Recombinant Rat Jagged 1 Fc Chimera Protein, CF

  (12 citations)   
Product Details
Citations (12)
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  • Purity
    >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.1 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M. et al. (2005) J. Biol. Chem. 280:15842. The ED50 for this effect is 0.5-2.0 µg/mL.
  • Source
    Mouse myeloma cell line, NS0-derived
    Rat Jagged 1
    Ser32-Asp1068
    (Gly57-Arg59 del, Asp63Thr, Arg64Leu, and Val-Arg-Pro-Tyr ins before Lys69)
    Accession # Q63722.2
    IEGRMD Human IgG1
    (Pro100-Lys330)
    6-His tag
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Ser32
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    141.7 kDa (monomer)
  • SDS-PAGE
    180 kDa, reducing conditions
599-JG
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Jagged 1

Rat Jagged1 was the first ligand for Notch identified in mammals. Since both the ligands and receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Jagged1 is synthesized as a precursor protein that contains a 21 aa signal sequence, a 1048 aa extracellular region, a 25 aa transmembrane (TM) segment and a short, 226 aa cytoplasmic domain. The large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region (1). The extracellular region of rJagged1 binds to multiple Notch receptors on the cell surface as well as in solid phase binding studies. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors (2). Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis and development of the vasculature (3). For instance soluble, non-transmembrane forms of Jagged1 influence behavior in fibroblast cells leading to characteristics exhibited by endothelial cells during angiogenesis (4). Soluble Jagged1 is also capable of maintaining the survival and enhancing the expansion of human stem cells that are capable of reconstituting hematopoietic lineages in vivo (5). Furthermore, Jagged1 is implicated in human disease: Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, eye, skeletal, craniofacial tissues, and kidney, is caused by mutations in Jagged1 (6). Depending on cell types and how soluble forms of the ligand are presented, ligand binding can result in activation or inhibition of Notch signaling (7). Rat Jagged1 shows 98% and 99% aa identity to human and mouse Jagged1 extracellular domains respectively. Relative to the extracellular region of rat Jagged2, the aa identity is 58%.

  • References:
    1. Lindsell, C.E. et al. (1995) Cell 80:909.
    2. Shimizu, K. et al. (1999) J. Biol. Chem. 274:32961.
    3. Lewis, J. (1998) Stem Cell & Dev. Biol. 9:583.
    4. Small, D. et al. (2001) J. Biol. Chem. 276:32022.
    5. Karanu, F. et al. (2000) J. Exp. Med. 192:1365.
    6. Joutel, A. and E. Tounier-Lasserve (1998) Stem Cell & Dev. Biol. 9:619.
    7. Hicks, C. et al. (2002) J Neurosci. Res. 68:655.
  • Entrez Gene IDs:
    182 (Human); 29146 (Rat)
  • Alternate Names:
    AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Selective regulation of Notch ligands during angiogenesis is mediated by vimentin
    Authors: D Antfolk, M Sjöqvist, F Cheng, K Isoniemi, CL Duran, A Rivero-Mul, C Antila, R Niemi, S Landor, CVC Bouten, KJ Bayless, JE Eriksson, CM Sahlgren
    Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  2. CCN1 induces hepatic ductular reaction through integrin alphavbeta(5)-mediated activation of NF-kappaB.
    Authors: Kim K, Chen C, Alpini G, Lau L
    J Clin Invest, 2015;125(5):1886-900.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  3. MicroRNA-199b Modulates Vascular Cell Fate During iPS Cell Differentiation by Targeting the Notch Ligand Jagged1 and Enhancing VEGF Signaling.
    Authors: Chen T, Margariti A, Kelaini S, Cochrane A, Guha S, Hu Y, Stitt A, Zhang L, Xu Q
    Stem Cells, 2015;33(5):1405-18.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. beta-site amyloid precursor protein cleaving enzyme 1(BACE1) regulates Notch signaling by controlling the cleavage of Jagged 1 (Jag1) and Jagged 2 (Jag2) proteins.
    Authors: He W, Hu J, Xia Y, Yan R
    J Biol Chem, 2014;289(30):20630-7.
    Species: Rat
    Sample Type: Protein
    Application: Enzyme Substrate
  5. PKCzeta regulates Notch receptor routing and activity in a Notch signaling-dependent manner.
    Authors: Sjoqvist M, Antfolk D, Ferraris S, Rraklli V, Haga C, Antila C, Mutvei A, Imanishi S, Holmberg J, Jin S, Eriksson J, Lendahl U, Sahlgren C
    Cell Res, 2014;24(4):433-50.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  6. Notch1 signaling modulates neuronal progenitor activity in the subventricular zone in response to aging and focal ischemia.
    Authors: Sun F, Mao X, Xie L, Ding M, Shao B, Jin K
    Aging Cell, 2013;12(6):978-87.
    Species: Rat
    Sample Type: In Vivo
    Application: Bioassay
  7. A receptor-specific function for Notch2 in mediating vascular smooth muscle cell growth arrest through cyclin-dependent kinase inhibitor 1B.
    Authors: Boucher, Joshua M, Harrington, Anne, Rostama, Bahman, Lindner, Volkhard, Liaw, Lucy
    Circ Res, 2013;113(8):975-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Protective astrogenesis from the SVZ niche after injury is controlled by Notch modulator Thbs4.
    Authors: Benner, Eric J, Luciano, Dominic, Jo, Rebecca, Abdi, Khadar, Paez-Gonzalez, Patricia, Sheng, Huaxin, Warner, David S, Liu, Chunlei, Eroglu, Cagla, Kuo, Chay T
    Nature, 2013;497(7449):369-73.
  9. Attenuation of Notch signaling promotes the differentiation of neural progenitors into neurons in the hippocampal CA1 region after ischemic injury.
    Authors: Oya S, Yoshikawa G, Takai K, Tanaka JI, Higashiyama S, Saito N, Kirino T, Kawahara N
    Neuroscience, 2009;158(2):683-92.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  10. Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
    Authors: Elkabetz Y, Panagiotakos G, Al Shamy G, Socci ND, Tabar V, Studer L
    Genes Dev., 2008;22(2):152-65.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  11. Notch1 engagement by Delta-like-1 promotes differentiation of B lymphocytes to antibody-secreting cells.
    Authors: Santos MA, Sarmento LM, Rebelo M, Doce AA, Maillard I, Dumortier A, Neves H, Radtke F, Pear WS, Parreira L, Demengeot J
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(39):15454-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. Differentiation of ES cells into cerebellar neurons.
    Authors: Salero E, Hatten ME
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(8):2997-3002.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
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