Recombinant Rat IL-7 Protein

Name: Recombinant Rat IL-7 Protein
Brand: R&D Systems
SKU: 7857-RL
Price: 362 USD
Availability: InStock

R&D Systems | Catalog # 7857-RL

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Product Documents

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Product Specifications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

R&D Systems E. coli-derived Recombinant Rat IL-7 Protein (7857-RL)
Quality control testing to verify active proteins with lot specific assays by in-house scientists
All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

NP_037242

Applications

Bioactivity

View all IL-7 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rat IL-7 protein
Asp26-Ile154, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Starts at Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

15 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894.
The ED₅₀ for this effect is 0.1-0.6 ng/mL.

Formulation, Preparation, and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 7857-RL

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Carrier Free: 7857-RL/CF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-7

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Rat IL‑7 cDNA encodes 154 amino acids (aa) that include a 25 aa signal peptide. Mature rat IL‑7 shares approximately 88% aa sequence identity with mouse IL‑7 and 58‑60% with human, equine, bovine, ovine, porcine, feline and canine IL‑7. Human and mouse IL‑7 exhibit cross‑species activity (2, 3). IL‑7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL‑7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL‑7 signals through a complex of the IL‑7 Receptor alpha subunit (IL‑7 R alpha, also known as CD127) with the common gamma chain ( gamma _c) (1). The gamma _c is also a subunit of the receptors for IL‑2, ‑4, ‑9, ‑15, and ‑21 (1). IL‑7 R alpha is expressed on double negative (CD4^-CD8^-) and CD4⁺ or CD8⁺ single positive naïve and memory T cells, but undergoes IL‑7‑mediated down‑regulation and shedding during antigen‑driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6‑11). IL‑7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl‑2 (9‑11). It is required for optimal T cell‑dendritic cell interaction (6). IL‑7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL‑7 activation of IL‑7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL‑7 functions in both mouse and human pro‑B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).