Recombinant Rat LILRC1 Protein, CF

Catalog # Availability Size / Price Qty
9698-T4-050
Recombinant Rat LILRC1 Protein, CF Bioactivity
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Recombinant Rat LILRC1 Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Rat LILRC1 is immobilized at 2 μg/mL, 100 μL/well, Recombinant Human Angiopoietin‑like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.05-0.3 μg/mL.
Source
Mouse myeloma cell line, NS0-derived rat LILRC1 protein
Gln17-Asn248, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained. Gln17 inferred from enzymatic pyroglutamate treatment revealing Glu18
Predicted Molecular Mass
27 kDa
SDS-PAGE
34-41 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9698-T4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

When Recombinant Rat LILRC1 (Catalog # 9698-T4) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Angiopoietin-like Protein 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50of 0.05-0.3 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: LILRC1

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRC1 (leukocyte immunoglobulin-like receptor C1), also known as LILRA5, ILT11, LIR-9, and CD85f, consists of an extracellular domain (ECD), a transmembrane segment, and a cytoplasmic tail (2, 3). The ECD contains two Ig-like domains (4), and the transmembrane segment contains a positively charged arginine residue which may mediate its association with the signaling molecule, FcR common gamma chain (3, 5). Within the ECD, rat LILRC1 shares 58% and 85% amino acid sequence identity with human and mouse LILRA5, respectively. LILRC1 is expressed by monocytes, macrophages, and neutrophils (2, 3, 5). It is found as an approximately 40 kDa molecule on the cell surface, while a soluble 25 kDa form can be released into the synovial fluid of rheumatoid arthritis patients (2, 5). Cross-linking of LILRC1 on monocytes induces the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, TNF-alpha) as well as the anti-inflammatory IL-10 (2, 5). R&D Systems in-house testing indicates that LILRC1/LILRA5 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (6).

References
  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Borges, L. et al. (2003) Blood 101:1484.
  3. Hoelsbrekken, S.E. et al. (2005) Immunogenetics 57:479.
  4. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
  5. Mitchell, A. et al. (2008) Eur. J. Immunol. 38:3459.
  6. Zheng, J. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-like Receptor C1
Entrez Gene IDs
232801 (Mouse); 691533 (Rat)
Alternate Names
LILRA5; LILRC1

FAQs

No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reconstitution Buffers

Reviews for Recombinant Rat LILRC1 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Rat LILRC1 Protein, CF and earn rewards!

Have you used Recombinant Rat LILRC1 Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review