Recombinant Rat LILRC2 Protein, CF

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Recombinant Rat LILRC2 Protein Binding Activity
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Recombinant Rat LILRC2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Rat LILRC2 is immobilized at 2 μg/mL,100 μL/well, the concentration of Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) that produces 50% of the optimal binding response is 0.04-0.24 μg/mL
Mouse myeloma cell line, NS0-derived rat LILRC2 protein
Met1-Asn230, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
24 kDa
32-43 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Rat LILRC2 Protein Binding Activity View Larger

When Recombinant Rat LILRC2 (Catalog # 9808-T4) is immobilized at 2 µg/mL, 100 µL/well, Recombinant Human Angiopoietin-like 7/ANGPTL7 (Catalog # 914-AN) binds with an ED50 of 0.04-0.24 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: LILRC2

The leukocyte immunoglobulin-like receptors (LILR) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIRs) (1). LILRC2 (leukocyte immunoglobulin-like receptor C2) is a 35 kDa member of the LILRs that are expressed on hematopoietic cells. Mature rat LILRC2 is a type I transmembrane (TM) protein that is 241 amino acids (aa) in length. It contains a 207 aa extracellular domain (ECD), a 19 aa TM segment, and a short cytoplasmic tail (2). The ECD contains two Ig-like domains while the TM segment possesses an Arg residue (2, 3). This suggests that LILRC2 is an activating receptor that may interact with Fc epsilon RI gamma and/or CD3 zeta. Cells expressing LILRC2 include neutrophils, B cells, and CD4+ and CD8+ T cells (2). No human or mouse LILRC2 is known. Rat LILRC2 shares 47.9% identity with LILRC1. R&D Systems in-house testing indicates that LILRC2 binds to Angiopoietin-like 7, consistent with the demonstrated functional interactions between other members of these protein families (4).

  1. Thomas, R. et al. (2010) Clin. Rev. Allergy Immunol. 38:159.
  2. Hoelsbrekken, S.E. et al. (2005) Immunogenetics 57:479.
  3. Shiroishi, M. et al. (2006) J. Biol. Chem. 281:19536.
  4. Zhang, C.C. et al. (2012) Nature 485:656.
Long Name
Leukocyte Immunoglobulin-like Receptor C2
Entrez Gene IDs
690906 (Rat)
Alternate Names


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