternate Names:
    Astrocyte-derived trophic factor; ATF; ATF1; ATF2; GDNF; glial cell derived neurotrophic factor; glial cell line derived neurotrophic factor; glial cell line-derived neurotrophic factor; HFB1-GDNF; hGDNF; HSCR3
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. DNA methylation of the Fthl17 5'-upstream region regulates differential Fthl17 expression in lung cancer cells and germline stem cells
    Authors: N Aoki, K Mochizuki, Y Matsui
    PLoS ONE, 2017;12(2):e0172219.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  2. A system to study mechanisms of neuromuscular junction development and maintenance
    Development, 2016;0(0):.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  3. Loss of MAX results in meiotic entry in mouse embryonic and germline stem cells
    Authors: A Suzuki, M Hirasaki, T Hishida, J Wu, D Okamura, A Ueda, M Nishimoto, Y Nakachi, Y Mizuno, Y Okazaki, Y Matsui, JC Izpisua Be, A Okuda
    Nat Commun, 2016;7(0):11056.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. In�Vitro Derivation and Propagation of Spermatogonial Stem Cell Activity from Mouse Pluripotent Stem Cells
    Cell Rep, 2016;17(10):2789-2804.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  5. Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development.
    Authors: Schmieg N, Thomas C, Yabe A, Lynch D, Iglesias T, Chakravarty P, Schiavo G
    PLoS ONE, 2015;10(6):e0129944.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  6. Spermatogonial stem cell enrichment using simple grafting of testis and in vitro cultivation.
    Authors: Lim, Jung Jin, Seol, Dong Won, Choi, Kyung He, Shin, Dong Hyu, Kim, Hyung Jo, Song, Seung-Hu, Lee, Dong Ryu
    Sci Rep, 2014;4(0):5923.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  7. Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors.
    Authors: Terenzio M, Golding M, Russell M, Wicher K, Rosewell I, Spencer-Dene B, Ish-Horowicz D, Schiavo G
    EMBO J, 2014;33(14):1582-98.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  8. Three-step method for proliferation and differentiation of human embryonic stem cell (hESC)-derived male germ cells.
    Authors: Lim J, Shim M, Lee J, Lee D
    PLoS ONE, 2014;9(4):e90454.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  9. The neurotrophic factor receptor RET drives haematopoietic stem cell survival and function.
    Authors: Fonseca-Pereira D, Arroz-Madeira S, Rodrigues-Campos M, Barbosa I, Domingues R, Bento T, Almeida A, Ribeiro H, Potocnik A, Enomoto H, Veiga-Fernandes H
    Nature, 2014;514(7520):98-101.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  10. The niche-derived glial cell line-derived neurotrophic factor (GDNF) induces migration of mouse spermatogonial stem/progenitor cells.
    Authors: Dovere, Lisa, Fera, Stefania, Grasso, Margheri, Lamberti, Dante, Gargioli, Cesare, Muciaccia, Barbara, Lustri, Anna Mar, Stefanini, Mario, Vicini, Elena
    PLoS ONE, 2013;8(4):e59431.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  11. Differential targeting of the dopamine transporter to recycling or degradative pathways during amphetamine- or PKC-regulated endocytosis in dopamine neurons.
    Authors: Hong, Weimin C, Amara, Susan G
    FASEB J, 2013;27(8):2995-3007.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  12. Substrate specificity of R3 receptor-like protein-tyrosine phosphatase subfamily toward receptor protein-tyrosine kinases.
    Authors: Sakuraba, Juichi, Shintani, Takafumi, Tani, Sachiko, Noda, Masaharu
    J Biol Chem, 2013;288(32):23421-31.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  13. Glial cell line-derived neurotrophic factor (GDNF) induces neuritogenesis in the cochlear spiral ganglion via neural cell adhesion molecule (NCAM).
    Authors: Euteneuer S, Yang K, Chavez E, Leichtle A, Loers G, Olshansky A, Pak K, Schachner M, Ryan A
    Mol Cell Neurosci, 2013;54(0):30-43.
    Species: Rat
    Sample Type: Whole Tissue
    Application: Bioassay
  14. Non-viral gene therapy for GDNF production in RCS rat: the crucial role of the plasmid dose.
    Gene Ther., 2012;19(9):886-98.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  15. Isolation and propagation of enteric neural crest progenitor cells from mouse embryonic stem cells and embryos.
    Authors: Kawaguchi J, Nichols J, Gierl MS, Faial T, Smith A
    Development, 2010;137(5):693-704.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Long-term culture of male germline stem cells from hamster testes.
    Authors: Kanatsu-Shinohara M, Muneto T, Lee J, Takenaka M, Chuma S, Nakatsuji N, Horiuchi T, Shinohara T
    Biol. Reprod., 2007;78(4):611-7.
    Species: Hamster
    Sample Type: Whole Cells
    Application: Bioassay
  17. Gdnf upregulates c-Fos transcription via the Ras/Erk1/2 pathway to promote mouse spermatogonial stem cell proliferation.
    Authors: He Z, Jiang J, Kokkinaki M, Golestaneh N, Hofmann MC, Dym M
    Stem Cells, 2007;26(1):266-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. Identification of neuregulin as a factor required for formation of aligned spermatogonia.
    Authors: Hamra FK, Chapman KM, Nguyen D, Garbers DL
    J. Biol. Chem., 2007;282(1):721-30.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  19. Purification of bioactive glycosylated recombinant glial cell line-derived neurotrophic factor.
    Authors: Garbayo E, Ansorena E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ
    Int J Pharm, 2007;344(1):9-15.
    Species: N/A
    Sample Type: N/A
    Application: WB Ctrl
  20. Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D.
    Authors: Murata T, Tsuboi M, Hikita K, Kaneda N
    J. Biol. Chem., 2006;281(32):22503-16.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Glial cell line-derived neurotrophic factor (GDNF) receptor alpha-1 (GFR alpha 1) is highly selective for GDNF versus artemin.
    Authors: Carmillo P, Dago L, Day ES, Worley DS, Rossomando A, Walus L, Orozco O, Buckley C, Miller S, Tse A, Cate RL, Rosenblad C, Sah DW, Gronborg M, Whitty A
    Biochemistry, 2005;44(7):2545-54.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  22. TRPA1 induced in sensory neurons contributes to cold hyperalgesia after inflammation and nerve injury.
    Authors: Obata K, Katsura H, Mizushima T, Yamanaka H, Kobayashi K, Dai Y, Fukuoka T, Tokunaga A, Tominaga M, Noguchi K
    J. Clin. Invest., 2005;115(9):2393-401.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 22 Citations
Recombinant Proteins
Description Application Cat# Citations Images  

Recombinant Rat GFR alpha-1 Fc Chimera Protein

BA, Bind 560-GR 9
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Recombinant Rat GFR alpha-1 Fc Chimera Protein, CF

BA, Bind 560-GR/CF 9
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 2 applications: Cell proliferation, Immunoassay Standard.
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  Excellent
 Cell proliferation Anonymous 05/10/2017
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ApplicationCell proliferation
  Excellent
 Immunoassay Standard Anonymous 05/06/2016
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ApplicationImmunoassay Standard

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